Abstract: In one embodiment, the invention provides a method of diagnosing sepsis or a virus-related infection (often a viral hemorrhagic fever infection) in a subject by detecting and measuring the level of a set of sepsis and virus infection-associated-GTPase biomarkers in a sample obtained from the subject using multiplexed flow cytometry. Related kits are also provided. In a preferred embodiment, the invention provides point of care diagnostic methods for determining an early stage sepsis or the severity of a virus infection, especially in a hospital or other setting.

Abstract: The present invention is directed to the discovery that RapI-GTP, Racl-GTP and Flt3-L can be used as Biomarkers for the early detection of sepsis in patients suspected of a bacterial infection which presents as sepsis or is likely to produce sepsis. In particular, the present invention is directed to methods, assays and kits which may be used to distinguish sepsis (infection) from systematic inflammatory response caused by sterile inflammation in trauma patients. The method may he used to diagnose bacteria infection and/or sepsis and monitor therapy of a patient to allow modification of treatment and/or cessation of treatment.

Abstract: In one embodiment, the invention provides a method of diagnosing sepsis or a virus-related infection (often a viral hemorrhagic fever infection) in a subject by detecting and measuring the level of a set of sepsis and virus infection-associated-GTPase biomarkers in a sample obtained from the subject using multiplexed flow cytometry. Related kits are also provided. In a preferred embodiment, the invention provides point of care diagnostic methods for determining an early stage sepsis or the severity of a virus infection, especially in a hospital or other setting.

Abstract: In one embodiment, the invention provides a method of diagnosing sepsis or a virus-related infection (often a viral hemorrhagic fever infection) in a subject by detecting and measuring the level of a set of sepsis and virus infection-associated-GTPase biomarkers in a sample obtained from the subject using multiplexed flow cytometry. Related kits are also provided. In a preferred embodiment, the invention provides point of care diagnostic methods for determining an early stage sepsis or the severity of a virus infection, especially in a hospital or other setting.

Abstract: In one embodiment, the invention provides a method of diagnosing sepsis or a virus-related infection (often a viral hemorrhagic fever infection) in a subject by detecting and measuring the level of a set of sepsis and virus infection-associated-GTPase biomarkers in a sample obtained from the subject using multiplexed flow cytometry. Related kits are also provided. In a preferred embodiment, the invention provides point of care diagnostic methods for determining an early stage sepsis or the severity of a virus infection, especially in a hospital or other setting.

Abstract: The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.

Abstract: The present invention provides functionalized mesoporous microspheres comprising a microsphere including surface mesopores; a lipid bilayer covering at least a portion of the microsphere; and enclosed mesoporous spaces comprising the surface mesopores and respective portions of the lipid bilayer.

Abstract: The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.

Abstract: A mixing apparatus is provided comprising: first driving means for driving a plurality of reagent samples from a plurality of respective source wells into a first fluid flow stream; second driving means for introducing a separation gas between each of the plurality of reagent samples in the first fluid flow stream; means for driving a second fluid flow stream comprising a plurality of particles; a junction device comprising: a first inlet port for receiving the first fluid flow stream; a second inlet port for receiving the second fluid flow stream; a reaction zone for forcing mixing between the first fluid flow stream and the second fluid flow stream to thereby form a reaction product stream; and an outlet port for allowing the reaction product stream to exit the junction device; a reaction zone where the plurality of reagent samples and the plurality of particles mix to form a plurality of reaction products, the reaction zone communicating with the outlet port; reaction product driving means for driving the

Abstract: The invention provides a sensing device comprising: a vessel; a plurality of sensor beads located within the vessel to form interstitial spaces therethrough; and a plurality of biomolecules bound to at least at portion of the plurality of beads, each of the biomolecules having a fluorescent tag.