Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: System and methods for geosteering inversion are provided. Downhole tool responses are predicted for different points along a planned path of a wellbore during a downhole operation, based on each of a plurality of inversion models. Measurements of the downhole tool's actual responses are obtained as the wellbore is drilled over the different points during a current stage of the operation. The inversion models are then clustered using a weighted clustering approached in which a weighted score system is formulated to apply a standard deviation, number of models, and/or misfit of each cluster as key parameters, and used to evaluate the clusters accordingly. The corresponding scores are computed and used to select clusters. The planned wellbore path is adjusted for the subsequent stage(s) of the downhole operation.

Abstract: The provided is an adjustable single-channel time-domain sampling filter, a spectrometer, and a detection method. The filter includes N stages of cascaded asymmetric interference units, where each asymmetric interference unit includes: a first waveguide splitting element, arranged at the input end, for splitting input light into two output paths; two interference arms, which respectively receive the light output from the two paths of the first waveguide splitting element and have different arm lengths; and a second waveguide splitting element, for combining the output light of the two interference arms to generate optical interference. Among the N cascaded asymmetric interference units, at least three are provided with phase modulators on their interference arms. When in use, the adjustable single-channel time-domain sampling filter tunes the phase modulators located in different cascaded asymmetric interference units to form optical channels with different spectral responses in the time sequence.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: Provided is a recombinant adeno-associated virus (rAAV) vector comprising a nucleic acid molecule encoding a chimeric antigen receptor (CAR) which specifically binds to a central nervous system (CNS) tumor cell, preferably a solid CNS tumor cell. Further provided is a modified cell comprising a chimeric antigen receptor (CAR) which is obtained by transducing the cell with the rAAV vector, and a method for treating a CNS tumor using the rAAV vector or modified cell of the present disclosure.

Abstract: A computer-implemented method to perform joint inversion of formation data includes performing a first inversion of a formation surrounding a wellbore at a first frequency/spacing configuration, and performing a second inversion of the formation at a second frequency/spacing configuration that is different from the first configuration. The method also includes assigning a first fluctuating weight to the first inversion, and assigning a second fluctuating weight to the second inversion. The method further includes merging the first and second inversion based on a combination of the first and second fluctuating weights.

Abstract: This application provides an air fryer with excellent cooking performance, including a body, a cooking cavity located inside the body, and a frying system, the cooking cavity is connected with the frying system, the body has multiple seasoning boxes on the outside, and the body has multiple feeding mechanisms corresponding to the seasoning boxes inside, one end of the multiple feeding mechanisms is connected with the corresponding seasoning box, and the other end is connected with the cooking cavity.

Abstract: Features described herein generally relate to an electronic device and a method for activity detection. Particularly, an electronic device can be detected as being in a docked mode and/or a tablet mode. In the docked mode, activity can be detected based on a first detector. In the tablet mode, activity can be detected based on a second detector. The activity can be classified as corresponding to an activity type and a display screen of the electronic device can be updated based on the activity type.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the 10 transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-type Aspergillus niger glucose oxidase or a mutant Aspergillus niger glucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: System and methods for geosteering inversion are provided. Downhole tool responses are predicted for different points along a planned path of a wellbore during a downhole operation, based on each of a plurality of inversion models. Measurements of the downhole tool's actual responses are obtained as the wellbore is drilled over the different points during a current stage of the operation. The inversion models are clustered based on a comparison between the actual and predicted tool responses and a randomly selected centroid for each cluster. The inversion models are re-clustered using an average inversion model determined for each cluster as the centroid for that cluster. At least one of the re-m clustered inversion models is used to perform inversion for one or more subsequent stages of the downhole operation along the planned wellbore path. The planned wellbore path is adjusted for the subsequent stage(s) of the downhole operation.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

Abstract: A chimeric lysozyme variant has an improved heat resistance and is encoded by a polynucleotide. A novel chimeric lysozyme sequence is constructed, and high-efficiency expression in a host cell is achieved. The thermal stability of the chimeric lysozyme under the condition of 60° C. is nearly doubled compared to a parental sequence. The chimeric lysozyme variant can be used to prepare product that is antibiotic, for example, animal feed additive containing the chimeric lysozyme variant and an animal feed containing the animal feed additive.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (l) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: The disclosure provides a method for breeding Carassius auratus strains without intermuscular bones, which comprises designing knockout target sites for the two copies of the bmp6, bmp6a and bmp6b, in the Carassius auratus genome, and then obtaining F2 generation individuals from a homozygous line with bmp6a and bmp6b double gene mutation through two rounds of gene knockout and screening, and then propagating by using the F2 generation individuals from the homozygous line with bmp6a and bmp6b double gene mutation to form a new Carassius auratus strain with intermuscular bone-deficient. Strains of Carassius auratus with less than 20 intermuscular bones and without intermuscular bones are obtained by the method of the present disclosure.

Abstract: Provided is a method for large-scale production of lentivirus by using GMP-level serum-free suspension cells. Said method comprises the following steps: (a) providing a seed solution of packaged cells; (b) inoculating the seed solution in a first culture solution; (c) carrying out subculture of the packaged cells; (d) starting a liquid change operation when a liquid change trigger condition is met; (e) repeating steps (c) and (d) 1, 2 or 3 times; (f) starting a transfection operation when a transfection trigger condition is met; (g) optionally performing liquid change after transfection; (h) cultivating the transfected packaged cells; (i) starting harvesting and liquid change operations when a liquid change trigger condition is met; (j) repeating steps (h) and (i) 1, 2 or 3 times; (k) combining each of the recovered liquids; and (1) performing a purifying treatment. The culture solution used in each step is a serum-free cell culture solution.

Abstract: The present invention relates to a Bragg grating chip, which comprises a monocrystalline silicon substrate, a silicon dioxide layer arranged on the monocrystalline silicon substrate, a Bragg grating arranged on the silicon dioxide layer and a negative thermal-optical coefficient material arranged on the Bragg grating, so that the sensitivity of the Bragg grating based on lithium niobate crystals to temperature is eliminated, the drift amount of the reflection spectrum center wavelength of the Bragg grating chip in the environment temperature change of 1 k is basically zero, and the insensitivity of the spectral response of photoelectric devices such as optical filter, laser and the like formed by the lithium niobate Bragg grating to the temperature change can be realized.