Abstract: The present invention provides compositions, systems, kits, and methods for expressing at least one therapeutic protein or biologically active nucleic acid molecule in a subject. In certain embodiments, the subject is first administered a composition comprising polycationic structures that is free, or essentially free, of nucleic acid molecules, and then (e.g., 1-30 minutes later) is administered a composition comprising a plurality of one or more non-viral expression vectors that encode at least one therapeutic protein (e.g., at least one anti-SARS-CoV-2 antibody, multiple different antibodies, an ACE2 protein, or human growth hormone) or a biologically active nucleic acid molecule.

Abstract: Provided herein are compositions, systems, kits, and methods for treating a subject, and/or a subject's pre-adipocytes and/or adipocytes, with a composition containing a nucleic acid sequence encoding a protein or other biologically active nucleic acid-encoded molecule (BANEM), or a vector containing the nucleic acid sequence, wherein the treating comprises: a) injecting the composition into one or more subcutaneous (SC) regions of the subject such that one or more protein, or other BANEM, is detectable in a blood, serum, or plasma sample from the subject; and/or b) injecting the composition into one or more SC regions of the subject such that in-vivo transfected pre-adipocytes and/or adipocytes (e.g., transfected cells of fat cell origin) are generated; and/or c) performing the following: i) contacting pre-adipocytes and/or adipocytes (e.g.

Abstract: A primary master node and a standby master node monitor the health of a shared nothing database appliance to afford high availability while minimizing false negatives and duplicate alerts by executing continuously in parallel complimentary processes that determine whether the database is running, and which master node is the active database master node. The active database master node monitors the health of the components of the database appliance by polling each component to detect failures and warnings, and the other master node monitors the status of the active master node. Upon detecting a failure of the active master node, the other node takes over health monitoring. If the database is not running, the designated primary master node performs health monitoring.

Abstract: A distributed reusable framework comprising a non-logging resumable workflow of actions that runs independently in parallel to upgrade each machine of a large distributed cluster of machines. A CHECK process and a RUN process are associated with each action in the workflow. The CHECK process determines dynamically the state of each action, and the RUN process brings an action to a completed state. Following a reboot on a machine, the workflow on the machine sequences back to its start and rechecks each prior action of the workflow to verify that each action is in a completed state before. Upon a failure of the upgrade on a single machine in the cluster, the failed machine can be fixed and the workflow can be resumed without redoing the work already done. Since the actual live state is verified on each machine of the cluster, log files are unnecessary.