Abstract: The invention relates to one or more mutations configured to stabilize the prefusion “up” protomer trimeric Spike protein from SARS-CoV-2. The inventive technology further relates to systems, methods, and compositions to display one or more proteins on the surface of a yeast cell. Specifically, in one embodiment the invention relates to systems, methods, and compositions to display one or more Spike protein from SARS-CoV-2 on the surface of a yeast cell, and more preferably a Spike protein from SARS-CoV-2 stabilized in its prefusion conformation on the surface of a yeast cell.

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

Abstract: The invention relates to one or more mutations configured to stabilize the prefusion “up” protomer trimeric Spike protein from SARS-CoV-2. The inventive technology further relates to systems, methods, and compositions to display one or more proteins on the surface of a yeast cell. Specifically, in one embodiment the invention relates to systems, methods, and compositions to display one or more Spike protein from SARS-CoV-2 on the surface of a yeast cell, and more preferably a Spike protein from SARS-CoV-2 stabilized in its prefusion conformation on the surface of a yeast cell.

Abstract: Deep mutational scanning is a foundational tool for addressing functional consequences of large numbers of mutants, yet a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Provided herein are nicking saturation mutagenesis, a single-day, single-pot saturation mutagenesis method using routinely prepped plasmid dsDNA as input substrate. Reproducibility and convenience of the method are demonstrated through validation by an external research laboratory.

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

Abstract: Deep mutational scanning is a foundational tool for addressing functional consequences of large numbers of mutants, yet a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Provided herein are nicking saturation mutagenesis, a single-day, single-pot saturation mutagenesis method using routinely prepped plasmid dsDNA as input substrate. Reproducibility and convenience of the method are demonstrated through validation by an external research laboratory.

Abstract: The disclosure relates to methods for obtaining nucleic acid sequence information by constructing a nucleic acid library and reconstructing longer nucleic acid sequences by assembling a series of shorter nucleic acid sequences.

Abstract: The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as ?-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.

Abstract: The invention provides methods and compositions for increasing tolerance of microorganisms to toxic agents, such as solvents; and for increasing production of solvents from solvent-generating microorganisms. The methods comprise engineering a microorganism of interest to express a heterologous heat-shock protein/chaperone, e.g., Group II chaperonin or a prefoldin such as ?-prefoldin, where the heterologous protein is from an extremophile, such as an archaean.