Abstract: The present disclosure provides methods and compositions for the improved expression of target proteins via the co-expression of an enhancer protein in a subject. Provided herein are methods for expressing a target protein in a subject comprising administering a vector system of one or more polynucleotides encoding a target protein and an enhancer protein, wherein the polynucleotides are operatively linked, and wherein the enhancer protein is an inhibitor of nucleocytoplasmic transport (NCT) and/or the enhancer protein is selected from the group consisting of a picornavirus leader (L) protein, a picornavirus 2A protease, a rhinovirus 3C protease, a herpes simplex virus (HSV) ICP27 protein, and a rhabdovirus matrix (M) protein.

Abstract: The invention relates to newly characterized light-inducible inward proton pumps and their use in medicine, their utility as optogenetic tools, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses.

Abstract: The present invention relates to a mutant ion channel capable of being activated by light (‘light-activated’ ion channel) and having improved properties, nucleic acids and expression vectors encoding the mutant ion channel, cells comprising such nucleic acid or expression vector, devices containing the mutant ion channel, nucleic acid or expression vector as well as respective uses and methods.

Abstract: The invention relates to mutant light-inducible ion channels having improved properties as compared to the parent channel, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising the nucleic acid construct or expression vector, and their respective uses, as well as non-human animals comprising the mutant light-inducible ion channel, the nucleic acid construct or the expression vector as disclosed herein.

Abstract: A stacked plate heat exchanger may include a plurality of stacked plates stacked on top of one another, between which a plurality of hollow spaces for two media may be alternately defined. The plurality of stacked plates may include at least two first stacked plates and at least one second stacked plate. The plurality of stacked plates may respectively include at least one first passage opening surrounded by a dome and at least one second passage opening. The at least one second passage opening of the at least one second stacked plate may be surrounded by an annular bead. The at least one second stacked plate may be arranged between the at least two first stacked plates such that the dome of a lower first stacked plate, the dome of an upper first stacked plate, and the annular bead are connected to one another and define an immersion tube.

Abstract: The invention relates to newly characterized light-inducible inward proton pumps and their use in medicine, their utility as optogenetic tools, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses.

Abstract: A stacked plate heat exchanger may include a plurality of stacked plates stacked on top of one another, between which a plurality of hollow spaces for two media may be alternately defined. The plurality of stacked plates may include at least two first stacked plates and at least one second stacked plate. The plurality of stacked plates may respectively include at least one first passage opening surrounded by a dome and at least one second passage opening. The at least one second passage opening of the at least one second stacked plate may be surrounded by an annular bead. The at least one second stacked plate may be arranged between the at least two first stacked plates such that the dome of a lower first stacked plate, the dome of an upper first stacked plate, and the annular bead are connected to one another and define an immersion tube.

Abstract: The invention relates to mutant light-inducible ion channels having improved properties as compared to the parent channel, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses, as well as non-human animals comprising the mutant light-inducible ion channel, the nucleic acid construct or the expression vector as disclosed herein.

Abstract: The invention relates to mutant light-inducible ion channel having improved properties as compared to the parent channel, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses, as well as non-human animals comprising the mutant light-inducible ion channel, the nucleic acid construct or the expression vector as disclosed herein.

Abstract: The invention relates to a condenser of stacked-plate design, having a first flow duct for a refrigerant and having a second flow duct for a coolant.

Abstract: The invention relates to a condenser of stacked plate design, having a first flow channel for a refrigerant and a second flow channel for a coolant, wherein a plurality of plate elements is provided, which form channels adjacent to each other between the plate elements when the plate elements are stacked on top of each other.

Abstract: The invention relates to a condenser of stacked plate design, having a first flow channel for a refrigerant and a second flow channel for a coolant, wherein a plurality of plate elements is provided, which form channels adjacent to each other between the plate elements when the plate elements are stacked on top of each other.

Abstract: The invention relates to a condenser of stacked-plate design, having a first flow duct for a refrigerant and having a second flow duct for a coolant.

Abstract: A device (12) supplies energy to a rapid cycling and/or rapidly cycled integrated circuit (13, 52) which includes a circuit load (17) and an internal capacity (15) connected parallel to the circuit load (17). The integrated circuit (13, 52) has a high cycle frequency (f1) especially at least in the MHz range. A supply unit (14) especially designed as a current source is directly connected to the internal capacity (15). The supply unit (14) has an internal resistance, the impedance level of which is so high at the cycle frequency (f1) that a current (ID2) supplying the circuit load (17) originates to a greater degree from the internal capacity (15) than from the supply unit (14). At least one auxiliary load, current sink or load controller is provided as an integral component of the integrated circuit and is connected to the circuit load to smooth load fluctuations.

Abstract: The device (12) is used to supply energy to a rapid cycling and/or rapidly cycled integrated circuit (13) which comprises a circuit load (17) and an internal capacity (15) which is switched in parallel to the circuit load (17). The integrated circuit (13) has a high cycle frequency (f1) which in particular lies at least in the MHz range. A supply unit (14) which is in particular designed as a current source is directly connected to the internal capacity (15). The supply unit (14) has an internal resistance, the impedance level of which is so high with the cycle frequency (f1) that a current (ID2) which supplies the circuit load (17) originates to a greater degree from the internal capacity (15) than from the supply unit (14). At least one auxiliary load (54) which is arranged parallel to the circuit load (17) and which can be connected and disconnected, or a current sink, is provided, wherein the auxiliary load (54) or the current sink is in particular an integral component of the circuit (52) respectively.

Abstract: A sensor system includes a sensor which is connected to an analyzer, which powers the sensor and analyzes a measuring signal generated by the sensor using an analyzer circuit. To simplify the interconnection of sensor and analyzer and make different ground-side connection variants possible, the analyzer circuit is situated on the power supply side of the sensor.

Abstract: The resistances of resistors arranged in a matrix form in a pressure-sensitive resistor mat are measured with high accuracy and using simple circuitry by the fact that the output of an operational amplifier is connected to each row conductor and each column conductor, which are connected to the resistor. A voltage is selectively applied to the individual resistors by appropriately activating the operational amplifiers. Each operational amplifier associated with the row or column conductors is equipped with a current balancing circuit which detects the output current of the operational amplifier connected to the selected resistor which flows through this resistor. A processor determines the individual resistance values from the currents flowing through the individual resistors and the voltage drops across those resistors.

Abstract: In a device for triggering an electric power component, both open-loop voltage control or closed-loop voltage control, and open-loop current control or closed-loop current control of the switching output are provided for a switching operation of the power component.

Abstract: A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-.beta.-hydroxybutyrate, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with D-.beta.-hydroxybutyrate to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising a) an indicator dye that is responsive to thiols, such as a substituted isobenzothiazolone, Ellman's reagent or a derivative of Ellman's reagent; b) D-.beta.-hydroxybutyrate dehydrogenase; c) lipoamide dehydrogenase; d) D,L-lipoamide; and e) nicotinamide adenine dinucleotide, is incorporated into the carrier matrix to provide an accurate and sensitive assay of a test sample for D-.beta.-hydroxybutyrate (DHBA) in particular, and for ketone bodies in general.

Abstract: A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-.beta.-hydroxybutyrate, in a test sample are disclosed. The test device includes a test pad comprising a suitable carrier matrix incorporating an indicator reagent composition capable of interacting with D-.beta.-hydroxybutyrate to produce a detectable or measurable response. In addition, a new and improved indicator reagent composition, comprising a) an indicator dye that is responsive to thiols, such as a substituted isobenzothiazolone, Ellman's reagent or a derivative of Ellman's reagent: b) D-.beta.-hydroxybutyrate dehydrogenase; c) lipoamide dehydrogenase; d) D,L-lipoamide; and e) nicotinamide adenine dinucleotide, is incorporated into the carrier matrix to provide an accurate and sensitive assay of a test sample for D-.beta.-hydroxybutyrate (DHBA) in particular, and for ketone bodies in general.