Abstract: The present invention relates an in-vitro method for diagnosis and/or prognosis of a disease in a tissue sample obtained from a mammalian subject comprising (i) Determining the level of a first biomarker using matrix-assisted laser desorption/ionization (MALDI)-imaging in the sample; (ii) Performing a histochemical staining of the sample; (iii) Determining the ratio of the levels of a second biomarker and a reference marker using fluorescence in situ-hybridization (FISH); wherein the same sample is used in steps (i) to (iii).