Abstract: A device (1) for sensing an analyte, the device (1) comprises at least a sample inlet (10) for receiving a sample, affinity probes (111) selected to have a preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte, the transducer not being a FET transducer, and a desalting unit (13) for desalting the received sample.

Abstract: The present disclosure provides a system for cell engineering target cells in a mixture that includes the target cells and non-target cells, the system including: i) a cell monitoring component for tracking and classifying individual cells in the mixture, wherein classifying a cell comprises determining whether the cell is a target cell; ii) an engineering component for selectively modifying individual cells at one or more engineering sites, wherein the target cells and the non-target cells are randomly distributed in the engineering component; and iii) a control component for controlling the engineering component so as to selectively modify only target cells tracked to be at one of the engineering sites.

Abstract: In a one aspect, a cell engineering module is provided for cell engineering target cells in a mixture comprising the target cells and non-target cells. The cell engineering module includes: i) a cell monitoring zone for tracking and classifying individual cells in the mixture, wherein classifying a cell includes determining whether the cell is a target cell; and ii) an engineering component in the cell monitoring zone to selectively modify individual cells at one or more engineering sites, wherein the target cells and the non-target cells may be randomly distributed in the engineering component; wherein the engineering component is operable to selectively modify only target cells that have been tracked to be at one of the engineering sites.

Abstract: According to an aspect of the present inventive concept there is provided a device for characterizing objects comprising: a flow channel configured to pass a flow of liquid, wherein: the flow of liquid carries the objects, the flow channel comprises a zone associated with a category, wherein a surface is coated with molecules having an affinity and specificity to the category, whereby the surface forms an object movement modifying (OMM) surface configured to modify the movement of the object by binding and releasing the object; wherein the device is configured to modulate a force exerted on the object; wherein the device is configured to exert a first condition and a second condition on the object, the first condition being adapted to promote the binding of an object belonging to the category to the OMM surface and the second condition being adapted to promote the releasing of an object bound to the OMM surface.

Abstract: The present inventive concept relates to a method for measuring analyte concentration in a sample fluid, comprising: receiving dilution fluid or sample fluid comprising analyte in a microfluidic channel, wherein the dilution fluid or sample fluid further comprises a molecule which is different from the analyte; performing a first affinity-based assay in a first detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the dilution fluid or sample fluid; mixing the dilution fluid or sample fluid in the microfluidic channel with another of the dilution fluid or sample fluid to obtain a diluted sample fluid; performing a second affinity-based assay in a second detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the diluted sample fluid; performing a third assay in the second detection zone to measure a signal indicative of the concentration of the analyte in the diluted sample fluid; determining

Abstract: A method (100) for tracking objects (1) in a flow channel (2), comprising: receiving (S102) a time series (20) of frames (21). each frame (21) comprising observed object positions (4), the time series (20) of frames (21) comprising a first set (30) of frames (21) and a first frame (31); forming (S104) a first set (40) of tracks (41), each track (41) of the first set (40) of tracks (41) comprising observed object positions (+) from the first set (30) of frames (21); expanding (S106) the first set (40) of tracks (41) by adding observed object positions (4) of the first frame (31); detecting (S108) a track error indicating an improbable expansion of the first set (40) of tracks (41); finding (S110) a track modification, being a modification to the expanded first set (40) of tracks (41), wherein finding the track modification is based on observed object positions (4) from at least three frames (21); and modifying (S120) the expanded first set (40) of tracks (41) by the track modification.

Abstract: The disclosed technology generally relates to storing data, and more particularly relates to a method of storing data in a polymer, where the data comprises a sequence of bits. In one aspect, the method comprises receiving a sequence of bits to be stored and providing a group of different homo-bifunctional monomers. Each homo-bifunctional monomer comprises a core structure having identical functional groups attached at two different positions of the core structure. The group of different homo-bifunctional monomers comprises homo-bifunctional monomers having at least two different core structures. The method further comprises linking the different homo-bifunctional monomers together to form the polymer having a sequence of monomer core structures representing the sequence of bits to be stored. The different homo-bifunctional monomers are linked together using a click chemistry reaction between the functional groups of the different homo-bifunctional monomers.

Abstract: Example embodiments relate to microfluidics chips for performing biochemical reactions. An example microfluidics chip for performing a biochemical reaction in a reaction mixture includes at least one sample unit. The sample unit includes a plurality of cavities configured to receive the reaction mixture. Each cavity has an inlet and an outlet. The sample unit also includes a first fluid channel for transporting the reaction mixture to the plurality of cavities. The first fluid channel is fluidically connected to the inlet of each cavity. Additionally, the sample unit includes a second fluid channel for transporting oil. The second fluid channel is connected to the outlet of each cavity. The structure of the outlet of each cavity is configured to allow gas to pass from the cavity to the second fluid channel and to prevent the reaction mixture from passing from the cavity to the second fluid channel.

Abstract: According to an aspect of the present inventive concept there is provided a device for analyzing biological cells comprising: a flow channel passing a flow of liquid carrying the biological cells to be analyzed, the flow channel comprises at least one zone associated with a cell category; at least one detector to detect information representing the biological cells passing the at least one zone; a processor configured to receive and process the information by: identifying candidate biological cells; identifying tracks from the candidate biological cells; identifying candidate modified movements as events in each track; determining event related information associated with the event, the event related information comprises at least a sensed property of the candidate biological cells and/or a property defining a relation between events of at least two tracks from candidate biological cells; identifying candidate non-specific modified movements among the candidate modified movements; and removing the candidate n

Abstract: According to an aspect there is provided a biosensing device for collecting light from a sample. The device comprises a sensing site configured to hold a bioreceptor at a sensing surface, and to receive the sample on the sensing surface. The bioreceptor is configured to bind to an analyte in the sample. The sample light is emitted by or formed by scattering by the analyte bound to the sensing surface. The device comprises a light guiding layer with first surface facing the sensing surface or forming the sensing surface, and having a second surface, opposite to the first surface. The layer is configured to collect supercritical angle light from the sample light of the analyte at the sensing surface, and to guide the light along an extension of the layer by total internal reflections. The layer comprises an outcoupling region for coupling the supercritical angle light through the second surface, out of the layer.

Abstract: A system for label-free identification and/or classification and/or selection of at least one cell is provided. The system comprises a microfluidic environment configured to be traversable by the at least one cell, an imaging sensor configured to gather imaging information with respect to at least a part of the microfluidic environment, and a processing unit connected to the imaging sensor. In this context, the microfluidic environment comprises at least one sub-environment configured to interact with the at least one cell. Furthermore, the processing unit is configured to detect at least one sub-environment interaction event based on persistence of the at least one cell imaged by the imaging sensor in the temporal domain. In addition to this, the processing unit is configured to identify and/or classify and/or select the at least one cell based on the at least one sub-environment interaction event.

Abstract: A method and system for electroporation of a biological cell in a fluidic channel is provided. The method includes: introducing a fluidic flow in a first direction in the fluidic channel wherein the fluidic flow comprises the cell; applying a first electrical field to the cell in the fluidic flow to produce a first electroporation at a first position in the fluidic channel; measuring an impedance of the cell in the fluidic flow at a second position in the fluidic channel; determining a second electrical field depending on the impedance measurement of the cell in the fluidic flow at the second position in the fluidic channel; and applying the second electrical field to the cell in the fluidic flow to produce a second electroporation at a third position in the fluidic channel, wherein the first, second and third positions are sequentially arranged in the first direction of the fluidic flow.

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological cells. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

Abstract: According to an aspect of the present inventive concept there is provided a microfluidic device comprising: at least one structure arranged in a pocket-defining layer defining a pocket in the pocket-defining layer; a semiconductor chip arranged in the pocket, the semiconductor chip comprising at least one electrode at the surface of the semiconductor chip; an electrical connection layer arranged above the semiconductor chip, wherein the electrical connection layer comprises electronic connections electrically connected to the at least one electrode and arranged to extend laterally in the electrical connection layer away from the semiconductor chip; at least one fluidic channel extending through the pocket-defining layer and above the semiconductor chip, the fluidic channel being arranged to be in fluidic communication with the at least one electrode.

Abstract: A method for monitoring a monitor material of a process, such as a manufacturing process, comprises: detecting a suspicious particle in a sample of the monitor material using an imaging system configured to image the sample using illumination light from a light source and to detect an interference pattern based on object light having interacted with the sample and reference light of the illumination light; selectively diverting the suspicious particle rom a flow of the sample to an analysis flow; activating a nucleic acid test (NAT) device, wherein said activating is triggered based on detecting of the suspicious particle in the sample; receiving by the NAT device the analysis flow comprising the suspicious particle; and subjecting the suspicious particle to a NAT analysis. Also, an apparatus for monitoring a monitor material is provided.

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Abstract: The present inventive concept relates to a method for measuring analyte concentration in a sample fluid, comprising: receiving dilution fluid or sample fluid comprising analyte in a microfluidic channel, wherein the dilution fluid or sample fluid further comprises a molecule which is different from the analyte; performing a first affinity-based assay in a first detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the dilution fluid or sample fluid; mixing the dilution fluid or sample fluid in the microfluidic channel with another of the dilution fluid or sample fluid to obtain a diluted sample fluid; performing a second affinity-based assay in a second detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the diluted sample fluid; performing a third assay in the second detection zone to measure a signal indicative of the concentration of the analyte in the diluted sample fluid; determining

Abstract: Example embodiments relate to microfluidic devices for controlling pneumatic microvalves. One embodiment includes a microfluidic device for independently controlling a plurality of pneumatic microvalves. The microfluidic device is couplable to a pressure source. The microfluidic device includes a first substrate. The microfluidic device also includes a flexible membrane covering the first substrate. Additionally, the microfluidic device includes a second substrate covering the flexible membrane. Further, the microfluidic device includes one or more fluidic channels at least partially defined in the first substrate. In addition, the microfluidic device includes a pressure couplable to the pressure source and branching into a plurality of pressure channels. Still further, the microfluidic device includes at least one pressure control switch per pressure channel.

Abstract: A device (1) for sensing an analyte, the device (1) comprises at least a sample inlet (10) for receiving a sample, affinity probes (111) selected to have a preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte, the transducer not being a FET transducer, and a desalting unit (13) for desalting the received sample.

Abstract: The present invention relates to a method for writing data comprising a sequence of bits, the data being written in a form of nucleic acid, by in-vitro enzymatically producing memory nucleic acid from a strand of memory writing substrate nucleic acid, wherein the strand of memory writing substrate nucleic acid comprises a plurality of spacer sections and memory writing sections sandwiched between the spacer sections. Each of the spacer sections comprises one or more nucleobases, and each of the memory writing sections comprises a nucleobase other than the nucleobases of an adjacent spacer section upstream of the memory writing section in a travel direction of an enzyme along the strand of memory writing substrate nucleic acid.