Abstract: Disclosed herein are engineered AAV VP capsid polypeptides with the ability to assemble into virus particles and having improved tissue tropism to, for example, CNS tissues. The capsids are engineered using the high throughput discovery system described herein. In certain embodiments, provided herein are recombinant adeno-associated virus (AAV) VP capsid polypeptides having at least one mutation in a residue corresponding to residue 581 to residue 589 in SEQ ID NO: 1.

Abstract: Disclosed herein are engineered AAV VP capsid polypeptides with the ability to assemble into virus particles and having improved tissue tropism to, for example, CNS tissues or muscle tissues. The capsids are engineered using the high throughput discovery system described herein. In certain embodiments, provided herein are recombinant adeno-associated virus (AAV) VP capsid polypeptides having at least one mutation in a 581-589 region of the VP capsid polypeptide, corresponding to residues 581 to residue 589 of a VP1 polypeptide of SEQ ID NO: 1.

Abstract: Disclosed herein are engineered AAV VP capsid polypeptides with the ability to assemble into virus particles and having improved tissue tropism to eye tissues, for example, retinal tissues, retinal pigment epithelial cells, or photoreceptor cells. The capsids are engineered using the high throughput discovery system described herein. In certain embodiments, provided herein are recombinant adeno-associated virus (AAV) VP capsid polypeptides having at least one mutation in a 581 to 589 region of the VP capsid polypeptide, corresponding to residues 581 to residue 589 of a VP1 polypeptide of SEQ ID NO: 1.

Abstract: Disclosed herein are engineered guide RNAs, engineered polynucleotides, precursor engineered polynucleotide, vectors omprising engineered polynucleotide, nucleic acids of engineered polynucleotide, pharmaceutical compositions thereof, methods of making the engineered polynucleotides and methods of treating or preventing a disease or condition by administering above described thereof.

Abstract: Disclosed herein are engineered AAV VP capsid polypeptides with the ability to assemble into virus particles and having improved tissue tropism to, for example, CNS tissues. The capsids are engineered using the high throughput discovery system described herein. In certain embodiments, provided herein are recombinant adeno-associated virus (AAV) VP capsid polypeptides having at least one mutation in a residue corresponding to residue 581 to residue 589 in SEQ ID NO: 1.

Abstract: The present invention is directed to a protective device including a fault protection circuit configured to provide a fault detection stimulus in response to detecting at least one type of predetermined fault condition. A circuit interrupter is disposed on a first side of a PCB and an auxiliary switch is at least partially disposed on a second side of the at least one PCB. The auxiliary switch includes a switch throw element movably extending through an opening in the PCB between the first state and the second state, the auxiliary switch being configured to decouple at least a portion of the fault protection circuit assembly from the plurality of line terminals in the second state.

Abstract: The present invention is directed to a protective device that includes a cover assembly having a plurality of receptacle openings, a reset button and a test button. The reset button and the test button are disposed along a first diagonal and in opposite corners of a substantially square area defined by the reset button and test button. At least one receptacle contact structure includes a portion extending across the first diagonal and at least one receptacle contact substantially aligned with one of the receptacle openings. An electromechanical apparatus is disposed in the back body within a region substantially aligned with the substantially square area. The electromechanical apparatus includes a toroidal sensor assembly coupled to an actuator coil arranged along a center axis in substantial alignment with the first diagonal, the center axis forming an acute angle relative to a side wall of the back body.

Abstract: The present invention is directed to a protective device that includes a cover assembly having a plurality of receptacle openings, a reset button and a test button. The reset button and the test button are disposed along a first diagonal and in opposite corners of a substantially square area defined by the reset button and test button. At least one receptacle contact structure includes a portion extending across the first diagonal and at least one receptacle contact substantially aligned with one of the receptacle openings. An electromechanical apparatus is disposed in the back body within a region substantially aligned with the substantially square area. The electromechanical apparatus includes a toroidal sensor assembly coupled to an actuator coil arranged along a center axis in substantial alignment with the first diagonal, the center axis forming an acute angle relative to a side wall of the back body.

Abstract: A system for enhancing sound quality comprising a filter that square roots the instantaneous amplitude of frequencies in an input signal for generating artificial harmonics corresponding to said frequencies. The system can comprise an automatic level control that momentarily boosts the amplitude of a higher frequency portion of the input signal to emphasize attack transients occurring within the input signal.

Abstract: A duplex container is constructed to allow the recording and measuring of time or other parameters by means of indices and associated surrounding moveable, adjustable elastomeric marker bands that are on and hold to the outer surface of the outer container. The outer container can hold an inner container that may be of similar shape.

Abstract: A snag releasing fishing hook and lure is made by constructing the hook and lure with an alternate/secondary line attachment point that is located behind the point/barb of the hook, such that if the main/primary line breaks from a debris/snag catch, the hook will twist/rotate and release from the catch by the function of the alternate/secondary line. If no secondary line is attached and the primary/main line is sufficiently strong the hook can be made fail and release from the snag without the loss of the lure.

Abstract: The present invention is directed to a protective device that includes a plurality of line terminals configured to be connected to an electrical distribution system, and a plurality of load terminals configured to be connected to a load. The device includes a fault detection circuit coupled to the plurality of line terminals and the plurality of load terminals. The fault detection circuit is configured to detect at least one fault in the electrical distribution system. A power interruption circuit couples the plurality of line terminals to the plurality of load terminals to thereby provide power to the load under normal operating conditions. The power interruption circuit also is coupled to the fault detection circuit, and configured to decouple the plurality of line terminals from the plurality of load terminals in response to the fault detection circuit detecting the at least one fault. A test circuit is coupled to the fault detection circuit and the power interruption circuit.

Abstract: A mineplough (1) which is designed to be resilient to exploding mines comprises one or more blades set at an angle to the general direction of movement of the plough so as to deflect lifted earth to one or both sides of the plough and having tines (5b-5j) arranged so as to lift earth ahead of the blade in the direction of travel thereof, depth control means for controlling the depth of cut of the tines and at least two linkages to connect the blade to a suitable carrier vehicle for the mineplough. The blade is comprised of a number of intersecting strong metal plates (2a-2j; 3a-3d) whose planes lie substantially parallel to the direction of travel of the mineplough and which define relatively narrow open channels therebetween so as to allow venting of any explosion caused by a mine exploding in contact with the blade.

Abstract: The present invention is directed to a protective device that includes a plurality of line terminals configured to be connected to an electrical distribution system, and a plurality of load terminals configured to be connected to a load. The device includes a fault detection circuit coupled to the plurality of line terminals and the plurality of load terminals. The fault detection circuit is configured to detect at least one fault in the electrical distribution system. A power interruption circuit couples the plurality of line terminals to the plurality of load terminals to thereby provide power to the load under normal operating conditions. The power interruption circuit also is coupled to the fault detection circuit, and configured to decouple the plurality of line terminals from the plurality of load terminals in response to the fault detection circuit detecting the at least one fault. A test circuit is coupled to the fault detection circuit and the power interruption circuit.

Abstract: The present invention is directed to a protective device that includes a plurality of line terminals configured to be connected to an electrical distribution system, and a plurality of load terminals configured to be connected to at least one load. The device includes a fault detection circuit coupled to the plurality of line terminals and the plurality of load terminals. The fault detection circuit is configured to detect at least one fault condition. A power interruption circuit coupled to the fault detection circuit, the power interruption circuit including a set of movable contacts configured to decouple the plurality of line terminals from the plurality of load terminals in response to the fault detection circuit detecting the at least one fault condition. A reset mechanism is coupled to the power interruption circuit and configured to actuate the movable contacts to re-couple the plurality of line terminals to the plurality of load terminals. A lock-out mechanism is coupled to the reset mechanism.

Abstract: The invention relates to the field of land mines and in particular to devices and techniques for neutralising and/or retrieving land mines. Existing mine retrieval/neutralisation techniques include mechanical excavation or shaped charge attack and such techniques are either susceptible to blast damage in the event of inadvertent mine explosion or can produce ambiguous results (i.e. an operator can be unsure as to whether a mine has been successfully attacked or a non-mine target has been attacked in error). The present invention provides a mine retrieval technique that involves spearing the mine for later retrieval to the surface.

Abstract: A protective device includes a test button for enabling a test signal for testing an operating condition of at least one of the device components, such as the sensor, detector, switch, solenoid and trip mechanism. The test button also enables a current through a resistor body which is affixed to a stationary part of the device. The resistor body keeps a lockout spring under tension. Failure of the test signal to operate the trip mechanism within a predetermined time interval causes the resistor body conducting said current to reach a predetermined temperature, wherein the resistor body ceases to hold a lockout spring, thereby permitting the lockout spring to move to a position which causes the set of interrupting contacts to remain permanently in a disconnected position.