Abstract: The present invention relates to a biodegradable detergent composition, which can contain viable cells or enzymes and acts as a replacement for the chemical detergents. The biodegradable detergent composition, comprising of: bio surfactant; polyols; biopolymer; organic acid; small chain peptide; essential oil; and stabilizers. The biodegradable detergent composition has a pH value of less than about 3.60, Titratable Acidity % between about 9.0-12.0, HLB value between about 14 to about 16.5, E24 value between about 73 and about 79. The biodegradable detergent composition has MALDI-TOF MS spectra in the m/z range from about 513.1 to about 2847.7 with peak mass value range from about 1375.8 m/z to about 2009.2 m/z. Further, the present invention relates to a method of manufacturing the biodegradable detergent composition.