Abstract: A method of predicting one or more resonance frequencies for efficient operation of an acoustofluidic cavity in an acoustofluidic device is provided. The method comprises the steps of i. providing a first liquid filling the cavity, ii. obtaining a first impedance spectrum, by measuring the electrical impedance of an ultrasound transducer connected to the acoustofluidic device as the transducer is driven at a range of frequencies, iii. providing a second liquid filling the cavity, wherein the second liquid differs from the first liquid in its acoustophysical properties, iv. obtaining a second impedance spectrum, by measuring the electrical impedance of the ultrasound transducer as the ultrasound transducer is driven at the range of frequencies, and v.

Abstract: A method of predicting one or more resonance frequencies for efficient operation of an acoustofluidic cavity in an acoustofluidic device is provided. The method comprises the steps of i. providing a first liquid filling the cavity, ii. obtaining a first impedance spectrum, by measuring the electrical impedance of an ultrasound transducer connected to the acoustofluidic device as the transducer is driven at a range of frequencies, iii. providing a second liquid filling the cavity, wherein the second liquid differs from the first liquid in its acoustophysical properties, iv. obtaining a second impedance spectrum, by measuring the electrical impedance of the ultrasound transducer as the ultrasound transducer is driven at the range of frequencies, and v.

Abstract: The invention provides a micro scaled method and system for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension. The suspension is forced under pressure into an inlet and further into a pre-alignment channel wherein the suspension is subjected to a two dimensional acoustic force directed perpendicular to the length direction of the pre-alignment channel. The suspension is then forced into a second inlet and further into at least one separation channel wherein the mixture of cells and/or particles are forced towards the walls of the channel and subjected to a one dimensional acoustic force directed perpendicular to the length direction of the separation channel. Cells and/or particles having the same size and/or mass density and/or compressibility are collected through at least two outlets.

Abstract: A microfluidic impedance flow cytometer (‘MIC’) device (2) comprises a substrate (4) in which is formed at least one flow channel (6) for leading through a particle (22) containing fluidic sample. The flow channel (6) is formed with a focusing zone (12) and a measurement zone (14) located downstream of the focusing zone (12) in the direction of through flow and provided with an electrode arrangement (18) for characterising particles (22) in the flowing fluidic sample by means of electrical impedance wherein an acoustophoretic particle focusing arrangement (20) is provided in acoustic coupling to the flow channel (6) in the focusing zone (12) to induce acoustic forces in fluid in the flow channel (6) so as to effect a lateral and/or vertical focusing of particles before flowing to the measurement zone (14).

Abstract: The invention relates to a method and system to separate a subgroup of cells from a mixture of cells. This is achieved by creating a complex consisting of the subgroup of cells that are to be separated and a ligand that could be constructed of molecules such as antibodies, monoclonal antibodies, aptamers or fragments thereof specific for said subgroup of cells and microbeads present in a suspension. The suspension is then exposed to an acoustic field in one or two dimensions, which forces said cells and cell-bead complexes to separate from each other based on differences in size, density and compressibility.

Abstract: The disclosure related to a method and system to separate a subgroup of objects from a mixture of objects present in a suspension wherein said suspension if exposed to acoustic forces acting in one or two dimensions, which forces said objects to separate from each other into groups which have similar properties in connection with size and/or mass density and/or compressibility.

Abstract: The invention relates to a biological microglia comprising at least two pores having a size adopted to allow cells, cell aggregates, tissue or other biological material to pass through said pores, and one or several slicing beams separating said pores from each other, wherein biological material is split/sliced/cleaved into at least two parts when passing said microgrid, a slicing device, an apparatus comprising said slicing device as well as the use of said microgrid, slicing device and apparatus.

Abstract: The invention relates to a device for manipulation of particles (30) in a sample liquid (32) said device comprising a source of ultrasound (16) capable of emitting ultrasound with a given wavelength, an inlet for a sample liquid (2), one or more outlets (4, 5, 6) and a compartment (14), being dimensioned to support a standing ultrasonic wave (40) of said wavelength, characterised in that the device further comprises an inlet for sheath liquid (1, 3) configured to direct a sheath liquid (34) to extend substantially in parallel to an anti-node plane (46) of the ultrasonic standing wave (40) proximate to a sheathed compartment wall. Specifically the device may be used in combination with a particle enumeration device for enumeration of somatic cells in milk.

Abstract: The present invention provides a method and apparatus for separating by size a mixture of different size particles using ultrasound. The apparatus contains a microchannel having an acoustic transducer thereon. As a mixture of cells having different sizes flows down the microchannel, the ultrasonic radiation traps cells of desired sizes focused at nodes of a standing pressure wave in the microchannel.

Abstract: The invention relates to a biological microglia comprising at least two pores having a size adopted to allow cells, cell aggregates, tissue or other biological material to pass through said pores, and one or several slicing beams separating said pores from each other, wherein biological material is split/sliced/cleaved into at least two parts when passing said microgrid, a slicing device, an apparatus comprising said slicing device as well as the use of said microgrid, slicing device and apparatus.

Abstract: The invention relates to a method for separation of elements from a fluid using affinity-bearing particles suspended in the fluid and using ultrasonic standing waves and micro-fluidics. The method includes the steps of: mixing said fluid mixture with particles (10) having affinity to at least one element (9) to be separated; allowing the element (9) to be separated to bind to said affinity-bearing particles (10); subjecting the fluid to an ultrasonic wave field resulting in forces on the affinity-bearing particles (10) but substantially no forces on elements not bound to affinity-bearing particles; and allowing said forces to move said affinity-bearing particles (10) to a portion of the fluid thus obtaining a locally higher concentration of affinity-bearing particles. The method may be performed in a process with continuous flow.

Abstract: The invention relates to devices and methods for chemical analysis, specifically to devices for extracting molecules, e.g. biomolecules such as peptides, and/or proteins, from a mixture of molecules in a solution and performing sample treatment on the extracted molecules before presenting them to an analysis instrument. The method for analysis of samples uses a combined sample treatment and sample carrier device. Said device comprises a plate with inlets at one side connected to respective compartments situated at respective array positions for receiving samples to be treated and analysed, each compartment being in communication with an outlet enabling fluid flow through the plate.

Abstract: The present invention provides a device and a method for separating particles from fluids using ultrasound, laminar flow, and stationary wave effects comprising a micro-technology channel system with an integrated branching point or branching fork, and a single ultrasound source. One of the characteristics of the invention is that the single ultrasound source, which generates the standing waves, excites the complete structure including the channel system. No special reflectors or the like are needed. Extremely thin dividers can separate the flow, thereby enhancing the effectiveness of the device. The device could be manufactured in silicon and the ultrasound energy could preferably be delivered by a piezoelectric element.

Abstract: The present invention relates to a microfluidic cell and method for sample handling, and more particularly a cell (1) with a one-dimensional or two-dimensional array of ultrasonic transmitters (2) or resonance cavities for trapping biologically activated microbeads and passing fluids carrying samples interacting with the microbeads for detection and analysis. The invention allows for individual loading of the positions in the cell and individual detection steps enabling multistep biological assays to be performed on submicrolitre volumes. The invention also relates to an apparatus and method for blood plasma analysis incorporating such a microfluidic cell.

Abstract: A processing module for extracting certain biomolecules from a solution, comprising an extraction unit having at least one elongated channel (101) each of said at least sine channel have un inlet (102) and an outlet (103) and being provided with adhering units (201), each said unit being provided with adhesive means, having affinity for said certain biomolecules, said extraction unit further comprises docking means (205) having an inlet array and an outlet array, that enables the extractor to be docked to and undocked from other devices having corresponding docking means, such that said solution or another fluid can be made to flow from said other devices, entering the inlet array, to flow through the at least one channels(101) and to leave the extraction device via the outlet array.

Abstract: A dispensing device for use in chemical analysis comprising at least two dispenser nozzles, a chamber having at least two inlets, a membrane entity constituting part of defining elements of said chamber, said membrane entity comprising at least one flexible membrane, and an actuation element, such that liquids brought to flow through said inlets into said chamber can be pressurised by actuating the membrane entity by providing a pulse to said actuation element, and thereby dispensing an amount of liquid through each of said at least two nozzles. Embodiments include devices comprising integrated free flow electrophoresis separation means.

Abstract: A method of selecting and identifying bio-molecules present in a bio-sample is disclosed. The method comprises the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the case of detection of said bio-molecules; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media. Detecting means are then used to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step. A device (1) for carrying out the method is also disclosed as is a protein chip library produced by the device or method.

Abstract: The invention relates to a method and a machine for biomolecule handling using an array dispenser. More particularly, the invention relates to a method and a machine in which one or two separation procedures are performed in parallel channels and the separated biomolecules are deposited on a two-dimensional target plate for analysis e.g. in a MALDI TOF MS device (Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry). A method of sample handling comprises the following steps: supplying (101) a number of samples to a multichannel separation device having an equal number of separation channels; separating (102) the samples in parallel in said number of separation channels; supplying said channels to an array dispenser after separation; dispensing (103) said channels in parallel on to a target plate maintaining the separation of the multichannel separation device.

Abstract: A device for conducting integrated sequential separation and enrichment of a mixture of sample proteins, comprising means for separation and means for enrichment, whereby free flow electrophoresis—isoelectric focussing (FFE-IEF), is arranged to take place within an etched system of basins and channels is preferably employed in the separation step, multichannel conduits are used to guide the separated protein fractions to the enrichment stage, and a solid phase micro-extraction procedure, in an optionally dockable format, is preferably employed in the enrichment step. The separated fractions are then preferably dispensed onto a MALDI-plate for subsequent matrix assisted laser desorption ionisation (MALDI) analysis.

Abstract: A device and method useable for fast processing and depositing of biomolecules. The method includes the following steps: receiving a first set of analytes to be analysed; passing in an array format, analytes through extraction means where said biomolecules adhere to solid phase means; washing said solid phase means with a washing liquid, leaving only said biomolecules; performing elution by passing portions of organic solvent through each said solid phase, forming eluates constituting a second set of analytes containing said biomolecules, as a result of said elution; dispensing, in an array format, said second set of analytes using a micro dispensing array; controlling said array to dispense precise amounts of each one of said second set of analytes on target areas of a target plate.