Patents by Inventor Timothy Piazza

Timothy Piazza has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Reporting construct with synaptobrevin based moiety
    Patent number: 12287323
    Abstract: Compositions and methods for improved cell-based methods of characterizing botulinum neurotoxins are provided. Cells utilized in these methods include a reporting construct that is cleaved following uptake and processing of botulinum neurotoxin by the cell, resulting in proteolysis of the portion of the reporting construct that is released following cleavage. The released portion includes a fluorophore and amino acid substitutions or sequences that enhance the rate of proteolysis. A pair of reporting constructs can be utilized in which one member of the pair is modified to resist cleavage by the botulinum neurotoxin while co-localizing with the remaining member of the pair.
    Type: Grant
    Filed: January 25, 2022
    Date of Patent: April 29, 2025
    Assignee: BIOMADISON, INC.
    Inventors: Timothy Piazza, Francis Mark Dunning, Ward C Tucker
  • Assay with synaptobrevin based moiety
    Patent number: 12019066
    Abstract: Compositions and methods for improved cell-based methods of characterizing botulinum neurotoxins are provided. Cells utilized in these methods include a reporting construct that is cleaved following uptake and processing of botulinum neurotoxin by the cell, resulting in proteolysis of the portion of the reporting construct that is released following cleavage. The released portion includes a fluorophore and amino acid substitutions or sequences that enhance the rate of proteolysis. A pair of reporting constructs can be utilized in which one member of the pair is modified to resist cleavage by the botulinum neurotoxin while co-localizing with the remaining member of the pair.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: June 25, 2024
    Assignee: BioMadison, Inc.
    Inventors: Timothy Piazza, Francis Mark Dunning, Ward C Tucker
  • Assay with Synaptobrevin Based Moiety
    Publication number: 20220196634
    Abstract: Compositions and methods for improved cell-based methods of characterizing botulinum neurotoxins are provided. Cells utilized in these methods include a reporting construct that is cleaved following uptake and processing of botulinum neurotoxin by the cell, resulting in proteolysis of the portion of the reporting construct that is released following cleavage. The released portion includes a fluorophore and amino acid substitutions or sequences that enhance the rate of proteolysis. A pair of reporting constructs can be utilized in which one member of the pair is modified to resist cleavage by the botulinum neurotoxin while co-localizing with the remaining member of the pair.
    Type: Application
    Filed: January 25, 2022
    Publication date: June 23, 2022
    Inventors: Timothy Piazza, Francis Mark Dunning, Ward C Tucker
  • ASSAY WITH SYNAPTOBREVIN BASED MOIETY
    Publication number: 20180074044
    Abstract: Compositions and methods for improved cell-based methods of characterizing botulinum neurotoxins are provided. Cells utilized in these methods include a reporting construct that is cleaved following uptake and processing of botulinum neurotoxin by the cell, resulting in proteolysis of the portion of the reporting construct that is released following cleavage. The released portion includes a fluorophore and amino acid substitutions or sequences that enhance the rate of proteolysis. A pair of reporting constructs can be utilized in which one member of the pair is modified to resist cleavage by the botulinum neurotoxin while co-localizing with the remaining member of the pair.
    Type: Application
    Filed: May 16, 2017
    Publication date: March 15, 2018
    Inventors: Timothy Piazza, Francis Mark Dunning, Ward C. Tucker