Abstract: A method of processing data comprises receiving, at a processor, first data comprising a plurality of values; receiving second data comprising one or more directives and association information; retrieving a first set of records from the first data using a first subset of the second data; generating a second set of records from the second data using a second subset of the second data; generating, based on a third subset of the second data, a plurality of records associations, the plurality of records associations including one or more of: first relationships between records of the first set of records, second relationships between records of the second set of records, and third relationships between records of the first set of records and the second set of records; storing the plurality of records associations in a structured format; and generating feedback data based on the plurality of records associations.

Abstract: A method of processing data comprises receiving, at a processor, first data comprising a plurality of values; receiving second data comprising one or more directives and association information; retrieving a first set of records from the first data using a first subset of the second data; generating a second set of records from the second data using a second subset of the second data; generating, based on a third subset of the second data, a plurality of records associations, the plurality of records associations including one or more of: first relationships between records of the first set of records, second relationships between records of the second set of records, and third relationships between records of the first set of records and the second set of records; storing the plurality of records associations in a structured format; and generating feedback data based on the plurality of records associations.

Abstract: A method of processing data comprises receiving, at a processor, first data comprising a plurality of values; receiving second data comprising one or more directives and association information; retrieving a first set of records from the first data using a first subset of the second data; generating a second set of records from the second data using a second subset of the second data; generating, based on a third subset of the second data, a plurality of records associations, the plurality of records associations including one or more of: first relationships between records of the first set of records, second relationships between records of the second set of records, and third relationships between records of the first set of records and the second set of records; storing the plurality of records associations in a structured format; and generating feedback data based on the plurality of records associations.

Abstract: Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a sample containing multiple targets, binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample, and reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator. The methods include the steps of observing a signal from the fluorescent signal generator and applying to the sample a solution containing an oxidizing agent that substantially inactivates both the fluorescent signal generator and the enzyme. The methods further include the steps of binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample of step, reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator; and observing a signal from the fluorescent signal generator.

Abstract: Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a sample containing multiple targets, binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample, and reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator. The methods include the steps of observing a signal from the fluorescent signal generator and applying to the sample a solution containing an oxidizing agent that substantially inactivates both the fluorescent signal generator and the enzyme. The methods further include the steps of binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample of step, reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator; and observing a signal from the fluorescent signal generator.

Abstract: Methods of activating an apo-peroxidase are provided. The methods include the steps of providing a solution comprising an apo-peroxidase and a heme choline ester, hydrolyzing the heme choline ester with a choline esterase, and converting the apo-peroxidase to active peroxidase. The methods disclosed herein also provide for detecting the presence of an analyte in a sample. The methods include providing a binder capable of specifically binding the analyte wherein the binder is attached to a solid surface, applying the sample to the solid surface under conditions that permit binder-analyte binding, adding a choline esterase-conjugated binder that binds to the analyte, adding an apo-peroxidase, a heme choline ester, a peroxide, and a peroxide-activated signal generator, and detecting the signal produced by the peroxide-activated signal generator. An associated kit and components are also provided.

Abstract: Methods of activating an apo-peroxidase are provided. The methods include the steps of providing a solution comprising an apo-peroxidase and a heme choline ester, hydrolyzing the heme choline ester with a choline esterase, and converting the apo-peroxidase to active peroxidase. The methods disclosed herein also provide for detecting the presence of an analyte in a sample. The methods include providing a binder capable of specifically binding the analyte wherein the binder is attached to a solid surface, applying the sample to the solid surface under conditions that permit binder-analyte binding, adding a choline esterase-conjugated binder that binds to the analyte, adding an apo-peroxidase, a heme choline ester, a peroxide, and a peroxide-activated signal generator, and detecting the signal produced by the peroxide-activated signal generator. An associated kit and components are also provided.