Abstract: Systems and methods that allow optical detection of nanoparticles according to size and interaction with specific antibodies. Using a biological sample combined with fluorescent dyes and fluorescently labeled antibodies, implementations of the disclosed system allow for detection of >100 particles having diameters as low as 100 nm in under 10 minutes. The detection mechanisms combine confocal detection of particles in microfluidic flow devices. Concentrating the sample using hydrodynamic focusing allows detection of particles having concentrations as low as 104 particles per mL of the sample. These capabilities allow for the detection, identification, and quantitation of viruses from bodily fluids such as saliva, where biologically relevant virus concentrations of potentially infected subjects are within the range of 103-107 particles/mL.

Abstract: Systems and methods that allow optical detection of nanoparticles according to size and interaction with specific antibodies. Using abiological sample combined with fluorescent dyes and fluorescently labeled antibodies, implementations of the disclosed system allow for detection of >100 particles having diameters as low as 100 nm in under 10 minutes. The detection mechanisms combine confocal detection of particles in microfluidic flow devices. Concentrating the sample using hydrodynamic focusing allows detection of particles having concentrations as low as 104 particles per mL of the sample. These capabilities allow for the detection, identification, and quantitation of viruses from bodily fluids such as saliva, where biologically relevant virus concentrations of potentially infected subjects are within the range of 103-107 particles/mL.

Abstract: The present invention relates to a method for obtaining quantitative information on average donor-acceptor distance changes within a molecule or in between molecules using ensemble Förster resonance energy transfer (eFRET), and a measurement system comprising a controller adapted for performing the same, wherein the method comprises the steps of performing an eFRET measurement for at preferably a donor-, an acceptor-, and at least a donor-acceptor-labelled sample, wherein each sample comprises respective labelled molecule copies and wherein each eFRET measurement is performed using multiple respective labelled molecule copies, under a first and a second condition, correcting the obtained results for fluorophore-specific, condition-specific and inter-condition effects, determining condition-specific eFRET efficiencies based on the corrected results, and determining quantitative information on donor-acceptor distance changes within the molecule between the first and the second condition based on the respective