Abstract: Described herein are novel biological converter switches that utilize modular components, such as genetic toggle switches and single invertase memory modules (SIMMs), for converting analog inputs to digital outputs, and digital inputs to analog outputs, in cells and cellular systems. Flexibility in these biological converter switches is provided by combining individual modular components, i.e., SIMMs and genetic toggle switches, together. These biological converter switches can be combined in a variety of network topologies to create circuits that act, for example, as switchboards, and regulate the production of an output product(s) based on the combination and nature of input signals received.

Abstract: Provided herein are methods and compositions for dynamically controlling and targeting multiple arms of the immune system. Some aspects provide mesenchymal stem cells (MSCs) engineered to produce multiple effector molecules. In some instances, each effector molecule modulates a different cell type of the immune system or different functions of a cell. Also provided herein are methods of using the MSCs to treat or alleviate symptoms of inflammatory bowel disease (IBD), for example.

Abstract: Disclosed herein are novel 5? UTR sequences that can be used to control gene expression in various contexts. Also disclosed herein are methods of engineering 5? UTR sequences and methods and kits for screening 5? UTR sequences for a property of interest.

Abstract: Provided herein are compositions, systems, and methods for information (e.g., artificial or digital information) recording and storage in nucleic acids (e.g., DNA). Information can be recorded and stored on pre-synthesized storage medium.

Abstract: Provided herein are compositions, systems, and methods for continuous and accumulative modification of a target site.

Abstract: Provided herein are recombinase-based frameworks for building state machines in vitro and in vivo by using chemically controlled DNA excision and inversion operations to encode state in DNA sequence.

Abstract: Disclosed are methods of antimicrobial treatment using ionic liquids (ILs), IL complexes, polymers comprising ILs, and polymers comprising neutral ethylene diamine compounds. Also disclosed are novel IL complexes, polymers comprising ILs, and polymers comprising neutral ethylene diamine compounds.

Abstract: Compositions, methods, and kits are provided which involve novel fusion protein-based adhesives for use in medical and marine applications. In some aspects, the novel fusion proteins comprise an adhesive domain (e.g., derived from an adhesive protein of a marine organism) and an amyloid domain (e.g., derived from a bacterial amyloid protein). Also provided are copolymers and fibers of the fusion proteins.

Abstract: Disclosed herein are novel devices comprising small, ultra-low power microelectronic components. In some instances, the microelectronic components is combined with a biosensor component that enables in situ detection of biomolecules. Also disclosed herein are methods of detecting signal analytes and methods of monitoring the health of a patient using these novel devices.

Abstract: Disclosed herein are methods of designing peptides having at least one property of interest, such as ?-helical propensity, higher net charge, hydrophobicity, and/or hydrophobic moment. Also disclosed herein are novel artificially evolved peptides (e.g., antimicrobial peptides), which may be designed according to the methods described herein, and methods of use thereof.

Abstract: Aspects of the invention relate to the engineering of biological nanostructures and materials.

Abstract: Provided herein, in some embodiments, are hydrogel-elastomer and hydrogel-alginate devices, compositions and associated methods to encapsulate living cells.

Abstract: Provided herein, in some embodiments, are methods, compositions, systems and kits that enable spatiotemporal regulation of nucleic acid expression in engineered cells.

Abstract: Described herein are methods and compositions for the rapid production of therapeutic molecules using an inducible cell culture system.

Abstract: Provided herein, in some embodiments, are methods, compositions, systems and kits that enable removal of heterologous nucleic acid from engineered cells.

Abstract: Various aspects and embodiments of the invention are directed to high-throughput phage-engineering methods and recombinant bacteriophages with tunable host ranges for controlling phage specificity.

Abstract: Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.

Abstract: Disclosed herein are novel methodologies for cloning prophage genome sequences that are identified from target organisms or DNA sequencing data and that contain mutations that decrease the function of prophage repressor proteins and for producing lytic phage particles with decreased prophage repressor protein function.

Abstract: Compositions and methods using shufflon recombinases are presented for use in generating genetic diversity in molecules of interest.

Abstract: Aspects of the present disclosure provide a platform that triggers potent and effective immunotherapy against tumors from within tumors themselves, thus overcoming limitations of existing cancer immunotherapies and tumor-detecting gene circuits.