Patents by Inventor Tiina Pakula
Tiina Pakula has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11371032Abstract: The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.Type: GrantFiled: June 4, 2019Date of Patent: June 28, 2022Assignee: Teknologian Tutkimuskeskus VTT OyInventors: Kaisa Marjamaa, Anu Koivula, Nina Aro, Tiina Pakula
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Publication number: 20220145278Abstract: Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Type: ApplicationFiled: November 13, 2020Publication date: May 12, 2022Inventors: MICHAEL WARD, YUN LUO, FELIPE OSEAS BENDEZU, MARI VALKONEN, MARKKU SALOHEIMO, NINA ARO, TIINA PAKULA
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Publication number: 20210284983Abstract: The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.Type: ApplicationFiled: June 4, 2019Publication date: September 16, 2021Applicant: Teknologian tutkimuskeskus VTT OyInventors: KAISA MARJAMAA, Anu KOIVULA, Nina ARO, Tiina PAKULA
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Patent number: 10876103Abstract: Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Type: GrantFiled: October 3, 2017Date of Patent: December 29, 2020Assignees: DANISCO US INC, VTT TECHNICAL RESEARCH CENTRE OF FINLAND LTDInventors: Michael Ward, Yun Luo, Felipe Oseas Bendezu, Mari Valkonen, Markku Saloheimo, Nina Aro, Tiina Pakula
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Publication number: 20190309276Abstract: Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.Type: ApplicationFiled: October 3, 2017Publication date: October 10, 2019Inventors: Michael Ward, Yun Luo, Felipe Oseas Bendezu, Mari Valkonen, Markku Saloheimo, Nina Aro, Tiina Pakula
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Patent number: 9695430Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: GrantFiled: December 21, 2015Date of Patent: July 4, 2017Assignee: Teknologian Tutkimuskeskus VTT OYInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Patent number: 9512415Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: GrantFiled: May 30, 2011Date of Patent: December 6, 2016Assignee: TEKNOLOGIAN TUTKIMUSKESKUS VTT OyInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Patent number: 9499826Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: GrantFiled: May 30, 2011Date of Patent: November 22, 2016Assignee: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Patent number: 9487791Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: GrantFiled: May 30, 2011Date of Patent: November 8, 2016Assignee: Roal OyInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20160108409Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: December 21, 2015Publication date: April 21, 2016Inventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20150307862Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 14, 2015Publication date: October 29, 2015Inventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20130244276Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 30, 2011Publication date: September 19, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20130084604Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 30, 2011Publication date: April 4, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20130078674Abstract: The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.Type: ApplicationFiled: May 30, 2011Publication date: March 28, 2013Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Tiina Pakula, Markku Saloheimo, Mari Hakkinen, Ann Westerholm-Parvinen, Merja Penttila, Marika Vitikainen
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Publication number: 20040115790Abstract: This invention relates to a promoter and to a fungal host for improved protein production. According to the invention the promoter has been modified in its response to the mechanisms mediating transcriptional down-regulation of secreted proteins under secretion stress. This invention relates also to methods for optimised protein production of secretable proteins in fungi.Type: ApplicationFiled: February 9, 2004Publication date: June 17, 2004Inventors: Tiina Pakula, Markku Saloheimo, Jaana Uusitalo, Anne Huuskonen, Adrian Watson, David Jeenes, David Archer, Marja Penttila