Patents by Inventor Thomas P. Graycar
Thomas P. Graycar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6653093Abstract: Enzyme granulates contain besides the enzyme water-soluble and water-insoluble fillers, binders and if required other additives for granulates. In order to stabilize the enzyme, an alkaline or earth alkaline metal formiate is added, as well as reducing sugar, if required. These enzyme granulates may contain all usual enzymes for washing and cleaning purposes, in particular alkaline proteases. A process for preparing these enzyme granulates, as well as their use in powdery washing product compositions are also disclosed.Type: GrantFiled: June 13, 2000Date of Patent: November 25, 2003Assignee: Genencor International, Inc.Inventors: Peter F. Plank, Thomas P Graycar, Matthew J. Flynn
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Patent number: 6586221Abstract: Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc.Type: GrantFiled: October 13, 1994Date of Patent: July 1, 2003Assignee: Genencor International, Inc.Inventors: Thomas P. Graycar, Richard R. Bott, Lori J. Wilson
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Publication number: 20030119172Abstract: Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55° C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25° C.).Type: ApplicationFiled: August 8, 2002Publication date: June 26, 2003Applicant: The Regents of the University of CaliforniaInventors: You-Lo Hsieh, Mary Michelle Hartzell, Matthew G. Boston, Kathleen A. Clarkson, Katherine D. Collier, Thomas P. Graycar, Edmund A. Larenas
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Publication number: 20030077807Abstract: Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc.Type: ApplicationFiled: October 13, 1994Publication date: April 24, 2003Inventors: THOMAS P. GRAYCAR, RICHARD R. BOTT, LORI J. WILSON
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Publication number: 20020115193Abstract: Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55° C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25° C.).Type: ApplicationFiled: December 27, 1999Publication date: August 22, 2002Inventors: YOU-LO HSIEH, MARY MICHELLE HARTZELL, MATTHEW G. BOSTON, KATHLEEN A. CLARKSON, KATHERINE D. COLLIER, THOMAS P. GRAYCAR, EDMUND A. LARENAS
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Patent number: 6436696Abstract: Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55° C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25° C.).Type: GrantFiled: December 27, 1999Date of Patent: August 20, 2002Assignees: The Regents of the University of California, Genencor International, Inc.Inventors: You-lo Hsieh, Mary Michelle Hartzell, Matthew G. Boston, Kathleen A. Clarkson, Katherine D. Collier, Thomas P. Graycar, Edmund A. Larenas
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Patent number: 6431370Abstract: Detergent formulations are prepared by directly agglomerating a fermentation broth extract, containing a detergent-type enzyme and a nonionic detergent-type surfactant, with a suitable detergent base mixture, without need for prior isolation of the enzyme.Type: GrantFiled: June 8, 2000Date of Patent: August 13, 2002Assignee: Genencor International, Inc.Inventors: Edit L. Braunstein, Nathaniel T. Becker, Thomas P. Graycar
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Patent number: 6329332Abstract: The present invention provides novel phenol oxidizing enzymes naturally-produced by strains of the species Pleurotus ostreatus which possess a pH optima in the alkaline range and which are useful in modifying the color associated with dyes and colored compounds at alkaline pH.Type: GrantFiled: December 23, 1998Date of Patent: December 11, 2001Assignee: Genencor International, Inc.Inventors: William S. Borneman, Elizabeth A. Bodie, Thomas P. Graycar, Deborah S. Winetzky
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Patent number: 6277617Abstract: Modified enzymes are provided in which at least one amino acid, such as asparagine, leucine, methionine or serine, of an enzyme is replaced with a cysteine and the thiol hydrogen is replaced with a substituent group providing a thiol side chain selected from the group consisting of: a) —SR1R2, wherein R1 is an alkyl and R2 is a charged or polar moiety; b) —SR3, wherein R3 is a substituted or unsubstituted phenyl; c) —SR4, wherein R4 is substituted or unsubstituted cyclohexyl; d) —SR5, wherein R5 is C10-C15 alkyl; and e) —SR6 wherein R6 is a C1-6 alkyl. Also, methods of producing the modified enzymes are provided, as well as detergent and feed additives and a composition for the treatment of a textile. A method for using the modified enzymes in organic synthesis is additionally provided. Further, modified enzymes having improved activity, altered pH profile and/or wash performance are provided.Type: GrantFiled: November 25, 1997Date of Patent: August 21, 2001Assignees: Genencor International, Inc.Inventors: Richard R. Bott, Thomas P. Graycar, J. Bryan Jones, Colin Mitchinson
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Patent number: 6105786Abstract: A process is disclosed for preparing a detergent powder containing enzymes where the enzymes are extracted from a fermentation broth with a salt and surfactant mixture and directly agglomerated with detergent paste and dried to form the detergent powder. The process results in a two phase system where the enzyme is extracted into the surfactant rich phase and the second phase is salt rich. The process is especially useful for whole or clarified fermentation broths.Type: GrantFiled: July 2, 1997Date of Patent: August 22, 2000Assignee: Genencor International, Inc.Inventors: Edit L. Braunstein, Nathaniel T. Becker, Grant C. Ganshaw, Thomas P. Graycar
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Patent number: 6066494Abstract: Textile fibers are treated with enzymes in the absence of surfactants, with the effect of increasing the wettability and absorbency of the fibers. The enzymes are pectinases, cellulases, proteases, lipases or combinations thereof. The wetting properties of cotton fibers are found to be most substantially improved by treatment with a mixture of cellulase and pectinase. The effects of five hydrolyzing enzymes on improving the hydrophilicity of several polyester fabrics have been studied. Four out of the five lipases studied improve the water wetting and absorbent properties of the regular polyester fabrics more than alkaline hydrolysis under optimal conditions (3N NaOH at 55.degree. C. for 2 hours). Compared to aqueous hydrolysis, the enzyme reactions have shown to be effective under more moderate conditions, including a relatively low concentration (0.01 g/L), a shorter reaction time (10 minutes), at an ambient temperature (25.degree. C.).Type: GrantFiled: March 16, 1998Date of Patent: May 23, 2000Assignee: The Regents of the University of CaliforniaInventors: You-lo Hsieh, Mary Michelle Hartzell, Matthew G. Boston, Kathleen A. Clarkson, Katherine D. Collier, Thomas P. Graycar, Edmund A. Larenas
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Patent number: 5316941Abstract: There are described certain DNA sequences which encode subtilisins wherein the amino acid sequence of such substilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine and serine, has been substituted for the amino acid residues naturally occuring at such position.Type: GrantFiled: April 29, 1992Date of Patent: May 31, 1994Assignee: Genencor, Inc.Inventors: David A. Estell, Robert M. Caldwell, Richard R. Bott, Thomas P. Graycar
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Patent number: 5204015Abstract: Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: October 9, 1992Date of Patent: April 20, 1993Assignee: Genencor International, Inc.Inventors: Robert M. Caldwell, David A. Estell, Thomas P. Graycar
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Patent number: 5185258Abstract: Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: October 19, 1990Date of Patent: February 9, 1993Assignee: Genencor International, Inc.Inventors: Robert M. Caldwell, David A. Estell, Thomas P. Graycar
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Patent number: 5155033Abstract: There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.Type: GrantFiled: January 6, 1989Date of Patent: October 13, 1992Assignee: Genencor, Inc.Inventors: David A. Estell, Robert M. Caldwell, Richard R. Bott, Thomas P. Graycar