Abstract: The present invention concerns an enzymatic process for the preparation of a triglyceride ingredient comprising triglycerides present in human breast milk.

Abstract: The present invention concerns an enzymatic process for the preparation of an ingredient comprising 1,3-Olein-2-palmitin (OPO), the most abundant triglyceride present in human breast milk. This is achieved by using immobilized lipase from Ther-momyces lanuginosis for producing 1,3-olein-2-palmitin (1,3-Dioleate-2-palmitate-glycerol) using as substrate tripalmitin or triglycerides enriched in palmitic acid at SN-2 position by first alcoholysis in presence of C3 to C5 alcohol (butanol, pentanol, isopropanol) to produce 2-monopalmitin which is purified by selective crystallization at decreased temperature, followed by esterification using the same lipase and oleic acid.