Abstract: A fluidic device, instrument, and method for processing a biological sample is disclosed herein. An example of the fluidic device includes structures (e.g., sample holder(s), reaction chamber(s), reagent storage zone(s), metering compartment(s), reaction well(s), detection well(s), channel(s), etc.) for efficiently processing sample components and providing expression levels of various biomarker components for characterization of a sample.