Abstract: Disclosed are methods for preparing mRNA and proteins from synthetic DNA.

Abstract: A method for characterizing a gastro-intestinal disease in a subject involves comparing ratios of expression levels of genes in a biological sample from a subject to references, wherein the gastro-intestinal disease is characterized based on a difference in the ratios of the expression values of genes in the biological sample from the subject as compared to the references.

Abstract: A method for characterizing multiple sclerosis in a subject involves comparing ratios of expression levels of genes in a biological sample from a subject to references, wherein the multiple sclerosis is characterized based on a difference in the ratios of the expression values of genes in the biological sample from the subject as compared to the references.

Abstract: A method for characterizing a gastro-intestinal disease in a subject involves comparing ratios of expression levels of genes in a biological sample from a subject to references, wherein the gastro-intestinal disease is characterized based on a difference in the ratios of the expression values of genes in the biological sample from the subject as compared to the references.

Abstract: A non-woven melt-blown filament medium includes a mass of essentially continuous melt-blown polymer filaments and an essentially continuous traversing melt-blown polymer filament extending through the mass. The mass has a depth dimension, a longitudinal dimension, and a latitudinal dimension. The mass includes a plurality of layers, each of the plurality of layers being generally oriented in the longitudinal and latitudinal dimensions. The traversing filament is generally oriented in the depth dimension and extends through at least one layer of the mass. In one embodiment, the mass is cylindrical in shape and the layers comprise concentric zones. The traversing filament is intentionally deposited and positioned to improve the physical properties of the mass.

Abstract: A non-woven depth filter cartridge includes a cylindrical mass of essentially continuous melt-blown polymer filaments and an essentially continuous traversing melt-blown polymer filament extending through the mass. The mass has a depth dimension, a longitudinal dimension, and a circumferential dimension. The mass includes a plurality of layers, each of the plurality of layers being generally oriented in the longitudinal and circumferential dimensions. The traversing filament is generally oriented in the depth dimension and extends through one or more layers of the mass. In one embodiment, the layers of the mass comprise concentric zones. The traversing filament is intentionally deposited and positioned to improve the physical properties of the filter cartridge.

Abstract: A method of continuously producing a melt-blown polymer filament mass includes producing a first set of melt-blown polymeric filaments, collecting the first set of filaments on a rotating collection device to form a tubular filament mass having a plurality of layers, and applying a second set of melt-blown polymeric filaments to the filament mass. The first set is produced generally in-line along an axis generally parallel to the collection device. The second set is deposited on the filament mass such that filaments of the second set extend through and engage a plurality of layers of the filaments of the first set. The method further includes urging the filament mass along the collection device to create a tubular filament mass of indefinite length with a first major surface and a second major surface. In one embodiment, the second set are applied in a sweeping motion.

Abstract: The presently claimed subject matter provides a method for detecting an autoimmune disorder in a subject by obtaining a biological sample from the subject; determining expression levels of at least two genes in the biological sample; and comparing the expression level of each gene with a standard, wherein the comparing detects the presence of an autoimmune disorder in the subject. Also provided are compositions and kits for carrying out the methods of the presently claimed subject matter.

Abstract: A non-woven melt-blown filament medium includes a mass of essentially continuous melt-blown polymer filaments and an essentially continuous traversing melt-blown polymer filament extending through the mass. The mass has a depth dimension, a longitudinal dimension, and a latitudinal dimension. The mass includes a plurality of layers, each of the plurality of layers being generally oriented in the longitudinal and latitudinal dimensions. The traversing filament is generally oriented in the depth dimension and extends through at least one layer of the mass. In one embodiment, the mass is cylindrical in shape and the layers comprise concentric zones. The traversing filament is intentionally deposited and positioned to improve the physical properties of the mass.

Abstract: A non-woven depth filter cartridge includes a cylindrical mass of essentially continuous melt-blown polymer filaments and an essentially continuous traversing melt-blown polymer filament extending through the mass. The mass has a depth dimension, a longitudinal dimension, and a circumferential dimension. The mass includes a plurality of layers, each of the plurality of layers being generally oriented in the longitudinal and circumferential dimensions. The traversing filament is generally oriented in the depth dimension and extends through one or more layers of the mass. In one embodiment, the layers of the mass comprise concentric zones. The traversing filament is intentionally deposited and positioned to improve the physical properties of the filter cartridge.

Abstract: A method of continuously producing a melt-blown polymer filament mass includes producing a first set of melt-blown polymeric filaments, collecting the first set of filaments on a rotating collection device to form a tubular filament mass having a plurality of layers, and applying a second set of melt-blown polymeric filaments to the filament mass. The first set is produced generally in-line along an axis generally parallel to the collection device. The second set is deposited on the filament mass such that filaments of the second set extend through and engage a plurality of layers of the filaments of the first set. The method further includes urging the filament mass along the collection device to create a tubular filament mass of indefinite length with a first major surface and a second major surface. In one embodiment, the second set are applied in a sweeping motion.

Abstract: A new non-woven depth filter cartridge is formed by insertion of a core into filter mass formed in a continuous process. A mass of very fine diameter polymer filaments is overlaid by mass of larger diameter polymer filaments. The filaments are continuously accumulated on a spinning mandrel and advanced along and off of the mandrel by a press roller. An inner cylindrical surface of the cumulative filament mass forming the depth filter element is calendered, which facilitates insertion of a core member.

Abstract: A new non-woven depth filter element is formed in a continuous process whereby a mass of very fine diameter polymer filaments overlaid by mass of larger diameter polymer filaments are continuously accumulated on a spinning mandrel and advanced along and off of the mandrel by a press roller. An inner cylindrical surface of the cumulative filament mass forming the depth filter element is calendered, which facilitates insertion of a core member.

Abstract: The present invention comprises new methods for treating rheumatoid arthritis. It has been found that the L-type calcium channel antagonists are effective in treating arthritis. Nimodipine, nisoldipine, and nifedipine, are examples of specific compounds useful in the present invention.

Abstract: The anthracene-spiro-pyrrolidines according to the invention are prepared by reacting either anthracene-spiro-furans or unsubstituted anthracene-spiropyrrolidines with appropriately substituted amines or by reacting unsubstituted anthracene derivatives with appropriately substituted methylenepyrrolidines. The substances can be used as active agents in medicaments, in particular for the production of immunomodulating medicaments.

Abstract: N,N-Disubstitued piperazines are prepared by reacting either the appropriately substituted furans or the N-unsubstituted pyrrolidines with aminoalkylpiperazines. The substances according to the invention can be employed as active agents in medicaments, in particular for the production of immunomodulators.

Abstract: Substituted pyrroloanthracenes are prepared by reacting either furanoanthracenes or N-unsubstituted pyrroloanthracenes with appropriately substituted alkylamines. The compounds can be employed as active ingredients in medicaments, in particular as immunomodulators.

Abstract: The piperazine-substituted pyrroloanthracenes are prepared by reacting either furanoanthracenes with piperazinalkylamines or reacting unsubstituted pyrroloanthracenes first with dihalogenoalkyl compounds and then with appropriately substituted piperazines or by reacting furan derivatives first with piperazinalkylamines and then condensing with anthracenes. The new compounds can be employed as active ingredients in medicaments, in particular in immunomodulators.

Abstract: A depth filter formed of a plurality of substantially continuous intertwined filaments including a central support zone formed of support filaments having a first diameter and a filter zone formed of filtration filaments having filaments of a second diameter in which the diameters are different or the filaments are constructed of different materials. The invention also relates to an apparatus and a method for making such a depth filter element.

Abstract: Human soluble immune response suppressor (SIRS) having a molecular weight of about 10,000 to 15,000, an isoelectric point of about 7 and a defined amino acid composition distinctly different from murine SIRS is produced from a culture of MOLT-4 cells.