Abstract: Disclosed herein are methods of identifying a cell type by its genomic methylation pattern from a biological sample of a human subject. In some cases, the method can comprise identifying sperm and/or prostate cells. Also described herein are methods of identifying cell types for forensic analysis and for the treatment of a disease. The methods disclosed herein may comprise determining the DNA methylation pattern of DNA extracted from a semen sample. Also disclosed herein are methods of treating a disease or condition of a subject.

Abstract: A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.

Abstract: Methods are provided of identifying a male fertility condition in a subject. Methods of performing a fertility treatment and an in vitro fertilization procedure are also provided. The methods may comprise determining the DNA methylation pattern of DNA extracted from a semen sample of a subject.

Abstract: Methods, systems, and computer programs are directed to the implementation of configurable hierarchical schedulers with multiple levels, where each level may use one of several types of queueing mechanisms. A configurable, hierarchical scheduler is designed to handle large scale processing of requests (e.g., transmitting outgoing messages). The hierarchical scheduler distributes the loads to different queues handling different types of messages (e.g., by user ID, by Internet Address (IP), by schedule). The different layers of the hierarchical scheduler are configurable to queue and schedule traffic based on many factors, such as IP address, handling reputation, available downstream bandwidth, fairness, concurrency rates to handle multiple constraints, scheduling per client, time of delivery constrains, rate limits per user, domain scheduling per user, concurrency throttling per outbound channel, and sharing global rate limits across service processors.

Abstract: Methods, systems, and computer programs are directed to the implementation of configurable hierarchical schedulers with multiple levels, where each level may use one of several types of queueing mechanisms. A configurable, hierarchical scheduler is designed to handle large scale processing of requests (e.g., transmitting outgoing messages). The hierarchical scheduler distributes the loads to different queues handling different types of messages (e.g., by user ID, by Internet Address (IP), by schedule). The different layers of the hierarchical scheduler are configurable to queue and schedule traffic based on many factors, such as IP address, handling reputation, available downstream bandwidth, fairness, concurrency rates to handle multiple constraints, scheduling per client, time of delivery constrains, rate limits per user, domain scheduling per user, concurrency throttling per outbound channel, and sharing global rate limits across service processors.

Abstract: A method of isolating sperm of a fluid sample comprises separating, in an initial separation operation, the fluid sample via a microfluidic separating system into a first debris fluid volume and a first sperm fluid volume, and then reflowing the first sperm fluid volume and a dilution fluid through the microfluidic separating system to recycle the first sperm fluid volume, and then separating, in a subsequent separation operation, the first sperm fluid volume into a second debris fluid volume and a second sperm fluid volume via the microfluidic separating system.

Abstract: Methods, systems, and computer programs are directed to the implementation of configurable hierarchical schedulers with multiple levels, where each level may use one of several types of queueing mechanisms. A configurable, hierarchical scheduler is designed to handle large scale processing of requests (e.g., transmitting outgoing messages). The hierarchical scheduler distributes the loads to different queues handling different types of messages (e.g., by user ID, by Internet Address (IP), by schedule). The different layers of the hierarchical scheduler are configurable to queue and schedule traffic based on many factors, such as IP address, handling reputation, available downstream bandwidth, fairness, concurrency rates to handle multiple constraints, scheduling per client, time of delivery constrains, rate limits per user, domain scheduling per user, concurrency throttling per outbound channel, and sharing global rate limits across service processors.

Abstract: A method for separating and enriching sperm from a tissue sample comprises: obtaining a microfluidic separating system having an inlet end and an outlet end, and a membrane filter (e.g., hollow fiber membrane filter) fluidly connected to the outlet end; separating the tissue sample via the microfluidic separating system into a debris fluid volume and a sperm fluid volume; and enriching the sperm fluid volume by removing excess media via the membrane filter. A two-stage tissue sample separation system comprising: a microchannel structure defining a separation fluid channel to form a separation stage; an inlet end of the microchannel structure; an outlet end of the microchannel structure; and a membrane filter fluidly connected to the outlet end for removal of at least a portion of excess media in the tissue sample.

Abstract: Methods are provided of identifying a male fertility condition in a subject. Methods of performing a fertility treatment and an in vitro fertilization procedure are also provided. The methods may comprise determining the DNA methylation pattern of DNA extracted from a semen sample of a subject.

Abstract: Provided is a high pressure polymorph of croconic acid. The high pressure polymorph of croconic acid has an unexpectedly high energy release and is suitable for use in detonable compositions. The high pressure polymorph of croconic acid is recoverable to ambient conditions and exhibits only a modest increase in density but a greatly improved energy release.

Abstract: Provided is a high pressure polymorph of croconic acid. The high pressure polymorph of croconic acid has an unexpectedly high energy release and is suitable for use in detonable compositions. The high pressure polymorph of croconic acid is recoverable to ambient conditions and exhibits only a modest increase in density but a greatly improved energy release.

Abstract: A network is set forth that comprises a plurality of multiple port switches, a plurality of endpoint devices connected to the ports of the plurality of switches, and an enumerating endpoint device that is connected to at least one of the multiple port switches. The enumerating endpoint device configures the routing through the multiple port switches based on whether the multiple port switch is on path with respect to a newly discovered processing element or off path with respect to the newly discovered processing element. Each off path switch may be configured so that communication packets destined for the new processing element are routed through the port of the off path switch that the off path switch uses for communications with the enumerating endpoint device.

Abstract: A network is set forth that comprises a plurality of multiple port switches, a plurality of endpoint devices connected to the ports of the plurality of switches, and an enumerating endpoint device that is connected to at least one of the multiple port switches. The enumerating endpoint device configures the routing through the multiple port switches based on whether the multiple port switch is on path with respect to a newly discovered processing element or off path with respect to the newly discovered processing element. Each off path switch may be configured so that communication packets destined for the new processing element are routed through the port of the off path switch that the off path switch uses for communications with the enumerating endpoint device.

Abstract: The present invention relates, inter alia, to the crystal structures of a phosphodiesterase 5 (PDE5) and PDE5/PDE5 ligand complex and their uses in identifying PDE5 ligands, including PDE5 inhibitor compounds. The present invention also relates to methods of identifying such PDE5 inhibitor compounds and their medical use. Also contemplated by the present invention are crystals of PDE5/PDE5 inhibitor complexes.

Abstract: The present invention relates to the soakable crystals of a phosphodiesterase 5 (PDE5) and their uses in identifying PDE5 ligands, including PDE5 ligands and inhibitor compounds. The present invention also relates to methods of identifying such PDE5 inhibitor compounds and their medical use. The present invention additionally relates to crystals of PDE5 into which ligands may be soaked and crystals of PDE5 comprising PDE5 ligands that have been soaked into the crystal.