Abstract: Probiotics for the purine-degrading capability under the condition of a rich-nutrient medium are screened, and a culture environment relatively simulating the human intestinal tract is obtained, so that Lactobacillus reuteri strains KLR-1, KLR-3, KLR-4 and KLR-13 are obtained. The probiotic strain or the composition containing the strain has a significant serum uric acid-reducing effect in an animal model. The composition has a probiotic-containing composition and prebiotics. The probiotic-containing composition is one or more of Lactobacillus reuteri, Lactobacillus ferrnenturn, Bacillus coagulans, Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus plantarum. The prepared compound probiotic powder can significantly reduce the serum uric acid of gout patients and has the effect of dissolving urate crystals.