Abstract: Hyperpolarized target molecules and contrast agents comprising the same, methods of making the same, and methods of imaging using same are provided. In a further aspect, imaging performed using the hyperpolarized target molecules and contrast agent can enable real time monitoring and diagnosis of diseases including various cancers and metabolic disorders. The methods are cryogen-free and inexpensive and can be performed in a short time.

Abstract: In one aspect, the disclosure relates to precipitated hyperpolarized substrates, methods of making the same, contrast agents comprising the same, and methods of diagnosing and/or monitoring the progress of a disease using the same. In one aspect, the method comprises contacting a solution containing a first solvent and a hyperpolarized substrate with a non-polar organic solvent. In a further aspect, the precipitated hyperpolarized substrate can be separated from the first solvent, the non-polar organic solvent, or any combination thereof by filtration. In still another aspect, the method further includes redissolving the precipitated hyperpolarized substrate in a biocompatible solvent such as, for example, water or a physiologically-acceptable buffer. In any of these aspects, hyperpolarization of the substrate can be accomplished using Signal Amplification by Reversible Exchange (SABRE).

Abstract: In one aspect, the disclosure relates to a compact membrane reactor for parahydrogen induced hyperpolarization, the reactor including an inner tube constructed of a semipermeable membrane, an outer tube surrounding the inner tube, and a means for controlling the magnetic field surrounding the outer tube. In some aspects, multiple compact membrane reactors can be arranged in parallel. In other aspects, the compact membrane reactor is equipped with a mechanism for magnetic field control. Also disclosed are fluid handling systems and sample preparation systems comprising the compact membrane reactors disclosed herein. The compact membrane reactor can be operated in a batch mode, a stopped-flow mode, or a continuous flow mode and can be configured to work with existing NMR spectrometers and MRI instruments.

Abstract: A hyperpolarization system including a solution and at least one magnetic field controller. The solution includes at least parahydrogen, a polarization transfer complex (PTC), and substrate molecules. The at least one magnetic field controller is configured to apply a static ultra-low magnetic field to the solution and apply an alternating ultra-low magnetic field to the solution. Through application of the ultra-low magnetic fields to the solution, the system hyperpolarizes at least some of the substrate molecules.

Abstract: In one aspect, the disclosure relates to a method for hyperpolarizing the long-lived singlet state of 13C2 pyruvate with parahydrogen and transferring the polarization to methyl pyruvate protons for detection, or to protons in other target analytes having a long-lived hyperpolarized singlet state and protons weakly couple into the singlet spin pair. In a further aspect, the method can be conducted on conventional, proton-only MRI systems. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: Systems and methods of quantum sensing include depositing a sample volume onto an ensemble of quantum defects, hyperpolarizing spins in the sample volume, performing a sensing sequence, and reading out information regarding electronic spin states of the quantum defects in the ensemble of quantum defects, which sense the hyperpolarized spins in the sample volume.

Abstract: In one aspect, the disclosure relates to hyperpolarized target molecules and contrast agents comprising the same, methods of making the same, and methods of imaging using same. In a further aspect, imaging performed using the hyperpolarized target molecules and contrast agent can enable real time monitoring and diagnosis of diseases including various cancers and metabolic disorders. The methods are cryogen-free and inexpensive and can be performed in a short time. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

Abstract: In one aspect, the disclosure relates to a compact membrane reactor for parahydrogen induced hyperpolarization, the reactor including an inner tube constructed of a semipermeable membrane, an outer tube surrounding the inner tube, and a means for controlling the magnetic field surrounding the outer tube. In some aspects, multiple compact membrane reactors can be arranged in parallel. In other aspects, the compact membrane reactor is equipped with a mechanism for magnetic field control. Also disclosed are fluid handling systems and sample preparation systems comprising the compact membrane reactors disclosed herein. The compact membrane reactor can be operated in a batch mode, a stopped-flow mode, or a continuous flow mode and can be configured to work with existing NMR spectrometers and MRI instruments.

Abstract: Systems and methods of quantum sensing include depositing a sample volume onto an ensemble of quantum defects, hyperpolarizing spins in the sample volume, performing a sensing sequence, and reading out information regarding electronic spin states of the quantum defects in the ensemble of quantum defects, which sense the hyperpolarized spins in the sample volume.

Abstract: Provided are methods for nuclear spin polarization enhancement via signal amplification by reversible exchange at very low magnetic fields.

Abstract: Provided are methods for nuclear spin polarization enhancement via signal amplification by reversible exchange at very low magnetic fields. The spin polarization is hyperpolarization of isotopically enriched heteronuclei by using a catalyst and parahydrogen to create a complex using iridium and applying magnetic fields in the microtesia range to transfer the spin order from parahydrogen to the complex.

Abstract: Provided are methods for nuclear spin polarization enhancement via signal amplification by reversible exchange at very low magnetic fields. The spin polarization is hyperpolarization of isotopically enriched heteronuclei by using a catalyst and parahydrogen to create a complex using iridium and applying magnetic fields in the microtesia range to transfer the spin order from parahydrogen to the complex.

Abstract: Provided are methods for nuclear spin polarization enhancement via signal amplification by reversible exchange at very low magnetic fields.