Abstract: A polypeptide monolayer with a high surface potential and super-hydrophilicity, and a preparation method and application thereof. The polypeptide is composed of polypeptide molecules with a molecular weight of (1.48±0.2)×105 g/mol, a height of the monolayer is 13.8-14.9 nm, the exposure of primary amino groups on the surface of the monolayer is 12-14%, a Zeta potential of the polypeptide monolayer is (?1)-5 mV; a contact angle of the monolayer is 10±1°. The monolayer serving as a surface coating material of a cardiovascular stent can be applied to treatment of cardiovascular diseases; and its super-hydrophilicity can allow a layer of hydration film to be formed on the surface of the material so as to effectively prevent protein adsorption.

Abstract: A polypeptide monolayer with a low surface potential and hydrophobicity. The polypeptide is composed of polypeptide molecules with a molecular weight of (1.48±0.2)×105 g/mol, a thickness of the monolayer is 6.2-9.0 nm, the exposure of primary amino groups on the surface of the monolayer is 9.5-15%, a Zeta potential of the polypeptide monolayer is (?3)?(?9) mV, and a contact angle of the monolayer is (61±1°)-(84±1°). The monolayer can be ultrathin, with a minimum thickness of only about 6.6 nm. The polypeptide monolayer can also be applied to the preparation of a biosensor, which is conductive to increase in limit of detection. The content of primary amino groups on the surface of polypeptide monolayer is conductive to controllability of further chemical modification and laying the foundation for achieving controllable grafting of polysiloxane and biological preparations in the later stage.

Abstract: A polypeptide monolayer with a high surface potential and hydrophobicity, and a preparation method and application thereof. The polypeptide is composed of polypeptide molecules with a molecular weight of (1.48±0.2)×105 g/mol, a thickness of the monolayer is 17.3-18.5 nm, the exposure of primary amino groups on the surface of the monolayer is 11-11.8%, a Zeta potential of the polypeptide monolayer is (-3)-(-2) mV, and a contact angle of the monolayer is 84±1°. A micro-nano structure on the surface of the polypeptide monolayer allows the polypeptide monolayer to have certain hydrophobicity, which is beneficial to water proofing of the surface of biomimetic skin. Furthermore, the surface of the polypeptide monolayer has a relatively high potential, which can improve biocompatibility, hemocompatibility, and cell adhesion, proliferation, and differentiation abilities.