Abstract: A fusion protein comprises a nanocage monomer; and an antibody or fragment thereof linked to the nanocage monomer, the antibody or fragment thereof comprising a first member of a binding pair; wherein a plurality of the fusion proteins self-assemble to form a nanocage in which a plurality of the antibodies or fragments thereof decorate the exterior surface of the nanocage, whereby the first member of the binding pair is exposed for interacting with a second member of a binding pair.

Abstract: A fusion protein comprises a nanocage monomer; and an antibody or fragment thereof linked to the nanocage monomer, the antibody or fragment thereof comprising a first member of a binding pair; wherein a plurality of the fusion proteins self-assemble to form a nanocage in which a plurality of the antibodies or fragments thereof decorate the exterior surface of the nanocage, whereby the first member of the binding pair is exposed for interacting with a second member of a binding pair.

Abstract: The present disclosure belongs to the technical field of health food, and in particular, relates to the use of PAYCS in the regulation of intestinal flora, metabolites, and brain neurotransmitters. PAYCS remedies scopolamine-induced memory impairment in mice through target oxidation, inflammatory stress and regulation of the intestinal microorganism-metabolite-brain neurotransmitter axis through PAYCS. PAYCS improves the memory pathway through the intestinal microorganism-metabolite-neurotransmitter axis. PAYCS can significantly reduce serum MDA and LDH levels and significantly increase liver SOD content. PAYCS-H inhibits the increase of liver TNF-? and IL-1?, while PAYCS-L can only reduce the content of TNF-? in the liver. Thus, PAYCS-L changes the ratio of Bacteroides/Firmicutes and increases the relative abundance of plants such as Cactaceae and Prevotellaceae, improving the neurotransmitters associated with the metabolism of tryptophan in the brain.

Abstract: The present invention discloses an anti-aging salmon roe tripeptide, having a sequence of YLP. The present invention further discloses a preparation method of the anti-aging salmon roe tripeptide, specifically including the following steps: (1) adding deionized water to salmon roe for homogenization to obtain a salmon roe homogenate; (2) regulating a pH value of the salmon roe homogenate, and adding pancreatin for enzymolysis, enzyme inactivation and cooling to obtain a salmon roe enzymatic hydrolysate; and (3) centrifuging the salmon roe enzymatic hydrolysate, taking a supernatant, and storing the supernatant for later use. A salmon roe peptide prepared by the present invention can increase levels of oxidative stress and skin-related factors (type I collagen and hyaluronic acid) by regulating a cell proliferation state, thereby achieving the protective effect on skin cells on the cell level.

Abstract: The present invention discloses a circulating multidimensional liquid chromatography and application thereof. The liquid chromatography comprises an infusion device, a sampling device, a collecting-resampling device, a chromatographic column switching device and a detecting device which are connected sequentially, wherein the collecting-resampling device comprises a collecting valve and a sample ring, and the collecting valve is connected with the sample ring; the sampling device is successively connected with the collecting valve and the chromatographic column switching device; the detecting device is also connected with the collecting valve; and the collecting valve is a three-slot three-position multi-way valve.

Abstract: The present invention discloses a circulating multidimensional liquid chromatography and application thereof. The liquid chromatography comprises an infusion device, a sampling device, a collecting-resampling device, a chromatographic column switching device and a detecting device which are connected sequentially, wherein the collecting-resampling device comprises a collecting valve and a sample ring, and the collecting valve is connected with the sample ring; the sampling device is successively connected with the collecting valve and the chromatographic column switching device; the detecting device is also connected with the collecting valve; and the collecting valve is a three-slot three-position multi-way valve.

Abstract: The present disclosure belongs to the technical field of health food, and in particular, relates to the use of PAYCS in the regulation of intestinal flora, metabolites, and brain neurotransmitters. PAYCS remedies scopolamine-induced memory impairment in mice through target oxidation, inflammatory stress and regulation of the intestinal microorganism-metabolite-brain neurotransmitter axis through PAYCS. PAYCS improves the memory pathway through the intestinal microorganism-metabolite-neurotransmitter axis. PAYCS can significantly reduce serum MDA and LDH levels and significantly increase liver SOD content. PAYCS-H inhibits the increase of liver TNF-? and IL-1?, while PAYCS-L can only reduce the content of TNF-? in the liver. Thus, PAYCS-L changes the ratio of Bacteroides/Firmicutes and increases the relative abundance of plants such as Cactaceae and Prevotellaceae, improving the neurotransmitters associated with the metabolism of tryptophan in the brain.

Abstract: A fusion protein comprises a first nanocage monomer subunit of a nanocage monomer; and a bioactive moiety linked to the first nanocage monomer subunit; wherein the fusion protein self-assembles with a protein comprising a second nanocage monomer subunit to form a nanocage monomer.

Abstract: A fusion protein comprises a nanocage monomer; and an antibody or fragment thereof linked to the nanocage monomer, the antibody or fragment thereof comprising a first member of a binding pair; wherein a plurality of the fusion proteins self-assemble to form a nanocage in which a plurality of the antibodies or fragments thereof decorate the exterior surface of the nanocage, whereby the first member of the binding pair is exposed for interacting with a second member of a binding pair.