Abstract: The disclosure generally relates to methods, cells, and compositions for preparing cardiac extracellular matrix. In particular, provided herein are methods for preparing a cardiac extracellular matrix using SUSD2 High fibroblasts and SUSD2 High myofibroblasts.

Abstract: An integrally assembled hidden beam hollow two-way floor system includes precast slabs (1), honeycomb steel beams (3), and cast-in-place concrete (4). A plurality of compartments (12) arranged in a matrix is integrally provided on one surface of each of the precast slabs (1), and connecting grooves (14) are provided at a periphery of another surface of the precast slab (1) is provided with connecting grooves (14). Prepared bolt holes (13) penetrating through the precast slab (1) are provided in the connecting grooves (14). The honeycomb steel beam (3) serves as a primary beam or a secondary beam of a steel structure of the floor system. A surface of the precast slab (1) provided with no compartments is lapped and fixed between the secondary beams or between the primary beam and the secondary beam, and adjacent precast slabs (1) are connected and fixed by a U-shaped hoop (2) passing through the prepared bolt hole (13) to form a seam.

Abstract: An integrally assembled hidden beam hollow two-way floor system includes precast slabs (1), honeycomb steel beams (3), and cast-in-place concrete (4). A plurality of compartments (12) arranged in a matrix is integrally provided on one surface of each of the precast slabs (1), and connecting grooves (14) are provided at a periphery of another surface of the precast slab (1) is provided with connecting grooves (14). Prepared bolt holes (13) penetrating through the precast slab (1) are provided in the connecting grooves (14). The honeycomb steel beam (3) serves as a primary beam or a secondary beam of a steel structure of the floor system. A surface of the precast slab (1) provided with no compartments is lapped and fixed between the secondary beams or between the primary beam and the secondary beam, and adjacent precast slabs (1) are connected and fixed by a U-shaped hoop (2) passing through the prepared bolt hole (13) to form a seam.

Abstract: The present disclosure discloses the Twa1 gene and its expression products and applications. This disclosure first discovers and verifies the role of the Twa1 gene and its expression product in the occurrence and development of cancer. The Twa1 gene and its expression product has the ability to promote cell proliferation, growth, migration, invasion, and tumor formation, thereby providing a diagnosis of malignant tumors. This disclosure also provides new targets and strategies for inhibiting or interfering with the occurrence and development of many malignant tumors. The present disclosure provides an expression vector containing the Twa1 gene and a transgenic cell line and host bacteria containing the expression vector.