Abstract: A system for label-free identification and/or classification and/or selection of at least one cell is provided. The system comprises a microfluidic environment configured to be traversable by the at least one cell, an imaging sensor configured to gather imaging information with respect to at least a part of the microfluidic environment, and a processing unit connected to the imaging sensor. In this context, the microfluidic environment comprises at least one sub-environment configured to interact with the at least one cell. Furthermore, the processing unit is configured to detect at least one sub-environment interaction event based on persistence of the at least one cell imaged by the imaging sensor in the temporal domain. In addition to this, the processing unit is configured to identify and/or classify and/or select the at least one cell based on the at least one sub-environment interaction event.

Abstract: A method and system for electroporation of a biological cell in a fluidic channel is provided. The method includes: introducing a fluidic flow in a first direction in the fluidic channel wherein the fluidic flow comprises the cell; applying a first electrical field to the cell in the fluidic flow to produce a first electroporation at a first position in the fluidic channel; measuring an impedance of the cell in the fluidic flow at a second position in the fluidic channel; determining a second electrical field depending on the impedance measurement of the cell in the fluidic flow at the second position in the fluidic channel; and applying the second electrical field to the cell in the fluidic flow to produce a second electroporation at a third position in the fluidic channel, wherein the first, second and third positions are sequentially arranged in the first direction of the fluidic flow.

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological cells. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

Abstract: According to an aspect of the present inventive concept there is provided a microfluidic device comprising: at least one structure arranged in a pocket-defining layer defining a pocket in the pocket-defining layer; a semiconductor chip arranged in the pocket, the semiconductor chip comprising at least one electrode at the surface of the semiconductor chip; an electrical connection layer arranged above the semiconductor chip, wherein the electrical connection layer comprises electronic connections electrically connected to the at least one electrode and arranged to extend laterally in the electrical connection layer away from the semiconductor chip; at least one fluidic channel extending through the pocket-defining layer and above the semiconductor chip, the fluidic channel being arranged to be in fluidic communication with the at least one electrode.

Abstract: A method for monitoring a monitor material of a process, such as a manufacturing process, comprises: detecting a suspicious particle in a sample of the monitor material using an imaging system configured to image the sample using illumination light from a light source and to detect an interference pattern based on object light having interacted with the sample and reference light of the illumination light; selectively diverting the suspicious particle rom a flow of the sample to an analysis flow; activating a nucleic acid test (NAT) device, wherein said activating is triggered based on detecting of the suspicious particle in the sample; receiving by the NAT device the analysis flow comprising the suspicious particle; and subjecting the suspicious particle to a NAT analysis. Also, an apparatus for monitoring a monitor material is provided.

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Abstract: The present inventive concept relates to a method for measuring analyte concentration in a sample fluid, comprising: receiving dilution fluid or sample fluid comprising analyte in a microfluidic channel, wherein the dilution fluid or sample fluid further comprises a molecule which is different from the analyte; performing a first affinity-based assay in a first detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the dilution fluid or sample fluid; mixing the dilution fluid or sample fluid in the microfluidic channel with another of the dilution fluid or sample fluid to obtain a diluted sample fluid; performing a second affinity-based assay in a second detection zone of the microfluidic channel to measure a signal indicative of the concentration of the molecule in the diluted sample fluid; performing a third assay in the second detection zone to measure a signal indicative of the concentration of the analyte in the diluted sample fluid; determining

Abstract: Example embodiments relate to microfluidic devices for controlling pneumatic microvalves. One embodiment includes a microfluidic device for independently controlling a plurality of pneumatic microvalves. The microfluidic device is couplable to a pressure source. The microfluidic device includes a first substrate. The microfluidic device also includes a flexible membrane covering the first substrate. Additionally, the microfluidic device includes a second substrate covering the flexible membrane. Further, the microfluidic device includes one or more fluidic channels at least partially defined in the first substrate. In addition, the microfluidic device includes a pressure couplable to the pressure source and branching into a plurality of pressure channels. Still further, the microfluidic device includes at least one pressure control switch per pressure channel.

Abstract: A device (1) for sensing an analyte, the device (1) comprises at least a sample inlet (10) for receiving a sample, affinity probes (111) selected to have a preferential binding to the analyte, a transducer (11) sensitive to a characteristic of the analyte and/or a label attached to the analyte, the transducer not being a FET transducer, and a desalting unit (13) for desalting the received sample.

Abstract: A method for writing data including a sequence of bits, the data being written in a form of DNA, by in-vitro enzymatically producing memory DNA from a strand of memory writing substrate DNA is disclosed. In one aspect, the method includes repeating of: receiving a sub-sequence of the sequence of bits, the sub-sequence including at least one bit; selecting memory nucleotides based on the sub-sequence; contacting, in liquid medium including the strand of memory writing substrate DNA contacted with an enzyme, the selected memory nucleotides and the enzyme; and synthesizing a portion of the memory DNA from a portion of the strand of memory writing substrate DNA by the enzyme and at least one of the memory nucleotides of the solution, thereby producing memory DNA including memory nucleotides corresponding to bits of the sequence of bits. The disclosed technology further relates to a micro-fluidic system including a microfluidic chip and a controller.

Abstract: The present invention relates to a method for writing data comprising a sequence of bits, the data being written in a form of nucleic acid, by in-vitro enzymatically producing memory nucleic acid from a strand of memory writing substrate nucleic acid, wherein the strand of memory writing substrate nucleic acid comprises a plurality of spacer sections and memory writing sections sandwiched between the spacer sections. Each of the spacer sections comprises one or more nucleobases, and each of the memory writing sections comprises a nucleobase other than the nucleobases of an adjacent spacer section upstream of the memory writing section in a travel direction of an enzyme along the strand of memory writing substrate nucleic acid.

Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.

Abstract: A device for synthesis of macromolecules is disclosed. In one aspect, the device comprises an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules. The ion-releaser also includes an ion-source electrode, which is arranged to contain releasable ions and is arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby release ions to the synthesis locations. The ion-releaser further comprises activating electrodes, which are arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations via the ion-source electrode. The ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the synthesis location.

Abstract: A fluidic device (100) is described for locally coating an inner surface of a fluidic channel. The fluidic device (100) comprises a first (101), a second (102) and a third (103) fluidic channel intersecting at a common junction (105). The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device (100) further comprises a fluid control means (111) configured for creating a fluidic flow path for a coating fluid at the common junction (105) such that, when coating, a coating fluid propagates from the first (101) to the second (102) fluidic channel via the common junction (105) without propagating into the third (103) fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Abstract: The disclosure provides methods and systems for analyzing fluid samples comprising obtaining fluid samples in at least one cavity of a substrate and introducing also buffers and/or reagents in the cavity, performing nucleic acid extraction and/or purification in the cavity, and performing nucleic acid amplification in the same cavity.

Abstract: A fluidic device is described for locally coating an inner surface of a fluidic channel. The fluidic device comprises a first, a second and a third fluidic channel intersecting at a common junction. The first fluidic channel is connectable to a coating fluid reservoir and the third fluidic channel is connectable to a sample fluid reservoir. The fluidic device further comprises a fluid control means configured for creating a fluidic flow path for a coating fluid at the common junction such that, when coating, a coating fluid propagates from the first to the second fluidic channel via the common junction without propagating into the third fluidic channel. A corresponding method for coating and for sensing also has been disclosed.

Abstract: Example embodiments relate to microfluidic devices for controlling pneumatic microvalves. One embodiment includes a microfluidic device for independently controlling a plurality of pneumatic microvalves. The microfluidic device is couplable to a pressure source. The microfluidic device includes a first substrate. The microfluidic device also includes a flexible membrane covering the first substrate. Additionally, the microfluidic device includes a second substrate covering the flexible membrane. Further, the microfluidic device includes one or more fluidic channels at least partially defined in the first substrate. In addition, the microfluidic device includes a pressure couplable to the pressure source and branching into a plurality of pressure channels. Still further, the microfluidic device includes at least one pressure control switch per pressure channel.

Abstract: A sensor device for quantifying luminescent targets comprises a light source, a detector, a modulator, and a processor. The light source is adapted for exciting the luminescent target. The detector is adapted for detecting the luminescence of the luminescent target resulting in a measured signal which comprises a desired signal originating from the luminescent target and a background signal. The modulator is adapted for modulating a physical parameter resulting in a modulation of the desired signal which is different from the modulation of the background signal. The processor is configured to correlate the modulation of the physical parameter with the modulation of the desired signal and/or the modulation of the background signal.

Abstract: The present invention relates to a method for writing data comprising a sequence of bits, the data being written in a form of nucleic acid, by in-vitro enzymatically producing memory nucleic acid from a strand of memory writing substrate nucleic acid, wherein the strand of memory writing substrate nucleic acid comprises a plurality of spacer sections and memory writing sections sandwiched between the spacer sections. Each of the spacer sections comprises one or more nucleobases, and each of the memory writing sections comprises a nucleobase other than the nucleobases of an adjacent spacer section upstream of the memory writing section in a travel direction of an enzyme along the strand of memory writing substrate nucleic acid.

Abstract: A micro analysis chip comprises an inlet and a fluid flow path communicating thereto. The fluid flow path comprises a first flow path, a second flow path, and a third flow path arranged continuously along a longitudinal direction of the fluid flow path. An antibody is bound on at least one peripheral surface selected from the group consisting of peripheral surfaces of the second and third flow paths. A cross-sectional area of the third flow path is constant or increased monotonically along a direction X from the second flow path toward the third flow path. A cross-sectional area of the second flow path is increased monotonically along the direction X from the one end to the other end of the second flow path. A cross-sectional area of the first flow path is larger than a cross-sectional area at the one end of the second flow path.