Patents by Inventor Timo Gleissner
Timo Gleissner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240052311Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: October 27, 2023Publication date: February 15, 2024Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Publication number: 20240018473Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: September 22, 2023Publication date: January 18, 2024Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Patent number: 11827902Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: GrantFiled: May 24, 2022Date of Patent: November 28, 2023Assignees: LONZA WALKERSVILLE, INC., LONZA COLOGNE GMBH, OCTANE BIOTECH INC.Inventors: Yaling Shi, Erika McAfee, Samatha Bandapalle, Ann Siehoff, Timo Gleissner, Joseph O'Connor, Eytan Abraham, Kelly Purpura, Nuala Trainor, Timothy Smith
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Publication number: 20230340386Abstract: The invention relates to a device for providing a cell suspension to an apparatus for treating the cells, wherein the device comprises at least one internal space 16 for holding the suspension, and wherein the internal space 16 is at least partially surrounded by a wall 15. The device further comprises at least one inlet port 2 comprising at least one first opening 3 for introducing the suspension into the internal space 16 and at least one outlet port comprising at least one second opening for removing the suspension from said internal space 16. The inlet port 2 further comprises at least one barrier element 6 bordering the first opening 3 at a side furthest to the wall 15 and is partially arranged between the first opening 3 and the internal space 16.Type: ApplicationFiled: September 21, 2021Publication date: October 26, 2023Applicants: Lonza Cologne GmbH, Lonza Cologne GmbHInventors: Timo GLEISSNER, Claudiu TODOR
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Patent number: 11781113Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: GrantFiled: May 24, 2022Date of Patent: October 10, 2023Assignees: LONZA WALKERSVILLE, INC., LONZA COLOGNE GMBH, OCTANE BIOTECH INC.Inventors: Yaling Shi, Erika McAfee, Samatha Bandapalle, Ann Siehoff, Timo Gleissner, Joseph O'Connor, Eytan Abraham, Kelly Purpura, Nuala Trainor, Timothy Smith
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Patent number: 11661595Abstract: Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: GrantFiled: March 17, 2020Date of Patent: May 30, 2023Assignee: Lonza Cologne GmbHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20220298466Abstract: The invention relates to a method and device for controlling a filling level of a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles within at least one chamber of a device for applying an electric field to the suspension. In order to avoid overfilling of the chamber if multiple electroporation cycles are performed and to achieve exact filling in an environment of unpredictable chamber volume, the amount of suspension filled into the chamber is dynamically limited in the course of several electroporation cycles by determining at least one change of the electrical resistance at the outlet port. The resistance between at least one electrode and a grounding electrode is measured during the filling procedure of each cycle at several points in time. Once a change of resistance is detected, the termination routine is initiated and the filling procedure is finally terminated.Type: ApplicationFiled: June 28, 2020Publication date: September 22, 2022Inventors: Sven HERMSMEIER, Timo GLEISSNER
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Patent number: 11447745Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: GrantFiled: August 31, 2018Date of Patent: September 20, 2022Assignees: LONZA WALKERSVILLE, INC., LONZA COLOGNE GMBH, OCTANE BIOTECH INC.Inventors: Yaling Shi, Erika McAfee, Samatha Bandapalle, Ann Siehoff, Timo Gleissner, Joseph O'Connor, Eytan Abraham, Kelly Purpura, Nuala Trainor, Timothy Smith
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Publication number: 20220282212Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: May 24, 2022Publication date: September 8, 2022Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Publication number: 20220282213Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: May 24, 2022Publication date: September 8, 2022Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Publication number: 20220282214Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: May 24, 2022Publication date: September 8, 2022Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Patent number: 11371018Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: GrantFiled: October 30, 2019Date of Patent: June 28, 2022Assignees: OCTANE BIOTECH INC., LONZA WALKERSVILLE, INC., LONZA COLOGNE GMBHInventors: Yaling Shi, Erika McAfee, Samatha Bandapalle, Ann Siehoff, Timo Gleissner, Joseph O'Connor, Eytan Abraham, Kelly Purpura, Nuala Trainor, Timothy Smith
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Patent number: 11352615Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: GrantFiled: November 9, 2018Date of Patent: June 7, 2022Assignee: Lonza Cologne GmbHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20210324367Abstract: Disclosed is a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. Also disclosed is a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62.Type: ApplicationFiled: May 5, 2021Publication date: October 21, 2021Applicant: Lonza Cologne GmbHInventors: Thomas Koblizek, Andreas HEINZE, Timo GLEISSNER, Herbert Mueller-Hartmann, Andreas WIRTH
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Patent number: 11021698Abstract: Disclosed is a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. Also disclosed is a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62.Type: GrantFiled: May 11, 2017Date of Patent: June 1, 2021Assignee: LONZA COLOGNE GMBHInventors: Thomas Koblizek, Andreas Heinze, Timo Gleissner, Herbert Mueller-Hartmann, Andreas Wirth
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Publication number: 20200325466Abstract: Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: ApplicationFiled: March 17, 2020Publication date: October 15, 2020Applicant: Lonza Cologne GmbHInventors: Ludger ALTROGGE, Timo GLEISSNER, Andreas HEINZE, Sven HERMSMEIER
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Patent number: 10633646Abstract: The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space (40) for holding the suspension, the internal space (40) comprising at least two segments (41, 42), wherein each segment (41, 42) comprises at least one electrode (43, 44) and wherein neighboring electrodes (43, 44) are separated from each other by at least one gap (47) which is at least partially filled with an insulating material (46), and wherein the edges of the electrodes (43, 44) facing each other within the internal space (40) are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode (43, 44) while the electrodes (43, 44, 45) or electrode segments next and/or opposite to the active electrode (43, 44) are set to ground potential.Type: GrantFiled: April 28, 2015Date of Patent: April 28, 2020Assignee: LONZA COLOGNE GMBHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20200071670Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: October 30, 2019Publication date: March 5, 2020Inventors: Yaling SHI, Erika MCAFEE, Samatha BANDAPALLE, Ann SIEHOFF, Timo GLEISSNER, Joseph O'CONNOR, Eytan ABRAHAM, Kelly PURPURA, Nuala TRAINOR, Timothy SMITH
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Patent number: 10336996Abstract: The invention relates to a device (1) for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber (6) which comprises at least two electrodes (4), and at least one separating element (13) which is movable within the chamber (6) between two terminal points (14, 15) and, if it is in a position between the terminal points (14, 15), separates at least one first compartment (26) of the chamber (6) from at least one second compartment (27) of the chamber (6).Type: GrantFiled: April 28, 2015Date of Patent: July 2, 2019Assignee: LONZA COLOGNE GMBHInventors: Ludger Altrogge, Timo Gleissner, Andreas Heinze, Sven Hermsmeier
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Publication number: 20190169572Abstract: The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.Type: ApplicationFiled: August 31, 2018Publication date: June 6, 2019Inventors: Yaling SHI, Erika McAfee, Samatha Bandapalle, Ann Siehoff, Timo Gleissner, Joseph O'Connor, Eytan Abraham, Kelly Purpura, Nuala Trainor, Timothy Smith