Patents by Inventor Thomas Mansell

Thomas Mansell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Glycosylated protein expression in prokaryotes
    Patent number: 9512434
    Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: December 6, 2016
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Matthew Delisa, Cassandra Guarino, Thomas Mansell, Adam Fisher
  • GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES
    Publication number: 20150232861
    Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.
    Type: Application
    Filed: February 24, 2015
    Publication date: August 20, 2015
    Inventors: Matthew DELISA, Cassandra GUARINO, Thomas MANSELL, Adam FISHER
  • Glycosylated protein expression in prokaryotes
    Patent number: 8999668
    Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.
    Type: Grant
    Filed: January 5, 2009
    Date of Patent: April 7, 2015
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Matthew DeLisa, Cassandra Guarino, Thomas Mansell, Adam Fisher
  • SYSTEM USEFUL FOR REPORTING PROTEIN-PROTEIN INTERACTIONS IN THE BACTERIAL PERIPLASM
    Publication number: 20110287963
    Abstract: One aspect of the present invention relates to a reporter system for detection of protein-protein interactions in the periplasm of a prokaryotic host cell. The reporter system includes a first expression system which has a nucleic acid molecule encoding a first fragment of a reporter protein molecule, a nucleic acid molecule encoding a first signal sequence, and a nucleic acid molecule encoding a first member of a putative binding pair, where the nucleic acid molecule encoding the first fragment, the nucleic acid molecule encoding the first signal sequence, and the nucleic acid molecule encoding the first member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a first fusion protein.
    Type: Application
    Filed: November 16, 2009
    Publication date: November 24, 2011
    Applicant: CORNELL UNIVERSITY
    Inventors: Matthew P. Delisa, Thomas Mansell
  • GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES
    Publication number: 20110039729
    Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.
    Type: Application
    Filed: January 5, 2009
    Publication date: February 17, 2011
    Applicant: CORNELL RESEARCH FOUNDATION, INC.
    Inventors: Matthew Delisa, Cassandra Guarino, Thomas Mansell, Adam Fisher