Abstract: This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and floral cell-preferred or floral tissue-preferred promoters.

Abstract: The present disclosure provides compositions and methods for altering auxin accumulation in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to auxin efflux through editing of a Brachytic2 (br2) gene to introduce an antisense sequence or segment or a deletion or premature stop codon into the gene. Modified plant cells and plants having a dominant or semi-dominant allele reducing the expression or activity of a br2 gene product are further provided comprising improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types in the plant.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, ear fresh weight, and single kernel weight, and increased yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more molybdenum cofactor (Moco) biosynthesis polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: Provided are compositions and methods for reducing, disrupting, or altering ZmDA1 activity in corn plants. Methods and compositions are also provided for producing modifications in the ZmDA1 gene through mutagenesis and/or editing. Modified plant cells and plants having a modification in the ZmDA1 gene are further provided comprising improved characteristics, such as increased yield.

Abstract: Provided are compositions and methods for reducing, disrupting, or altering ZmGW2 activity in corn plants. Methods and compositions are also provided for producing modifications in the ZmGW2 gene through mutagenesis and/or editing. Modified plant cells and plants having a modification in the ZmGW2 gene are further provided comprising improved characteristics, such as increased yield.

Abstract: Provided are compositions and methods for reducing, disrupting, or altering ZmDA2 activity in corn plants. Methods and compositions are also provided for producing modifications in the ZmDA2 gene through mutagenesis and/or editing. Modified plant cells and plants having a modification in the ZmDA2 gene are further provided comprising improved characteristics, such as increased yield.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, single kernel weight, ear fresh weight, ear area, ear volume, ear length, number of kernels per ear, and yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more MADS-box polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: Provided are compositions and methods for altering TFL1 levels in soybean plants. Methods and compositions are also provided for altering the expression of genes related to the timing of terminal differentiation of stem tips through suppression, mutagenesis and/or editing of the TFL1 gene. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a TFL1 gene are further provided comprising reduced TFL1 levels and improved characteristics, such as reduced plant height and increased lodging resistance.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear fresh weight, ear area, ear volume, ear diameter, ear length, ear tip void, number of kernels per ear, single kernel weight, and yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more glutamate dehydrogenase (GDH) polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency, and enhanced drought tolerance or water use efficiency. Transgenic plants may include field crops as well as plant propagules, plant parts and progeny of such transgenic plants. Methods of making and using such transgenic plants are also provided. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed, and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, increased single kernel weight, increased ear fresh weight, increased number of florets, and mitigated flowering delay. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear area, ear diameter, ear length, green leaf number below ear, yield, single kernel weight, and/or ear fresh weight, and decrease in ear tip void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more proliferating cell nuclear antigen (PCNA) polypeptide and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits and yield relative to a control plant, such as increased ear area, increased ear volume, increased single kernel weight, increased ear fresh weight, increased ear diameter, increased ear length, increased yield, increased kernels per ear, decreased ear tip void, and decreased ear void. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more CONSTANS (CO) or CONSTANS-like (COL) polypeptides and a transgene encoding one or more MADS-box polypeptides, and can also have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: The present disclosure provides modified, transgenic, or genome edited/mutated corn plants that are semi-dwarf and have one or more improved ear traits relative to a control plant, such as increase in ear diameter, single kernel weight, ear fresh weight, ear area, ear volume, ear length, number of kernels per ear, and yield. The modified, transgenic, or genome edited/mutated corn plants comprise a transgene encoding one or more MADS-box polypeptides and have a reduced expression of one or more GA20 or GA3 oxidase genes. Also provided are methods for producing the modified, transgenic, or genome edited/mutated corn plants.

Abstract: This invention relates to compositions and methods for improving/enhancing yield traits by modifying cytokinin oxidase (CKX) levels in a plant. The invention further relates to plants produced using the methods and compositions of the invention.

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through suppression, mutagenesis and/or editing of specific subtypes of GA20 or GA3 oxidase genes. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a GA oxidase gene are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.

Abstract: The present disclosure provides compositions and methods for altering gibberellin (GA) content in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to gibberellin biosynthesis through editing of specific subtypes of GA20 oxidase genes to introduce an antisense sequence or segment into the gene. Modified plant cells and plants having a dominant allele reducing the expression or activity of one or more GA oxidase genes are further provided comprising reduced gibberellin levels and improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types.