Abstract: This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules.

Abstract: This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules.

Abstract: This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules.

Abstract: A miniaturized Surface Plasmon Resonance (SPR) imaging system (20) is provided, which includes a light source (21), a sensor substrate (26c) arranged to receive light at an incident angle from the light source, and a detector (31) for detecting an image from the sensor substrate. The system further includes a folded light path structure (23) arranged between the light source and the detector. The folded light path structure includes the sensor substrate (26c), and is configured so as to receive the light from the light source, to redirect the received light to be incident on the sensor substrate (26c) at the incident angle (first redirection), and to further redirect the light reflected from the sensor substrate (26c) toward the detector (second redirection). The folded light path structure allows for a minimal instrumentation footprint, which in turn allows for the construction of a miniaturized, hand-portable SPR imaging system.

Abstract: This specification discloses various improvements in the field of SPR sensing systems. One improvement relates to a portable SPR sensing system, e.g., a system contained within a suitcase that can be hand-carried to a monitoring site. Another improvement relates to a portable, cartridge-based SPR sensing system. In this system, selected portions of the system's electrical and fluidics systems are allocated between a base unit and a removable/disposable cartridge. Other improvements relate to methods or protocols for operating an SPR sensing system. Such methods provide for the elimination of false positives and increased sensitivity, e.g., by using secondary antibodies with specificity for different target epitopes and by sensor element redundancy. In addition, protocols are provided for the detection of small molecules.

Abstract: A surface plasmon resonance imaging system (40) and method is provided. The system (40) includes a light source (42) comprising a light-emitting diode (LED) array that is positioned at the focal point of a collimating lens (44). The light source (42) and collimating lens (44) are used to illuminate the substrate surface (50) at a range of angles dependent upon which one or more LEDs are lit. The substrate surface (50) receives light from the collimated lens (44) at a selected incident angle, which can be varied by selective illumination of one or more of the LEDs in the LED array. The system (40) further includes a detector (60) that is positioned such that it is capable of detecting an image reflected from the substrate surface (50).

Abstract: SPR sensors and multisensors having capillary geometry. Sensors have a capillary substrate in which at least a portion of the inside surface of the capillary is provided with an SPR-sensing area. Samples for analysis are introduced into the capillary cavity. SPR is measured by radially illuminated the capillary SPR-sensing area with light having a TM-polarized component. Light reflected from the SPR-sensing area exiting radially from the capillary is detected at selected angles to obtain reflectivity as a function of incidence angle to determine RI of the sample in the vicinity of the SPR-sensing area. The capillary geometry is readily adaptable to simultaneous measurement of several optical properties of a given sample in addition to SPR by radial and/or axial illumination of the sample. Multisensors with capillary geometry which simultaneously measure SPR and bulk RI are provided.