Abstract: The invention provides an analytical process for analysing the presence of at least one aggregated conformation prion protein in a sample of body fluid or a sample of tissue and uses the dependency of the amplification of the aggregated conformation on the shear-force intensity applied to the native conformation prion protein, which is also dependent on the specific seed present in the admixture with native conformation prion protein, for specifically analysing for the presence of an aggregated conformation prion protein in the sample.

Abstract: A shear force generating device includes a shear-force generator structured for exerting a shear force at to each volume element of a sample of body fluid or tissue and a native conformation prion protein arranged in a container. A controller controls the shear-force generator to exert a shear-force of at maximum 10% of one shear-force intensity to each volume element of the sample arranged in the container. A light source is directed into the container. An optical detector is arranged to detect light emanating from the container. The detector is coupled to a computer including code to analyze light detected by the optical detector as a measure for the content of aggregated prion protein by comparing the light detected to a Reference Information Database.

Abstract: A hydrodynamic machine comprising: a toroidal working chamber including a first bladed wheel and a second bladed wheel arranged concentrically with the first bladed wheel; and an electrohydraulic system including an open loop unit and a and closed loop control unit, a working medium accumulator, a pump operable to pump a working medium from the working medium accumulator into the toroidal working chamber, a heat exchanger, a valve operable to switch between a first position, the first position being a non-braking position, and a second position, the second position being a braking position, and a line system operable to allow the pump to pump the working medium in both the first position and the second position into the working chamber.

Abstract: The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

Abstract: A hydrodynamic machine for producing a braking torque, the hydrodynamic machine comprising: a toroidal working chamber including a first bladed wheel and a second bladed wheel arranged concentrically with the first bladed wheel; and a hydraulic system including an open loop and closed loop control unit, a working medium accumulator, a line system, a pump operable to produce a volume flow circulation of a working medium through the line system and the toroidal working chamber, a heat exchanger, and a pressure control valve operable to control or adjust the baking performance or the braking torque.

Abstract: The invention provides an analytical process for analysing the presence of at least one aggregated conformation prion protein in a sample of body fluid or a sample of tissue and uses the dependency of the amplification of the aggregated conformation on the shear-force intensity applied to the native conformation prion protein, which is also dependent on the specific seed present in the admixture with native conformation prion protein, for specifically analysing for the presence of an aggregated conformation prion protein in the sample.

Abstract: The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

Abstract: A hydrodynamic machine for producing a braking torque, the hydrodynamic machine comprising: a toroidal working chamber including a first bladed wheel and a second bladed wheel arranged concentrically with the first bladed wheel; and a hydraulic system including an open loop and closed loop control unit, a working medium accumulator, a line system, a pump operable to produce a volume flow circulation of a working medium through the line system and the toroidal working chamber, a heat exchanger, and a pressure control valve operable to control or adjust the baking performance or the braking torque.

Abstract: The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.

Abstract: The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.