Abstract: Disclosed herein are improved nanozymes for targeting RNA. The disclosed nanozymes are synthesized using recombinant RNase A with site-specific cysteine-substituted mutations that can be covalently functionalized with a length-tunable multi-thiol tether and then loaded onto gold particles through multiple gold-sulfur bonds. This new RNase A loading mechanism is site specific, and it allows high-density loading of alkylthiol modified DNA oligonucleotides. The disclosed nanozymes can also include additional capturer strands and/or involve DNA-recombinant-RNase-A unibodies to further increase the nanozyme's enzymatic activity and target selectivity. Also disclosed are functional on-off switchable nanozymes to control nanozyme activity. In some embodiments, the disclosed nanozyme are core-free hollow forms.

Abstract: This disclosure relates to a lipid-based topical injection formulation containing a Long-Acting SusTained delivering lipid, a structured lipid, a polymer-conjugated lipid and an ionizable lipid, and optionally a neutral phospholipid. The lipid-based topical injection formulation enables enriched delivery of drugs at an injection site and efficient expression of proteins for a prolonged period of time. This disclosure also relates to a method of preparing the lipid-based topical injection formulation, and use of the lipid-based topical injection formulation in the delivery of biologically active substances such as nucleic acids (e.g., mRNA, miRNA, siRNA, saRNA, ASO, DNA, etc.).

Abstract: A nanoparticle preparation system, a control method of the nanoparticle preparation system, an electronic device, a non-transitory computer readable medium, and a method for preparing nanoparticles using the nanoparticle preparation system are disclosed. The nanoparticle preparation system includes a mixing element, a first liquid suction device and a second liquid suction device. The mixing element includes a plurality of mixing channels; the first liquid suction device includes a plurality of first liquid suction tubes arranged in rows along a first direction and configured to input a first preparation solution into the plurality of mixing channels through first inlets of the mixing channels, respectively; and the second liquid suction device includes a plurality of second liquid suction tubes arranged in rows along a second direction and configured to input a second preparation solution into the plurality of mixing channels through second inlets of the mixing channels, respectively.

Abstract: Aspects of the disclosure provide a method and device for unlocking communications. The method can include that, after a connection is established with a second device, a preset time interval for communications in an unlocking process is determined. The method can further include that, after the preset time interval for communications is determined, unlocking communication with the second device is performed according to the preset time interval for communications.

Abstract: A columnar storage method and a query method and system for time series data. The storage method includes: dividing a column of time series data into a plurality of pages, wherein each page stores a part of data points of the column of time series data and the sum of the data points stored in all the pages is all the data points in the column of time series data (S1); and setting two parts, i.e., a page header and a page body, for each page, storing summary index information of all the data points in the page in the page header of the page and storing data value information of all the data points in the page in the page body of the page (S2).

Abstract: Aspects of the disclosure provide a method and device for unlocking communications. The method can include that, after a connection is established with a second device, a preset time interval for communications in an unlocking process is determined. The method can further include that, after the preset time interval for communications is determined, unlocking communication with the second device is performed according to the preset time interval for communications.

Abstract: A columnar storage method and a query method and system for time series data. The storage method includes: dividing a column of time series data into a plurality of pages, wherein each page stores a part of data points of the column of time series data and the sum of the data points stored in all the pages is all the data points in the column of time series data (S1); and setting two parts, i.e., a page header and a page body, for each page, storing summary index information of all the data points in the page in the page header of the page and storing data value information of all the data points in the page in the page body of the page (S2).

Abstract: Disclosed herein are improved nanozymes for targeting RNA. The disclosed nanozymes are synthesized using recombinant RNase A with site-specific cysteine-substituted mutations that can be covalently functionalized with a length-tunable multi-thiol tether and then loaded onto gold particles through multiple gold-sulfur bonds. This new RNase A loading mechanism is site specific, and it allows high-density loading of alkylthiol modified DNA oligonucleotides. The disclosed nanozymes can also include additional capturer strands and/or involve DNA-recombinant-RNase-A unibodies to further increase the nanozyme's enzymatic activity and target selectivity. Also disclosed are functional on-off switchable nanozymes to control nanozyme activity. In some embodiments, the disclosed nanozyme are core-free hollow forms.