Abstract: A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective having a front lens enclosed by a surround and being designed for microscopy with an immersion liquid. In the microscope objective, the front lens and/or the surround thereof is provided with a coating which can be switched between a state which repels the immersion liquid and a state which does not repel the immersion liquid.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective being designed as an air objective for microscopy without an immersion medium or as an oil immersion objective for microscopy with an oil-based immersion medium or as a water immersion objective for microscopy with a water-based immersion medium. The front lens of the microscope objective is provided with a coating which repels an immersion medium and is lipophobic and hydrophobic if the objective is an air objective, only lipophobic if the objective is a water immersion objective, and only hydrophobic if the objective is an oil immersion objective.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective being designed as an air objective for microscopy without an immersion medium or as an oil immersion objective for microscopy with an oil-based immersion medium or as a water immersion objective for microscopy with a water-based immersion medium. The front lens of the microscope objective is provided with a coating which repels an immersion medium and is lipophobic and hydrophobic if the objective is an air objective, only lipophobic if the objective is a water immersion objective, and only hydrophobic if the objective is an oil immersion objective.

Abstract: A microscope objective for imaging a specimen using a microscope, the microscope objective having a front lens enclosed by a surround and being designed for microscopy with an immersion liquid. In the microscope objective, the front lens and/or the surround thereof is provided with a coating which can be switched between a state which repels the immersion liquid and a state which does not repel the immersion liquid.

Abstract: A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

Abstract: A procedure for the correction of spherical aberration in microscopic applications, wherein various recordings of a specimen to be observed are taken and evaluated for the purpose of changing the setting values of the optical system. The correction values are stored in a correction matrix as a function of the recording position, the recording time, the wavelength, and the temperature, wherein the determination and storage of the correction values are carried out in each recording position in the x, y, and z coordinates, and/or the correction values are determined after a selection of grid points by interpolation, so that the correction values of the interpolated correction matrix are the starting values for the subsequent exact determination by measurement.

Abstract: A method for microscope imaging of a sample, wherein a sample well is filled with liquid with the sample adhered to the bottom, illuminating and imaging the sample well from the underside and capturing at least one sample image thereof, wherein any inhomogeneity of the illumination is equalized by providing a test well with the same structure filled with liquid but no sample, making a reference measurement of the test well by illuminating the test well, imaging the illuminated test well from the underside and capturing a reference image which covers the entire bottom, analyzing the reference image to determine a brightness correction specification based on brightness fluctuation, and using the brightness correction specification to correct the sample image, including determining a position of at least a part of the sample image and using a value of the brightness correction specification assigned to the position.

Abstract: A method for terminating microscopic applications with an immersion objective, in particular for switching between the immersion objective and a dry objective or a further immersion objective, wherein after the microscopic application, the immersion objective or the microscope stage bearing a specimen and/or a specimen container is moved from the observation position. The immersion medium remaining on the specimen and/or on the specimen container is deposited at a location outside the observation position of the terminated microscopic application or a further microscopic application.

Abstract: A method for fluorescence microscopy of a sample. The method includes illuminating the sample with excitation radiation of a microscope through an illumination beam path and imaging along an optical axis to an image field on a camera chip, the illumination beam path having an adjustable diaphragm for selective illumination of a target area; selecting an area on the sample as a target area; and adjusting an adjustable diaphragm for selective illumination of the target area such that parts of the sample lying outside of the selected area are not illuminated with excitation radiation.

Abstract: A procedure for the correction of spherical aberration in microscopic applications, wherein various recordings of a specimen to be observed are taken and evaluated for the purpose of changing the setting values of the optical system. The correction values are stored in a correction matrix as a function of the recording position, the recording time, the wavelength, and the temperature, wherein the determination and storage of the correction values are carried out in each recording position in the x, y, and z coordinates, and/or the correction values are determined after a selection of grid points by interpolation, so that the correction values of the interpolated correction matrix are the starting values for the subsequent exact determination by measurement.

Abstract: An immersion medium for microscopic or macroscopic examination of an object having an index of refraction between 1.0 to 1.70, a transmission between Lambda=0.30 to 1.2 ?m, a transmission TTotal=0.8 and higher, a temperature range from 0 degrees to 50 degrees Celsius, resistance to acids/bases and heat, a shear modulus of 129 to 500 Kpa, resistance to chemicals and environmental friendliness, as well as low inherent fluorescence. The immersion medium may be configured as an elastomer immersion. Embodiments of invention can include the layout of the immersion medium in the working position of an optical system.