Patents by Inventor Thomas Rothmann
Thomas Rothmann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230383343Abstract: Microscopy imaging that allows for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (High Density-SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ localization and identification (by in situ sequencing or sequential fluorescence hybridization) of rolonies derived from rolling circle amplification of circular oligonucleotides and in vitro sequencing of target amplified RNA or DNA in combination with SUMI identification at a subcellular level with no optical diffraction limitation in the amount of amplified target information that can be analyzed per cell. Apart from amplified RNA or DNA, the High Density-SUMI-Seq method can also be applied using linear oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.Type: ApplicationFiled: May 25, 2023Publication date: November 30, 2023Inventors: Robert Pinard, Andreas Bosio, Thomas Rothmann, Seiyu Hosono
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Publication number: 20230323438Abstract: Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (High Density—SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ localization and identification (by in situ sequencing or sequential fluorescence hybridization) of rolonies derived from rolling circle amplification of circular oligonucleotides and in vitro sequencing of target captured RNA or DNA in combination with SUMI identification at a subcellular level with no optical diffraction limitation in the amount of captured target information that can be analyzed per cell. Apart from captured RNA or DNA, the High Density—SUMI-Seq method can also be applied using linear oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.Type: ApplicationFiled: April 5, 2023Publication date: October 12, 2023Inventors: Thomas Rothmann, Robert Pinard, Seiyu Hosono, Andreas Bosio
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Publication number: 20230090206Abstract: The invention is directed to a method to obtain the spatial location and sequence information of an m-RNA target sequence on a tissue sample comprising providing a solid surface, attaching anchor molecules, binding scaffolding molecules, incorporating adenine, guanine, cytosine and thymine, incorporating thymine to the anchor molecules, removing the scaffolding, providing a tissue sample, reverser transcrining to create c-DNA, removing the c-DNA and obtaining the sequence information of the c-DNA.Type: ApplicationFiled: September 8, 2022Publication date: March 23, 2023Applicant: Miltenyi Biotec B.V. & Co. KGInventors: Thomas ROTHMANN, Robert PINARD, Hansueli MEYER, Chris Nehme
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Publication number: 20220411862Abstract: The invention is directed to a method to obtain the spatial location and sequence information of at least a part of a RNA or cDNA strand (006) in a sample comprising the steps a. hybridizing a first detection probe oligonucleotide (204) comprising 50-1000 nucleotides with its 3? and/or 5? end to the complementary part of the at least one RNA or cDNA strand, wherein the detection probe oligonucleotide is partially hybridized to a bridge oligonucleotide (205) comprising 5-100 nucleotides wherein a gap region (206) capable of binding oligonucleotides is created b. filling the gap region (206) in part with 1 to 16 barcode oligonucleotides comprising 4-20 nucleotides, wherein the barcode oligonucleotides determine the spatial information of the RNA or cDNA strand in the sample c.Type: ApplicationFiled: May 24, 2022Publication date: December 29, 2022Applicant: Miltenyi Biotec B.V. & Co. KGInventors: Heinrich SPIECKER, Sandra HALBFELD, Thomas ROTHMANN, Andreas BOSIO
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Publication number: 20220243260Abstract: Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.Type: ApplicationFiled: January 31, 2022Publication date: August 4, 2022Applicant: Miltenyi Biotec B.V. & Co. KGInventors: Thomas ROTHMANN, Robert PINARD, Hansueli MEYER, Andreas BOSIO, Heinrich SPIECKER, Seiyu HOSONO, Ryan HINDMAN, Emily NEIL, Chris NEHME
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Patent number: 9994891Abstract: The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample.Type: GrantFiled: October 11, 2012Date of Patent: June 12, 2018Assignee: QIAGEN GMBHInventors: Andy Wende, Rainer Dahlke, Ralf Himmelreich, Thomas Rothmann, Michael Eberhard, Gerd Grosshauser, Markus Jeziorski, Hans Attig, Josef Drexler, Eva Holzer
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Patent number: 9574225Abstract: The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.Type: GrantFiled: December 23, 2009Date of Patent: February 21, 2017Assignee: QIAGEN GMBHInventors: Ralf Himmelreich, Thomas Rothmann, Roland Fabis, Christoph Erbacher
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Patent number: 9422593Abstract: Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.Type: GrantFiled: May 18, 2011Date of Patent: August 23, 2016Assignee: QIAGEN GAITHRESBURG, INCInventors: Thomas Rothmann, Irina Nazarenko, Dominic O'Neil, Arvind Virmani, Brian Lowe, Shiuli Agarwal, Holly Basham
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Patent number: 9029138Abstract: The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container, the permeable container having an opening, which is pressed into the sample, then a portion of the sample brought into the permeable container in this way is separated from the rest of the sample, and the container filled with the sample in this way is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.Type: GrantFiled: August 1, 2008Date of Patent: May 12, 2015Assignee: Preanalytix GmbHInventors: Daniel Groelz, Christian Lenz, Vera Hollaender, Thomas Rothmann
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Patent number: 9005933Abstract: The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.Type: GrantFiled: August 16, 2011Date of Patent: April 14, 2015Assignee: Qiagen GmbHInventors: Christian Korfhage, Gerd Grosshauser, Thomas Rothmann, Ralf Himmelreich
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Patent number: 8937174Abstract: The invention relates to a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further processed by a microfluidic system. A container is provided with a filter and can be or is closed. A biological sample is introduced into the container. An inlet or outlet for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.Type: GrantFiled: January 13, 2009Date of Patent: January 20, 2015Assignee: Qiagen GmbHInventors: Thomas Rothmann, Ralf Himmelreich, Thomas Laue
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Patent number: 8852863Abstract: The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.Type: GrantFiled: May 4, 2010Date of Patent: October 7, 2014Assignee: Qiagen GmbHInventors: Thomas Rothmann, Holger Engel, Ralf Himmelreich, Andy Wende, Rainer Dahlke
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Publication number: 20140287955Abstract: The present invention pertains inter alia to a method for analysing a sample comprising biomolecules, which comprises the following steps: A a) lysing the sample to provide a lysed sample and optionally clearing the lysed sample; b) contacting at least a portion of the lysed sample with a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition; or B a) contacting the sample with a lysis solution thereby providing a lysis mixture; b) using the lysis mixture to reconstitute a dry composition comprising reagents for performing the analytical method, thereby providing a reconstituted composition; c) performing an analytical method using the reconstituted composition, wherein the reconstituted composition is optionally cleared and wherein subsequent to step b) at least one step is performed which supports the lysis of the sample.Type: ApplicationFiled: October 11, 2012Publication date: September 25, 2014Inventors: Andy Wende, Rainer Dahlke, Ralf Himmelreich, Thomas Rothmann, Michael Eberhard, Gerd Grosshauser, Markus Jeziorski, Hans Attig, Josef Drexler, Eva Holzer
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Patent number: 8709824Abstract: The present invention concerns a contamination barrier 5 that permits an efficient and reproducible processing of a high number of samples with the prevention of contamination of aqueous solutions 3 in open and/or automated systems, especially in the ppm range, in that it comprises at least one water immiscible hydrocarbon compound. In addition a method for the prevention of contamination during the processing of aqueous solutions 3 in open and/or automated systems is disclosed.Type: GrantFiled: January 24, 2005Date of Patent: April 29, 2014Assignee: Qiagen GmbHInventors: Thomas Rothmann, Markus Sprenger-Haussels, Petra Benkel, Sabine Hermes, Friederike Wilmer
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Patent number: 8673241Abstract: The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container with a maximum overall height of 10 mm, preferably of 5 mm, and the container filled with the sample is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.Type: GrantFiled: August 1, 2008Date of Patent: March 18, 2014Assignee: Qiagen GmbHInventors: Daniel Groelz, Christian Lenz, Vera Hollaender, Thomas Rothmann
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Patent number: 8573518Abstract: The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.Type: GrantFiled: April 24, 2009Date of Patent: November 5, 2013Assignee: QIAGEN, GmbHInventors: Thomas Rothmann, Claudia Schmid, Andreas Schafer, Gabriele Christoffel
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Publication number: 20130210019Abstract: The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.Type: ApplicationFiled: August 16, 2011Publication date: August 15, 2013Applicant: QIAGEN GMBHInventors: Christian Korfhage, Gerd Grosshauser, Thomas Rothmann, Ralf Himmelreich
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Patent number: 8361326Abstract: The present invention relates to a device and a method for treating liquids with magnetic particles, wherein at least one further central element which ensures collection and homogenization of the particles is additionally provided.Type: GrantFiled: September 19, 2008Date of Patent: January 29, 2013Assignee: Qiagen GmbHInventors: Ralf Himmelreich, Thomas Rothmann
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Patent number: 8323507Abstract: Device for the separation of magnetic particles from a liquid, comprising a first vessel (10), a second vessel (20), a connecting surface (11, 21, 30; 200) that runs from the interior of the first vessel (10) to the interior of the second vessel (20), at least one magnet (40) for the provision of a magnetic field, and a guide element (50) by means of which the magnetic field can be guided along one side of the connecting surface (11, 21, 30; 200).Type: GrantFiled: August 18, 2006Date of Patent: December 4, 2012Assignee: Qiagen, GmbHInventors: Thomas Rothmann, Thomas Deutschmann, Christian Lenz, Cordula (Leurs) Homberg
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Publication number: 20120149011Abstract: The invention relates to a method for detecting target nucleic acids which are detected by means of a specific sequence tag which is not part of the target nucleic acid.Type: ApplicationFiled: August 16, 2010Publication date: June 14, 2012Applicant: QIAGEN GMBHInventors: Ralf Himmelreich, Gerd Grosshauser, Thomas Rothmann