Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: One aspect of the present invention relates to a reporter system for detection of protein-protein interactions in the periplasm of a prokaryotic host cell. The reporter system includes a first expression system which has a nucleic acid molecule encoding a first fragment of a reporter protein molecule, a nucleic acid molecule encoding a first signal sequence, and a nucleic acid molecule encoding a first member of a putative binding pair, where the nucleic acid molecule encoding the first fragment, the nucleic acid molecule encoding the first signal sequence, and the nucleic acid molecule encoding the first member of the putative binding pair are operatively coupled to permit their expression in a prokaryotic host cell as a first fusion protein.

Abstract: The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format.

Abstract: A method and system of gating for a medical imaging system includes utilizing a non-electrical sensor to acquire information for gating. A sensor assembly usable in the method and system of gating may include a non-electrical sensor coupled to one side of a patient-sensor interface, the other side adapted for securing to a patient.

Abstract: An MR system isolation impedance mismatch apparatus (12) includes an impedance mismatch layer (24). The impedance mismatch layer (24) performs as a mechanical notch filter and isolates an MR system component (14) from a surrounding structure (18). A method of tuning the impedance mismatch apparatus (12) includes determining a default notch filter frequency range. The impedance mismatch apparatus (12) is formed and performs as a notch filter having the default notch filter frequency range. The impedance mismatch apparatus (12) is installed and tested between the MR system component (14) and the surrounding structure (18). Vibrations are detected in the MR system component (14) and in the surrounding structure (18). The impedance mismatch apparatus is adjusted in response to the detected vibrations.

Abstract: A method and system of gating for a medical imaging system includes utilizing a non-electrical sensor to acquire information for gating. A sensor assembly usable in the method and system of gating may include a non-electrical sensor coupled to one side of a patient-sensor interface, the other side adapted for securing to a patient.

Abstract: A low noise imaging apparatus for producing Magnetic Resonance (MR) images of a subject and for substantially minimizing acoustic noise generated during imaging is provided. The imaging apparatus comprises a magnet assembly, a gradient coil assembly, and a rf coil assembly, wherein at least one of the magnet assembly, the gradient coil assembly and the rf coil assembly are configured to reduce the generation and transmission of acoustic noise.

Abstract: Vibration and noise isolation for a magnetic resonance imager (MRI) superconducting magnet cryocooler includes a bellows between the cryocooler and its vacuum sleeve in the magnet, a mass suspended along the axis of, but at a distance from, the cryocooler, elastomeric blocks parallel to the axis, and braided copper wires connecting the vacuum sleeve and the heat shields of the MRI. Motion limiters and bellows flexures are provided for dynamic loads which might be encountered during transport of the magnet.

Abstract: A magnetic-resonance-imaging (MRI) scanner subassembly. In one subassembly, a preferably annularly-cylindrical-shaped enclosure contains a first vacuum, and an MRI gradient coil assembly is located within the enclosure in the first vacuum. Preferably, an annularly-cylindrical housing is included which is coaxially aligned with the enclosure and contains a second vacuum which is higher than the first vacuum, and an MRI superconductive main coil is located within the housing in the second vacuum. In another subassembly, an MRI gradient coil assembly has a threshold excitation frequency, and an isolation mount assemblage supports the MRI gradient coil assembly.