Patents by Inventor Thomas Machleidt

Thomas Machleidt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240068009
    Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
    Type: Application
    Filed: August 29, 2023
    Publication date: February 29, 2024
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Publication number: 20240060059
    Abstract: Provided herein are circularly-permuted (cp) dehalogenase variants that are capable of covalently binding to a haloalkyl ligand. In particular cp dehalogenase variants and peptides and polypeptides comprising split versions thereof that structurally assemble to form an active dehalogenase complexes are provided.
    Type: Application
    Filed: May 4, 2023
    Publication date: February 22, 2024
    Inventors: Michael P. Killoran, Evan Glasgow, Lance P. Encell, Thomas Kirkland, Thomas Machleidt
  • Patent number: 11899021
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: January 4, 2023
    Date of Patent: February 13, 2024
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Publication number: 20230417778
    Abstract: Provided herein are compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
    Type: Application
    Filed: August 29, 2023
    Publication date: December 28, 2023
    Inventors: Carolyn W. Hitko, Thomas Kirkland, Thomas Machleidt, Rachel Friedman Ohana, Matt Robers, Keith Wood
  • Publication number: 20230382908
    Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.
    Type: Application
    Filed: April 5, 2023
    Publication date: November 30, 2023
    Inventors: Brock Binkowski, Mary P. Hall, Thomas Machleidt, Joel Walker, Wenhui Zhou
  • Publication number: 20230357266
    Abstract: Provided herein are compounds, compositions, systems, and methods for photoactivatable labeling, which can be actuated within biological systems. In particular, compounds disclosed herein include vinyl-extended-aryl azide moieties that undergo photoactivation to generate reactive intermediates, which can form covalent linkages with biomolecules. The photoactivation can be conducted by a variety of mechanisms including ultraviolet (UV) irradiation, visible light irradiation, or energy transfer (e.g., from a photocatalyst). The compounds also include functional moieties that provide useful functionalities, for example detection of biomolecules, such as fluorophores, capture elements (e.g., biotin), or reactive moieties (e.g., click handles) and bifunctional moieties comprising a bioactive compound and a detection/capture element.
    Type: Application
    Filed: May 4, 2023
    Publication date: November 9, 2023
    Inventors: Matthew A. Larsen, Rachel Friedman Ohana, Wenhui Zhou, Ce Shi, Jian Cao, Robin Hurst, Mark A. Klein, Hui Wang, Weiwei An, Karilyn Porter, Thomas Machleidt
  • Publication number: 20230341410
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Application
    Filed: January 4, 2023
    Publication date: October 26, 2023
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Publication number: 20230342826
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Application
    Filed: November 7, 2022
    Publication date: October 26, 2023
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • Patent number: 11781168
    Abstract: Described are processes for preparing substituted imidazo[1,2-a]pyrazine compounds of formula (I): by reacting a compound of formula (xi): with carbonyldiimidazole to form the compound of formula (I).
    Type: Grant
    Filed: April 26, 2021
    Date of Patent: October 10, 2023
    Assignee: PROMEGA CORPORATION
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Patent number: 11740249
    Abstract: Provided herein are compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: August 29, 2023
    Assignee: Promega Corporation
    Inventors: Carolyn W. Hitko, Thomas Kirkland, Thomas Machleidt, Rachel Friedman Ohana, Matt Robers, Keith Wood
  • Patent number: 11655247
    Abstract: Compounds that may inhibit Oplophorus-derived luciferases are disclosed as well as compositions and kits comprising the compounds and methods of using the compounds.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: May 23, 2023
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Mary P. Hall, Thomas Machleidt, Joel Walker, Wenhui Zhou
  • Publication number: 20230092932
    Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
    Type: Application
    Filed: November 21, 2022
    Publication date: March 23, 2023
    Inventors: Melanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
  • Patent number: 11567083
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: November 25, 2020
    Date of Patent: January 31, 2023
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Patent number: 11534504
    Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.
    Type: Grant
    Filed: October 3, 2019
    Date of Patent: December 27, 2022
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Thomas Smith, Thomas Kirkland, Thomas Machleidt, Keith Wood
  • Patent number: 11493504
    Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.
    Type: Grant
    Filed: April 17, 2020
    Date of Patent: November 8, 2022
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance Encell, Mary Hall, Keith Wood, Monika Wood, Marie Schwinn, Brock F. Binkowski, Hicham Zegzouti, Nidhi Nath, Subhanjan Mondal, Said Goueli, Poncho Meisenheimer, Thomas Kirkland, James Unch, Dileep K. Pulukkunat, Matthew Robers, Melanie Dart, Thomas Machleidt
  • Patent number: 11365402
    Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: June 21, 2022
    Assignee: Promega Corporation
    Inventors: Andrew S. Dixon, Lance P. Encell, Thomas Machleidt, Marie Schwinn, Keith Wood, Monika Wood, Kris Zimmerman
  • Publication number: 20210403886
    Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.
    Type: Application
    Filed: May 15, 2018
    Publication date: December 30, 2021
    Inventors: Andrew S. Dixon, Lance P. Encell, Thomas Machleidt, Marie Schwinn, Keith Wood, Monika Wood, Kris Zimmerman
  • Publication number: 20210254128
    Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
    Type: Application
    Filed: April 26, 2021
    Publication date: August 19, 2021
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Patent number: 11015216
    Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods of making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
    Type: Grant
    Filed: May 4, 2020
    Date of Patent: May 25, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Publication number: 20210080470
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Application
    Filed: November 25, 2020
    Publication date: March 18, 2021
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi