Abstract: A sorting flow cytometer identifies an undesirable drop charge sequence that is preassigned to adjacent drops before the drops have separated from a fluid stream. An example of an undesirable drop charge sequence is a sequence of adjacent drops that are charged with sufficiently high opposing charges that, after the drops are formed, would result in merging of the adjacent drops. The sorting flow cytometer adjusts the assignment of drop charges to avoid the undesired drop charge sequence.

Abstract: Multi-peak events are evaluated by a flow cytometer to distinguish events associated with a single particle from events associated with multiple particles for proper characterization of the particles.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: A sorting flow cytometer identifies an undesirable drop charge sequence that is preassigned to adjacent drops before the drops have separated from a fluid stream. An example of an undesirable drop charge sequence is a sequence of adjacent drops that are charged with sufficiently high opposing charges that, after the drops are formed, would result in merging of the adjacent drops. The sorting flow cytometer adjusts the assignment of drop charges to avoid the undesired drop charge sequence.

Abstract: A method is provided for generating measurement parameters for a particle sample in a particle analyzer. The method includes, interrogating the particle sample with a triggering interrogator and one or more secondary interrogators respectively positioned along a length of an interrogation area, generating respective pulses based upon the interrogation of a first particle from the particle sample, determining a primary pulse detection window based upon a triggering pulse, determining a search interval to find a secondary pulse based upon factors including the primary pulse detection window and a laser delay, adjusting the search interval for laser delay variation dynamically based on the interrogation of the first particle, identifying the secondary pulse in the adjusted search interval, and processing the secondary pulse to determine a peak value of the secondary pulse. Corresponding apparatus are also provided.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: A method is provided for generating measurement parameters for a particle sample in a particle analyzer. The method includes, interrogating the particle sample with a triggering interrogator and one or more secondary interrogators respectively positioned along a length of an interrogation area, generating respective pulses based upon the interrogation of a first particle from the particle sample, determining a primary pulse detection window based upon a triggering pulse, determining a search interval to find a secondary pulse based upon factors including the primary pulse detection window and a laser delay, adjusting the search interval for laser delay variation dynamically based on the interrogation of the first particle, identifying the secondary pulse in the adjusted search interval, and processing the secondary pulse to determine a peak value of the secondary pulse. Corresponding apparatus are also provided.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: A flow cytometer capable of using electronics to analyze a large dynamic range while avoiding and/or correcting traditional limitations of acquisition electronics. The invention allows for measuring signal characteristics, which may include but are not limited to linear peak, integral, logarithmic peak, and pulse width. Other system parameters may also be generated by the invention to indicate timing or status information. Accurate and precise measurements may be performed by correcting for input errors if needed, and by transforming the input signal to produce a more easily measured form.

Abstract: Disclosed is an electronic processing system for a flow cytometer that uses a processing chip that processes data in a parallel architecture on a sample by sample basis and provides for high throughput of data. In addition, multi-gain linear amplifiers are used which are matched using feedback circuits to provide accurate data and high resolution data having high dynamic range.

Abstract: In one embodiment, selecting a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment. Steps of aligning a rectangular grid to screen region boundaries, wherein the screen includes a screen space divided into at least one screen region, locating a first and second endpoints of the straight line segment on the screen space, defining a rectangular bounding box in the screen space having vertices at the first and second endpoints, identifying each screen region that at least partially overlaps the bounding box, and selecting each identified screen region through which the straight line segment passes to activate for rendering the straight line segment on the screen are disclosed.

Abstract: In one embodiment, selecting auxiliary screen regions to activate in rendering a straight line segment on a screen of a computer monitor, wherein the straight line segment comprises points defined by an algebraic expression equal to zero, wherein the expression is expressed as a first constant multiplied by a x-axis value plus a second constant multiplied by a y-axis value plus a third constant, wherein the straight line segment has a width.

Abstract: In one embodiment, selecting a pixel in a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment by a rasterizer assigned to the screen region. Steps of aligning a rectangular grid to pixel boundaries, wherein the screen comprises a screen space divided into at least one screen region, wherein the screen region comprises multiple pixels, identifying each pixel in the screen region through which the straight line segment passes, and selecting each identified pixel to activate for rendering the straight line segment on the screen when the straight line segment passes through a diamond shaped area of the pixel whose boundary is defined by the lower, upper, left, and right pixel grid mid-points are disclosed.

Abstract: In one embodiment, selecting a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment. Steps of aligning a rectangular grid to screen region boundaries, wherein the screen includes a screen space divided into at least one screen region, locating a first and second endpoints of the straight line segment on the screen space, defining a rectangular bounding box in the screen space having vertices at the first and second endpoints, identifying each screen region that at least partially overlaps the bounding box, and selecting each identified screen region through which the straight line segment passes to activate for rendering the straight line segment on the screen are disclosed.

Abstract: In one embodiment, selecting a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment. Steps of aligning a rectangular grid to screen region boundaries, wherein the screen includes a screen space divided into at least one screen region, locating a first and second endpoints of the straight line segment on the screen space, defining a rectangular bounding box in the screen space having vertices at the first and second endpoints, identifying each screen region that at least partially overlaps the bounding box, and selecting each identified screen region through which the straight line segment passes to activate for rendering the straight line segment on the screen are disclosed.

Abstract: In one embodiment, selecting auxiliary screen regions to activate in rendering a straight line segment on a screen of a computer monitor, wherein the straight line segment comprises points defined by an algebraic expression equal to zero, wherein the expression is expressed as a first constant multiplied by a x-axis value plus a second constant multiplied by a y-axis value plus a third constant, wherein the straight line segment has a width.

Abstract: In one embodiment, selecting a pixel in a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment by a rasterizer assigned to the screen region. Steps of aligning a rectangular grid to pixel boundaries, wherein the screen comprises a screen space divided into at least one screen region, wherein the screen region comprises multiple pixels, identifying each pixel in the screen region through which the straight line segment passes, and selecting each identified pixel to activate for rendering the straight line segment on the screen when the straight line segment passes through a diamond shaped area of the pixel whose boundary is defined by the lower, upper, left, and right pixel grid mid-points are disclosed.

Abstract: In one embodiment, selecting a screen region on a screen of a monitor of a computer graphics display system to activate in rendering a straight line segment. Steps of aligning a rectangular grid to screen region boundaries, wherein the screen includes a screen space divided into at least one screen region, locating a first and second endpoints of the straight line segment on the screen space, defining a rectangular bounding box in the screen space having vertices at the first and second endpoints, identifying each screen region that at least partially overlaps the bounding box, and selecting each identified screen region through which the straight line segment passes to activate for rendering the straight line segment on the screen are disclosed.