Patents by Inventor Tilman Achstetter

Tilman Achstetter has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Modified yeasts and uses thereof, in particular for producing steroid derivatives
    Patent number: 8173402
    Abstract: The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20?HSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: May 8, 2012
    Assignee: Aventis Pharma S.A.
    Inventors: Bruno Dumas, Gilles Cauet, Eric Degryse, Tilman Achstetter
  • Yeast strains autonomously producing steroids
    Patent number: 7977065
    Abstract: The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.
    Type: Grant
    Filed: January 7, 2010
    Date of Patent: July 12, 2011
    Assignee: Aventis Pharma S.A.
    Inventors: Roberto Spagnoli, Tilman Achstetter, Gilles Cauet, Eric Degryse, Bruno Dumas, Denis Pompon, Jacques Winter
  • MODIFIED YEASTS AND USES THEREOF, IN PARTICULAR FOR PRODUCING STEROID DERIVATIVES
    Publication number: 20110104749
    Abstract: The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20?HSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.
    Type: Application
    Filed: January 14, 2011
    Publication date: May 5, 2011
    Applicant: SANOFI-AVENTIS
    Inventors: Bruno Dumas, Gilles Cauet, Tilman Achstetter, Eric Degryse
  • Modified yeasts and uses thereof, in particular for producing steroid derivatives
    Patent number: 7879592
    Abstract: The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20?HSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.
    Type: Grant
    Filed: July 24, 2001
    Date of Patent: February 1, 2011
    Assignee: Aventis Pharma S.A.
    Inventors: Bruno Dumas, Gilles Cauet, Tilman Achstetter, Eric Degryse
  • YEAST STRAINS AUTONOMOUSLY PRODUCING STEROIDS
    Publication number: 20100112634
    Abstract: The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.
    Type: Application
    Filed: January 7, 2010
    Publication date: May 6, 2010
    Applicant: AVENTIS PHARMA S.A.
    Inventors: Roberto SPAGNOLI, Tilman ACHSTETTER, Gilles CAUET, Eric DEGRYSE, Bruno DUMAS, Denis POMPON, Jacques WINTER
  • Yeast strains autonomously producing steroids
    Patent number: 7670829
    Abstract: The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.
    Type: Grant
    Filed: January 29, 2002
    Date of Patent: March 2, 2010
    Assignee: Aventis Pharma S.A.
    Inventors: Roberto Spagnoli, Tilman Achstetter, Gilles Cauet, Eric Degryse, Bruno Dumas, Denis Pompon, Jacques Winter
  • Yeast strains autonomously producing steroids
    Publication number: 20040137556
    Abstract: The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.
    Type: Application
    Filed: February 5, 2004
    Publication date: July 15, 2004
    Inventors: Roberto Spagnoli, Tilman Achstetter, Gilles Cauet, Eric Degryse, Bruno Dumas, Denis Pompon, Jacques Winter
  • Modified yeasts and uses thereof, in particular for producing steroid derivatives
    Publication number: 20040082025
    Abstract: The present invention relates to novel yeast strains, methods and genetic constructs for their preparation, and their use for the synthesis or modification of steroidal compounds. More particularly, the invention describes strains having a reduced 20&agr;HSD type activity, in particular by modifying the GCY1 and/or YPR1 genes. The yeast strains of the invention make it possible to improve the efficiency of the synthesis or to increase the selectivity or the yields of the method, as well as the quality of the final product. The strains, methods and compounds of the invention are useful in the search for, the development and the production of products with therapeutic or prophylactic activity, in humans or animals, in particular of steroidal derivatives.
    Type: Application
    Filed: October 21, 2003
    Publication date: April 29, 2004
    Inventors: Bruno Dumas, Gilles Cauet, Tilman Achstetter, Eric Degryse
  • Yeast strains possessing the interrupted ATF2 gene and their applications
    Patent number: 6503749
    Abstract: A subject of the invention is a modified yeast strain in which the acetyl-CoA pregnenolone acetyltransferase (APAT) activity is eliminated by altering the gene coding for this activity with resultant stabilization of 3&bgr;-hydroxysteroids.
    Type: Grant
    Filed: March 1, 2001
    Date of Patent: January 7, 2003
    Assignee: Aventis Pharma S.A.
    Inventors: Tilman Achstetter, Gilles Cauet, Eric Degryse
  • Family of peptides known as xenoxins
    Publication number: 20020111299
    Abstract: The present invention relates to a peptide family designated as xenonins, wherein the representative members can be isolated from the substance secreted from the skin of Xenopus laevis, and having precious pharmacological properties. The present invention further relates to a method for preparing a peptide of the invention comprising transforming a cell with an isolated DNA fragment encoding the peptide, culturing the cell in a suitable medium and recovering the peptide of the invention from the culture.
    Type: Application
    Filed: July 23, 2001
    Publication date: August 15, 2002
    Inventors: Hanno V.J. Kolbe, Ulla B. Rasmussen, Gunther Kreil, Tilman Achstetter
  • Family of peptides known as xenoxins
    Patent number: 6277822
    Abstract: Peptide family designated as xenonins, wherein the representative members can be isolated from the substance secreted from the skin of Xenopus laevis, and having precious pharmaceutical properties.
    Type: Grant
    Filed: February 4, 2000
    Date of Patent: August 21, 2001
    Assignee: Transgene S.A.
    Inventors: Hanno V. J. Kolbe, Ulla B. Rasmussen, Günther Kreil, Tilman Achstetter
  • Yeast strains possessing the interrupted ATF2 gene and their applications
    Publication number: 20010012630
    Abstract: A subject of the invention is a modified yeast strain in which the acetyl-CoA pregnenolone acetyltransferase (APAT) activity is eliminated by altering the gene coding for this activity with resultant stabilization of 3&bgr;-hydroxysteroids.
    Type: Application
    Filed: March 1, 2001
    Publication date: August 9, 2001
    Applicant: Hoechst Marion Roussel
    Inventors: Tilman Achstetter, Gilles Cauet, Eric Degryse
  • Yeast strains possessing the interrupted ATF2 gene and their applications
    Patent number: 6218139
    Abstract: A subject of the invention is a modified yeast strain in which the acetyl-CoA pregnenolone acetyltransferase (APAT) activity is eliminated by altering the gene coding for this activity with resultant stabilization of 3&bgr;-hydroxysteroids.
    Type: Grant
    Filed: February 1, 1999
    Date of Patent: April 17, 2001
    Assignee: Hoechst Marion Roussel
    Inventors: Tilman Achstetter, Gilles Cauet, Eric Degryse
  • Family of peptides known as xenoxins
    Patent number: 6077827
    Abstract: The present invention relates to a family of peptides known as xenoxins, wherein the representative members can be isolated from the secretions from the skin of Xenopus laevis. The present invention also relates to the members of the peptide family as well as to methods for preparing them and to the DNA sequences encoding them. The invention further relates to pharmaceutical compositions containing said xenoxins as well as to diagnostic kits containing xenoxins or containing anti-xenoxin antibodies.
    Type: Grant
    Filed: December 28, 1995
    Date of Patent: June 20, 2000
    Assignee: TRANSGENE, S.A.
    Inventors: Hanno V. J. Kolbe, Ulla B. Rasmussen, Gunther Kreil, Tilman Achstetter
  • Promoters for expressing proteins of interest in yeast
    Patent number: 5952195
    Abstract: The present invention provides novel nucleic acid fragments isolated from the genomic DNA of yeast and having transcription promoter activity, as well as expression cassettes, vectors, and host cells containing said nucleic acid fragments. The present invention also provides methods for incorporating the nucleic acid fragments of the present invention for producing commercially or therapeutically useful polypeptides.
    Type: Grant
    Filed: March 9, 1998
    Date of Patent: September 14, 1999
    Assignee: Transgene S.A.
    Inventors: Valerie Nacken, Tilman Achstetter
  • Yeast strains for the production of mature heterologous proteins, especially hirudin
    Patent number: 5879926
    Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 9, 1999
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter
  • Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins
    Patent number: 5631144
    Abstract: The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.
    Type: Grant
    Filed: February 7, 1995
    Date of Patent: May 20, 1997
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter, Jean-Marc Reichhart
  • Yeast vector coding for heterologous gene fusions linked via KEX2 cleavage site and coding for truncated KEX2 genes
    Patent number: 5521093
    Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.
    Type: Grant
    Filed: February 23, 1995
    Date of Patent: May 28, 1996
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter
  • Saccharomyces cerevisiae strain productive of a heterologous protein and process for preparing the said heterologous protein by fermentation of the said strain
    Patent number: 5162208
    Abstract: The invention relates to a new strain of Saccharomyces cerevisiae productive of a heterologous protein, and more especially of hirudin, characterized by a suppression of the proteolytic function encoded by the PRC1 gene. More specifically, this deficiency is due to a mutation or more especially to a deletion of the PRC1 structural gene.
    Type: Grant
    Filed: March 29, 1990
    Date of Patent: November 10, 1992
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter