Patents by Inventor Tim Fox
Tim Fox has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11926823Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.Type: GrantFiled: November 11, 2021Date of Patent: March 12, 2024Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Andrew Mark Cigan, Tim Fox, Manjit Singh
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Publication number: 20230203516Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.Type: ApplicationFiled: August 8, 2022Publication date: June 29, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: TIM FOX, WILLIAM JAMES GORDON-KAMM, RACHEL CAROL HUEGEL, KEITH S. LOWE, JON AARON TUCKER REINDERS, HUAXUN YE
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Publication number: 20230132042Abstract: Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.Type: ApplicationFiled: September 29, 2022Publication date: April 27, 2023Inventors: SHAI J. LAWIT, Marc C. Albertsen, Tim Fox, William Gordon-Kamm, Michelle M. Van Allen, Jean-Philippe Vielle Calzada, Carlos Barragan Rosillo, Edgar Demesa Arevalo, Carlos Gonzalez Chavez, Elvira Hernandez Lagana, Gloria Leon Martinez, Nidia Sanchez Leon, Daniel Rodriguez Leal, Isaac Rodriguez Arevalo, Jaime Padilla Calzada
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Publication number: 20230102527Abstract: The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize maintainer plants. Also provided are assays for detecting the presence of the maize DP-056113-9 event based on the DNA sequence of the recombinant DNA construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: January 26, 2021Publication date: March 30, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: HEATHER MARIE CHRISTENSEN, SARAH COLLINSON, TIM FOX, KRISTIN HAUG COLLET, MARY TRIMNELL
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Patent number: 11466288Abstract: Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.Type: GrantFiled: September 21, 2015Date of Patent: October 11, 2022Assignees: Pioneer Hi-Bred International, Inc.Inventors: Shai J. Lawit, Marc C. Albertsen, Tim Fox, William Gordon-Kamm, Michelle M. Van Allen, Jean-Philippe Vielle Calzada, Carlos Barragan Rosillo, Edgar Demesa Arevalo, Carlos Gonzalez Chavez, Elvira Hernandez Lagana, Gloria Leon Martinez, Nidia Sanchez Leon, Daniel Rodriguez Leal, Isaac Rodriguez Arevalo, Jaime Padilla Calzada
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Patent number: 11447786Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.Type: GrantFiled: October 12, 2018Date of Patent: September 20, 2022Inventors: Tim Fox, William James Gordon-Kamm, Rachel Carol Huegel, Keith S Lowe, Jon Aaron Tucker Reinders, Huaxun Ye
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Publication number: 20220275383Abstract: Isolated polynucleotides and polypeptides, and recombinant DNA constructs useful for regulating plant sterility are provided, and methods utilizing these recombinant DNA constructs are also provided. Compositions comprising these recombinant DNA constructs or a modified endogenous sterile gene are further provided.Type: ApplicationFiled: July 5, 2019Publication date: September 1, 2022Applicants: SINOBIOWAY BIO-AGRICULTURE GROUP CO. LTD., PIONEER OVERSEAS CORPORATIONInventors: GUIHUA LU, GUANFAN MAO, GUOKUI WANG, WEI WANG, HUANHUAN DING, YUZHEN ZHENG, MARC C ALBERTSEN, TIM FOX
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Publication number: 20220056439Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.Type: ApplicationFiled: November 11, 2021Publication date: February 24, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, TIM FOX, MANJIT SINGH
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Publication number: 20220056472Abstract: Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.Type: ApplicationFiled: September 13, 2019Publication date: February 24, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: MARC C ALBERTSEN, TIM FOX, MANJIT SINGH, MARK E WILLIAMS
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Publication number: 20210139927Abstract: A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.Type: ApplicationFiled: November 20, 2020Publication date: May 13, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: MARC C ALBERTSEN, TIM FOX, APRIL L LEONARD, BAILIN LI, BRIAN R LOVELAND, MARY TRIMNELL
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Patent number: 10870860Abstract: A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.Type: GrantFiled: November 19, 2018Date of Patent: December 22, 2020Inventors: Marc C Albertsen, Tim Fox, April L Leonard, Bailin Li, Brian R Loveland, Mary Trimnell
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Publication number: 20200263189Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.Type: ApplicationFiled: October 12, 2018Publication date: August 20, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: TIM FOX, WILLIAM JAMES GORDON-KAMM, RACHEL CAROL HUEGEL, KEITH S LOWE, JON AARON TUCKER REINDERS, HUAXUN YE
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Patent number: 10548276Abstract: Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.Type: GrantFiled: August 30, 2017Date of Patent: February 4, 2020Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Tim Fox, Dale F. Loussaert, Jeffrey Schussler, Juan Pablo San Martin Toloza, Bo Shen
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Publication number: 20190211354Abstract: A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.Type: ApplicationFiled: November 19, 2018Publication date: July 11, 2019Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: MARC C. ALBERTSEN, TIM FOX, APRIL L. LEONARD, BAILIN LI, BRIAN R. LOVELAND, MARY TRIMNELL
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Publication number: 20190177722Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.Type: ApplicationFiled: December 10, 2018Publication date: June 13, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, TIM FOX, MANJIT SINGH
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Publication number: 20190024102Abstract: Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.Type: ApplicationFiled: August 30, 2017Publication date: January 24, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: TIM FOX, DALE F. LOUSSAERT, JEFFREY SCHUSSLER, JUAN PABLO SAN MARTIN TOLOZA, BO SHEN
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Patent number: 10155961Abstract: A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.Type: GrantFiled: March 12, 2013Date of Patent: December 18, 2018Assignees: PIONEER HI-BRED INTERNATIONAL. INC., E I DU PONT DE NEMOURS AND COMPANYInventors: Marc Albertsen, Tim Fox, April Leonard, Bailin Li, Brian Loveland, Mary Trimnell
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Publication number: 20180258445Abstract: Nucleotide sequences, amino acid sequences, and an associated promoter sequence are useful in methods of controlling male fertility in plants. Recombinant expression cassettes, vectors, plant cells, and plants comprise a disclosed nucleotide sequence and may encode a disclosed amino acid sequence. The recombinant expression cassettes are useful in controlling fertility, especially male fertility of annual crops. The promoter is useful in driving expression of an operably-linked heterologous polynucleotide. Constructs comprising homology to the promoter sequence are useful for downregulation of a polynucleotide associated with the promoter.Type: ApplicationFiled: May 14, 2018Publication date: September 13, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: MARC C ALBERTSEN, TIM FOX, APRIL LEONARD, BAILIN LI, BRIAN LOVELAND, MARY TRIMNELL
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Publication number: 20170342430Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.Type: ApplicationFiled: August 10, 2017Publication date: November 30, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, TIM FOX, HOWARD P HERSHEY, ERICA UNGER-WALLACE, YONGZHONG WU
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Patent number: 9803215Abstract: Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.Type: GrantFiled: November 17, 2014Date of Patent: October 31, 2017Assignees: PIONEER HI-BRED INTERNATIONAL, INC., E I DU PONT DE NEMOURS AND COMPANYInventors: Marc Albertsen, Tim Fox, Mary Trimnell, Yongzhong Wu, Keith Lowe