Abstract: Disclosed herein are methods and systems for predicting a subject's response to immunotherapy to treat a cancer, including receiving sequencing data of the subject; determining, using the sequencing data, a plurality of somatic features for the subject and a plurality of germline features for the subject; generating, using the plurality of somatic features for the subject and the plurality of germline features for the subject, an immune checkpoint blockade (ICB) response score for the subject to represent a likelihood of response to an immunotherapy for the subject; and comparing the ICB response score for the subject to an ICB response threshold value.

Abstract: A device, method and system for disease detection relating to the selective capture of an antigen in an analyte by a linked capture antibody, where the linked capture antibody is expressed on a nanobiosensing chip as a plurality of non-randomly oriented binding sites, within a functionalized surface, that are upwardly oriented. The device, method and system enable selective capture of an antigen in an analyte with a selectivity and sensitivity that is greater than that attainable without the plurality of non-randomly oriented binding sites that are upwardly oriented and active. The device, method and system enable selective detection of an antigen in an analyte.