Abstract: The present disclosure relates to cytochrome P450 monooxygenases capable of oxidizing a monoterpenoid indole alkaloid (MIA) substrate and methods and uses thereof. The substrate may be a camptothecinoid, an evodiaminoid or an ellipticinoid. The disclosure further relates to method of producing hydroxylated monoterpenoid indole alkaloids, as well as derivatives and analogues of the produced hydroxylated monoterpenoid indole alkaloids. Pharmaceutical compositions comprising the hydroxylated monoterpenoid indole alkaloid derivatives are also provided.

Abstract: Parallel chromatography systems and continuous manufacturing methods are described herein that utilize two or more chromatography column skids having columns operating in parallel with automation controls governing which column to load at a given time.

Abstract: The invention comprises a bridge frame for a transfer press. In one aspect of the invention, a bridge frame includes a first plurality of horizontal beams operable to receive a press bed, a second plurality of horizontal beams disposed outwardly from the press bed and operable to receive a crown capable of moving a slide vertically relative to the press bed and the crown, and a plurality of vertical support structures disposed between the first and second plurality of horizontal beams and supporting the second plurality of horizontal beams.

Abstract: The invention comprises a bridge press, a method of assembling a bridge press, and a method of forming a work piece using a bridge press. In one aspect of the invention, a bridge press includes a first plurality of horizontal beams, a plurality of vertical support structures disposed outwardly from the first plurality of horizontal beams, and a second plurality of horizontal beams disposed outwardly from and supported by the plurality of vertical support structures. The invention further comprises a press bed disposed outwardly from the first plurality of horizontal beams and supported, at least in part, by the first plurality of horizontal beams, a crown disposed outwardly from the second plurality of horizontal beams and supported, at least in part, by the second plurality of horizontal beams, and a slide disposed between the press bed and the crown, the slide coupled to the crown and operable to move vertically relative to the bed.

Abstract: The invention comprises an apparatus for supporting one or more dies used in a transfer press. In one aspect of the invention, the press die support structure comprises a gull wing shaped press bed comprising a top portion including two opposing wing portions, at least a part of each wing portion being supportable by one of a pair of approximately parallel horizontal beams, the top portion operable to support at least one press die, and a bottom portion having a center portion disposed between the wing portions.