Abstract: Provided are inserts (100) for preparing a cell culture chamber(s), or array of chambers, inside of histology cassettes that are suitable for three-dimensional multicellular growth of a cell or cells into spheroids, organoids, or other 3D structures, such that the resulting 3D multi-cellular structures are ready and suitable for histology processing without transfer to a different receptacle or container. Further embodiments of the invention provide methods of preparing at least one cell culture chamber using the inserts, systems for growing three-dimensional multicellular spheroids comprising culturing cells within a cell culture chamber prepared using the inserts, and systems for analyzing at least one cultured cell in vitro comprising culturing cells within a cell culture chamber prepared using the inserts.

Abstract: A conductive sleeved fastener assembly includes an electrically-conductive fastener having a fastener head and a fastener shank extending from the fastener head and an electrically-conductive fastener sleeve receiving the fastener shank of the fastener and a fastener sleeve flange provided on the fastener sleeve and disposed in direct contact with the fastener head of the fastener. A method of preparing a conductive sleeved fastener for use is also disclosed.

Abstract: A depressed center grinding wheel comprises an abrasive disc. The abrasive disc comprises a working layer, an intermediate layer, a back layer, and at least two reinforcing scrims. The working layer comprises first abrasive particles retained in a first binder material. The first abrasive particles include from 40 to 100 weight percent of first shaped abrasive particles. The back layer comprises second abrasive particles retained in a second binder material. The second abrasive particles include first crushed abrasive particles and are essentially free of shaped abrasive particles. The intermediate layer is disposed between the working layer and the back layer. The intermediate layer comprises third abrasive particles retained in a third binder material. The third abrasive particles include 25 to 75 weight percent of second shaped abrasive particles.

Abstract: A conductive fastener assembly, system, and method are described. The fastener assembly includes a nut having a counterbore formed therein. The counterbore is formed to maintain a sufficient gap between the nut and the protruding end of a fastener sleeve. The nut with the counterbore improves the conductive fastener system such that the need for a solid copper grid and a cap seal is reduced or eliminated.

Abstract: A conductive fastener assembly, system, and method, wherein the fastener assembly includes a fastener, a fastener sleeve, a nut, and a lubricant coating. The lubricant coating is deposited on a distal portion of a fastener shank and is omitted from the rest of the fastener. To overcome the stresses placed on the fastener sleeve by the insertion of the fastener shank largely devoid of a lubricant coating, the fastener sleeve is reinforced by one or more of an increased thickness, a high-strength alloy, and a soft metal coating.

Abstract: A conductive fastener assembly, system, and method are described. The fastener assembly includes a nut having a counterbore formed therein. The counterbore is formed to maintain a sufficient gap between the nut and the protruding end of a fastener sleeve. The nut with the counterbore improves the conductive fastener system such that the need for a solid copper grid and a cap seal is reduced or eliminated.

Abstract: A conductive fastener assembly, system, and method, wherein the fastener assembly includes a fastener, a fastener sleeve, a nut, and a lubricant coating. The lubricant coating is deposited on a distal portion of a fastener shank and is omitted from the rest of the fastener. To overcome the stresses placed on the fastener sleeve by the insertion of the fastener shank largely devoid of a lubricant coating, the fastener sleeve is reinforced by one or more of an increased thickness, a high-strength alloy, and a soft metal coating.

Abstract: The present disclosure describes a conductive fastener assembly, system, and method, wherein the fastener assembly comprises a nut having a counterbore formed therein. The counterbore is formed to maintain a sufficient gap between the nut and the protruding end of a fastener sleeve. The nut with the counterbore improves the conductive fastener system such that the need for a solid copper grid and a cap seal is reduced or eliminated.

Abstract: The present disclosure describes a conductive fastener assembly, system, and method, wherein the fastener assembly comprises a fastener, a fastener sleeve, a nut, and a lubricant coating. The lubricant coating is deposited on a distal portion of a fastener shank and is omitted from the rest of the fastener. To overcome the stresses placed on the fastener sleeve by the insertion of the fastener shank largely devoid of a lubricant coating, the fastener sleeve is reinforced by one or more of an increased thickness, a high-strength alloy, and a soft metal coating.

Abstract: A composite abrasive wheel comprises primary and secondary abrasive portions. The primary abrasive portion comprises shaped ceramic abrasive particles retained in a first organic binder. The secondary abrasive portion is bonded to the primary abrasive portion, and comprises secondary crushed abrasive particles retained in a second organic binder. The primary abrasive portion comprises a larger volume percentage of the shaped ceramic abrasive particles than the secondary abrasive portion. A central aperture extends through the composite abrasive wheel.

Abstract: A conductive sleeved fastener assembly includes an electrically-conductive fastener having a fastener head and a fastener shank extending from the fastener head and an electrically-conductive fastener sleeve receiving the fastener shank of the fastener and a fastener sleeve flange provided on the fastener sleeve and disposed in direct contact with the fastener head of the fastener. A method of preparing a conductive sleeved fastener for use is also disclosed.

Abstract: A conductive sleeved fastener assembly includes an electrically-conductive fastener having a fastener head and a fastener shank extending from the fastener head and an electrically-conductive fastener sleeve receiving the fastener shank of the fastener and a fastener sleeve flange provided on the fastener sleeve and disposed in direct contact with the fastener head of the fastener. A method of preparing a conductive sleeved fastener for use is also disclosed.

Abstract: A composite abrasive wheel comprises primary and secondary abrasive portions. The primary abrasive portion comprises shaped ceramic abrasive particles retained in a first organic binder. The secondary abrasive portion is bonded to the primary abrasive portion, and comprises secondary crushed abrasive particles retained in a second organic binder. The primary abrasive portion comprises a larger volume percentage of the shaped ceramic abrasive particles than the secondary abrasive portion. A central aperture extends through the composite abrasive wheel.

Abstract: A conductive sleeved fastener assembly includes an electrically-conductive fastener having a fastener head and a fastener shank extending from the fastener head and an electrically-conductive fastener sleeve receiving the fastener shank of the fastener and a fastener sleeve flange provided on the fastener sleeve and disposed in direct contact with the fastener head of the fastener. A method of preparing a conductive sleeved fastener for use is also disclosed.

Abstract: A lightning-protected conductive composite fuel tank includes a conductive material arrayed on an outer surface to at least partially cover a fastener centerline. Through holes extend along the fastener centerline through the conductive material and the underlying fuel tank shell. These through holes are countersunk into the fuel tank shell to a depth such that a fastener in a through hole will avoid electrical continuity or communication with the conductive material on the fuel tank outer surface. The gap in the countersunk through holes, between the fastener heads and the coextensive outer surface of the fuel tank, is filled with a dielectric or nonconductive material.

Abstract: A conductive sleeved fastener assembly includes an electrically-conductive fastener having a fastener head and a fastener shank extending from the fastener head and an electrically-conductive fastener sleeve receiving the fastener shank of the fastener and a fastener sleeve flange provided on the fastener sleeve and disposed in direct contact with the fastener head of the fastener. A method of preparing a conductive sleeved fastener for use is also disclosed.

Abstract: A lightning-protected conductive composite fuel tank includes a conductive material arrayed on an outer surface to at least partially cover a fastener centerline. Through holes extend along the fastener centerline through the conductive material and the underlying fuel tank shell. These through holes are countersunk into the fuel tank shell to a depth such that a fastener in a through hole will avoid electrical continuity or communication with the conductive material on the fuel tank outer surface. The gap in the countersunk through holes, between the fastener heads and the coextensive outer surface of the fuel tank, is filled with a dielectric or nonconductive material.

Abstract: A lightning-protected conductive composite fuel tank includes a conductive material arrayed on an outer surface to at least partially cover a fastener centerline. Through holes extend along the fastener centerline through the conductive material and the underlying fuel tank shell. These through holes are countersunk into the fuel tank shell to a depth such that a fastener in a through hole will avoid electrical continuity or communication with the conductive material on the fuel tank outer surface. The gap in the countersunk through holes, between the fastener heads and the coextensive outer surface of the fuel tank, is filled with a dielectric or nonconductive material.

Abstract: A lightning-protected conductive composite fuel tank includes a conductive material arrayed on an outer surface to at least partially cover a fastener centerline. Through holes extend along the fastener centerline through the conductive material and the underlying fuel tank shell. These through holes are countersunk into the fuel tank shell to a depth such that a fastener in a through hole will avoid electrical continuity or communication with the conductive material on the fuel tank outer surface. The gap in the countersunk through holes, between the fastener heads and the coextensive outer surface of the fuel tank, is filled with a dielectric or nonconductive material.