Abstract: A container or dish is useful for the micro manipulation, micro injection, biopsy and fertilization in oocyte and embryo manipulation and culturing. The dish allows the user to more readily perform procedures used to fertilize oocytes by Intracytoplasmic sperm injection (ICSI) or other procedures, biopsy embryos and perform additional procedures for use in assisted reproductive techniques (ART), human reproduction and in-vitro fertilization (IVF). The invention allows ease in use, the reduction in the number of micro tools used in the procedure, as opposed to conventional dishes and procedures, and will allow the user to more readily locate the oocytes and embryos to be handled and worked on. The invention will add repetitiveness, consistency, improve efficacy and ease of procedure and may improve outcomes. The invention will also give the user a more ergonomically practical dish for these types of procedures and related protocols.

Abstract: A biological specimen processing dish assembly includes a dish portion for use in treating specimens such as oocytes, embryos, tissues, and the like sequientially with different reagents or media. The dish includes a plurality of geometrically arranged wells disposed in related sets. The wells are operative to receive reagents or media for the use in treatment of the specimens in question. The specimens can be subjected to a sequence of different treatment media in the wells in a set thereof so as to provide a coherant device for subjecting specimens to a treatment procedure that requires the specimens to be subjected to different media or reagents during sequencial steps in the treatment protocol. The dish can be partially filled with a layer of oil that overlies the reagents in the wells and protects the specimens from exposure to air during the treatment protocol.