Abstract: A liquid yeast one- and two-hybrid high-throughput library screening method and use thereof changes the screening of solid medium to liquid culture solution screening, avoiding a large number of plate coating work, and avoiding the tedious work of identifying single colonies one by one, which not only saves experiment time, but also reduces the cost and workload; at the same time, the present invention also combines high-throughput sequencing technology to improve the analysis throughput of yeast hybridization screening work, and can also improve the automation level of analysis, and can greatly reduce false negatives. More importantly, the present invention can solve the phenomenon of protein self-activation. By changing to liquid screening, known protein interaction information can be successfully obtained, which provides a new opportunity for protein-protein interaction research.

Abstract: The present invention discloses a plant fertility-associated protein and its application. The present invention claims a method for obtaining male-sterile plants by reducing or inhibiting the activity and/or content of GmMS1 protein in the target plants. The present invention also claims a method for obtaining male-sterile plants by silencing or suppressing the expression of GmMS1 gene or knocking out the GmMS1 gene in the target plants. The present invention is of great significance to the research of plant fertility and the breeding of sterile plants.