Abstract: A system for preventing nosocomial infection and contamination through usage of a stethoscope, utilizing a stethoscope sleeve and fitting accessory configured to be utilized in conjunction with off-the-shelf stethoscopes and the like. A first embodiment (FIGS. 1 & 2b) includes a one-use, disposable sleeve, wherein the head or diaphragm-retaining ring is covered in its entirety, with the full covering up of the body to the ear tubes, the cover being composed of a bacterial, viral, and fluid impermeable, acoustically transparent medium. The accessory includes a spreader attachment removably affixed to the upper body of the stethoscope and containable within the sleeve when mounted on the stethoscope. In combination with the spreader attachment accessory, the system provides a sterile system for preventing infection which isolates the stethoscope from both the user as well as the patient, greatly reducing any risk of contamination. A second embodiment (FIGS.