Abstract: A novel fixation method is presented that will circumvent both biological and safety concerns with current fixation methods.

Abstract: Certain embodiments are directed to methods of measuring single cell levels of biomarkers associated with prostate cancer.

Abstract: Certain embodiments are directed to methods of measuring single cell levels of biomarkers associated with prostate cancer.

Abstract: Disclosed herein is a method for treating a subject having or suspected of having a disease or condition associated with aberrant hypomethylation of one or more genes in the subject, the method involving a tailored epigenomic modification of the subject using methylated polynucleotides directed to hypomethylated genes in the subject. Also disclosed herein is a medicinal composition for treating a subject having or suspected of having a disease or condition associated with aberrant hypomethylation of one or more genes in the subject, the composition including methylated polynucleotides directed to hypomethylated genes in the subject, so as to result in a tailored epigenomic modification of the subject upon administration.

Abstract: A method of analyzing circulating tumor cells includes separating circulating tumor cells from a subject's blood sample by filtration; separating CD45-negative cells from any retained blood cells; and determining the EMT-related gene expression profiles, nanomechanical and/or nanochemical properties of the collected CD45-negative CTC cells. Analysis of the collected circulating tumor cells enables the investigators to make a quick and accurate assessment of the metastatic behavior of the subject's circulating tumor cells for early castration-resistance and metastasis prediction in a clinical setting.

Abstract: Disclosed herein is a method to determine the biological function(s) of a target gene in a cell, the steps of which involve separately culturing a first population and a second population of the cell under same culturing conditions, the first population of the cell differing from the second population of the cell in that the first population of the cell has accepted the introduction of a methylated polynucleotide; comparing the first population and the second population of the cell to determine which biological difference(s) is/are present therebetween; and determining which biological function(s) the target gene is associated with based on the determined biological difference(s) between the first population and the second population of the cell.

Abstract: Disclosed herein is a three-component gene expression reporting system for mammalian cells, the system including a first expression cassette, a second expression cassette, and a methylated polynucleotide. The three-component gene expression reporting system can be used to establish recombinant mammalian cells for use in the screening of a demethylating agent.

Abstract: The present invention provides a method of hybridization, differential methylation hybridization (DMH) for high throughput methylation analysis of multiple CpG island loci. DMH utilizes nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.

Abstract: Disclosed herein is a method for treating a subject having or suspected of having a disease or condition associated with aberrant hypomethylation of one or more genes in the subject, the method involving a tailored epigenomic modification of the subject using methylated polynucleotides directed to hypomethylated genes in the subject. Also disclosed herein is a medicinal composition for treating a subject having or suspected of having a disease or condition associated with aberrant hypomethylation of one or more genes in the subject, the composition including methylated polynucleotides directed to hypomethylated genes in the subject, so as to result in a tailored epigenomic modification of the subject upon administration.

Abstract: Disclosed herein is a method to determine the biological function(s) of a target gene in a cell, the steps of which involve separately culturing a first population and a second population of the cell under same culturing conditions, the first population of the cell differing from the second population of the cell in that the first population of the cell has accepted the introduction of a methylated polynucleotide; comparing the first population and the second population of the cell to determine which biological difference(s) is/are present therebetween; and determining which biological function(s) the target gene is associated with based on the determined biological difference(s) between the first population and the second population of the cell.

Abstract: Novel methods are herein provided for high-throughput, dual analysis of DNA methylation and gene expression, and triple analysis of DNA methylation, gene expression and gene-associated histone acetylation in cancer cells using arrayed expressed CpG island sequence tags (ECISTs). ECISTs correspond to genomic DNA fragments comprising GC-rich segments along with promoter and/or exon (e.g., first exon) portions of genes. The GC-rich segments are useful for screening hypermethylated CpG sites in cancer cells, while the corresponding promoter and exon-containing portions are useful for determining corresponding transcript levels and assessing histone acetylation. Also provided are high-throughput methods for either confirming methylation-dependent gene silencing, or identifying therapeutically effective demethylating agents, using the ECIST array panels to identify hypermethylated loci, and measure expression levels thereof after cellular exposure to demethylating agents.

Abstract: The present invention provides a method of hybridization, differential methylation hybridization (DMH) for high throughput methylation analysis of multiple CpG island loci. DMH utilizes nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.

Abstract: The present invention provides a method of hybridization, differential methylation hybridization (DMH) for high throughput methylation analysis of multiple CpG island loci. DMH utilizes nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.

Abstract: The present invention provides a method of hybridization, differential methylation hybridization (DMH) for high throughput methylation analysis of multiple CpG island loci. DMH utilizes nucleic acid probes prepared from a cell sample to screen numerous CpG dinucleotide rich fragments affixed on a screening array. Positive hybridization signals indicate the presence of methylated sites. Methods of preparing the hybridization probes and screening array are also provided.