Abstract: The present invention generally relates to the provision of means and methods for preparing protein compositions. The invention provides a method comprising the steps of (i) contacting a starting composition comprising a protein with a hydrolase inhibitor, wherein the hydrolase inhibitor is immobilized on a solid carrier; (ii) recovering a composition comprising the protein. Furthermore, the invention relates to a method for preparing a protein formulation comprising the steps of (i) contacting a starting composition comprising a protein with a hydrolase inhibitor, wherein the hydrolase inhibitor is immobilized on a solid carrier; (ii) recovering a composition comprising the protein, wherein the method for preparing the protein formulation further comprises adding a surfactant, preferably a fatty acid ester, to the composition comprising the protein.

Abstract: Herein is reported a method for determining the presence of hydrolase activity in a sample by incubating the sample in a buffered solution, which comprises an artificial hydrolase substrate that comprises an ester bond covalently linking an alcohol residue, which is conjugated to a label for removal, and a carboxylic acid residue, which is conjugated to a capture and detection label, as well as bovine serum albumin, and thereafter determining or quantifying, respectively, the released carboxylic acid in the free alcohol depleted incubation mixture.

Abstract: A guide device for a diffusion vacuum pump having a plurality of annular guide plates. The guide plates being arranged radially, wherein at least one guide plate has a first section starting from a connection point and a second section starting from the connection point. Therein, the first section extends axially in a first direction, the second section extending axially in the opposite second direction. Therein the first section is designed to taper radially and/or the second section is designed to taper radially.

Abstract: An ophthalmic lens for eyeglasses. The lens has an eye side, a surroundings side, a waveguide, a first compensation layer, a second compensation layer, a first outer layer, and a second outer layer. The waveguide is formed to conduct and couple out projection light in the direction of the eye side, in order to display a virtual object. The first compensation layer has a first refractive power, the second compensation layer has a second refractive power, the first outer layer has a third refractive power, and the second outer layer has a fourth refractive power.

Abstract: An apparatus and method for recording a holographic optical element. The apparatus includes a first recording unit configured to provide a first wave front for recording the holographic optical element, a second recording unit configured to provide a second wave front for recording the holographic optical element, and (i) a deformable phase plate configured to perform wave front modulation of the first wave front when the holographic optical element is recorded, or (ii) a plurality of deformable phase plates, at least one deformable phase plate (of the plurality of deformable phase plates can be configured to perform wave front modulation of the first wave front when the holographic optical element is recorded.

Abstract: A method for calibrating a projection device for a head-mounted display includes scanning a light beam emitted by a light source over a scanning angle range by means of a reflection element such that the light beam deflected by the reflection element passes over a head-mounted display surface region of a deflection element arranged on a lens of the head-mounted display. The surface region has at least two adjustment markings arranged on the head-mounted display, each adjustment marking arranged at a specified position relative to the surface of the deflection element arranged on a lens of the head-mounted display. The method further includes determining in which scan setting of the reflection element the at least two adjustment markings are struck by the light beam.

Abstract: A method and a laser assemblage are described for material processing, such that in a laser assemblage, a laser beam is focused onto a processing/imaging plane and the laser beam can be adapted in terms of its intensity distribution by way of at least one beam shaper. Provision is made in this context that in order to avoid uniformity defects in the processing/imaging plane, the laser beam is split by way of at least one beam splitter into at least two partial or individual beams, and the partial or individual beams are differently influenced, or each partial or individual beam is constituted from a laser source having a different wavelength, in such a way that after they are combined and focused onto the processing/imaging plane they form an output beam having an intensity profile, adjacent intensity maxima of the intensity profile differing in terms of their light properties.

Abstract: The disclosure relates to a device for providing a multi-colored light beam for a projector. The device comprises a first light source, a second light source, a waveguide element, a beam forming device and a structure element. The waveguide element forms a first waveguide for guiding light from the first light source, a second waveguide for guiding light from the second light source and a coupling out region for coupling light out of the first waveguide and the second waveguide. The beam forming device is designed to form the multi-colored light beam using the light coupled out of the coupling out region. The first light source, the second light source, the waveguide element and the beam forming device are arranged on the structure element.

Abstract: The present disclosure provides purification platforms comprising a depth filter step and/or a hydrophobic interaction chromatography (HIC) step. Also disclosed herein are methods of using the purification platforms described herein and compositions obtained therefrom, such as pharmaceutical compositions.

Abstract: An optical switch includes a bus waveguide and an optical antenna supported by a substrate, a first and second coupling waveguide, a first and second actuation electrode, and a first and second reaction electrode. The first coupling waveguide is disposed parallel with the substrate and aligned with the bus waveguide. The first reaction electrode is coupled with, and adjacent to, the first coupling waveguide. The second coupling waveguide is optically connected with the first coupling waveguide and suspended over and configured to optically couple with the optical antenna. The second reaction electrode is coupled with, and adjacent to, the second coupling waveguide. The first and second actuation electrodes are supported by the substrate and configured to control the position of the first and second coupling waveguide, respectively, relative to the bus waveguide and optical antenna, via the first and second reaction electrodes.

Abstract: An optical switch includes a bus waveguide and an optical antenna supported by a substrate, a first and second coupling waveguide, a first and second actuation electrode, and a first and second reaction electrode. The first coupling waveguide is disposed parallel with the substrate and aligned with the bus waveguide. The first reaction electrode is coupled with, and adjacent to, the first coupling waveguide. The second coupling waveguide is optically connected with the first coupling waveguide and suspended over and configured to optically couple with the optical antenna. The second reaction electrode is coupled with, and adjacent to, the second coupling waveguide. The first and second actuation electrodes are supported by the substrate and configured to control the position of the first and second coupling waveguide, respectively, relative to the bus waveguide and optical antenna, via the first and second reaction electrodes.

Abstract: The disclosure relates to a device for providing a multi-colored light beam for a projector. The device comprises a first light source, a second light source, a waveguide element, a beam forming device and a structure element. The waveguide element forms a first waveguide for guiding light from the first light source, a second waveguide for guiding light from the second light source and a coupling out region for coupling light out of the first waveguide and the second waveguide. The beam forming device is designed to form the multi-colored light beam using the light coupled out of the coupling out region. The first light source, the second light source, the waveguide element and the beam forming device are arranged on the structure element.

Abstract: A method for producing a holographic optical element (HOE) that is provided for projection in a projection system. A hologram is recorded by the fact that a first Gaussian beam and a second Gaussian beam are caused to interfere on a holographic film for at least two different configurations. The first Gaussian beam is a reference beam that, for the at least two different configurations, is identical to a reconstruction beam with which the HOE is reconstructed. The second Gaussian beam is furthermore an object beam that, upon reconstruction of the HOE utilizing the reconstruction beam, is identical to a projection beam that is used in the projection system for projection. For the at least two different configurations, at least one beam property that depends respectively on predefined projection properties of the projection system is predefined for the second Gaussian beam.

Abstract: A diffractive optical element includes at least two diffractive structures situated next to one another, having differing functionalities, and being configured to, responsive to being irradiated independently of one another with incoherent laser light from beams of their respectively coupled laser sources, generate respective diffraction patterns that do not interfere with one another and that combine as an overall diffraction pattern in a far field. A method is provided for manufacturing such a diffractive optical element.

Abstract: An optical assembly, for receiving light waves, includes a receiving optical system for focusing at least one incoming light wave onto a surface of a detector for detecting the at least one light wave, at least one diffractive optical element with a planar extension being situated between the receiving optical system and the detector, and the at least one diffractive optical element including a surface with a surface structure with at least one optical function. Furthermore, a LIDAR device includes an optical assembly of this type.

Abstract: A method for calibrating a projection device for a head-mounted display includes scanning a light beam emitted by a light source over a scanning angle range by means of a reflection element such that the light beam deflected by the reflection element passes over a head-mounted display surface region of a deflection element arranged on a lens of the head-mounted display. The surface region has at least two adjustment markings arranged on the head-mounted display, each adjustment marking arranged at a specified position relative to the surface of the deflection element arranged on a lens of the head-mounted display. The method further includes determining in which scan setting of the reflection element the at least two adjustment markings are struck by the light beam.

Abstract: A projection device for a head-mounted display includes at least one light source for emitting at least one light beam, at least one collimation element for collimating the at least one light beam emitted by the light source, at least one deflecting element which is arranged or can be arranged on a lens of the head-mounted display for projecting an image onto a retina of a user of the head-mounted display by deflecting and/or focusing the at least one light beam onto an eye lens of the user, and at least one reflection element for reflecting the collimated light beam onto the deflection element. The projection device further includes at least one optical correction element which has a rotationally non-symmetrical optical element for improving the symmetry of the light beam and/or for reducing the spot size of the light beam.

Abstract: A method and a laser assemblage are described for material processing, such that in a laser assemblage, a laser beam is focused onto a processing/imaging plane and the laser beam can be adapted in terms of its intensity distribution by way of at least one beam shaper. Provision is made in this context that in order to avoid uniformity defects in the processing/imaging plane, the laser beam is split by way of at least one beam splitter into at least two partial or individual beams, and the partial or individual beams are differently influenced, or each partial or individual beam is constituted from a laser source having a different wavelength, in such a way that after they are combined and focused onto the processing/imaging plane they form an output beam having an intensity profile, adjacent intensity maxima of the intensity profile differing in terms of their light properties.

Abstract: An optical assembly, for receiving light waves, includes a receiving optical system for focusing at least one incoming light wave onto a surface of a detector for detecting the at least one light wave, at least one diffractive optical element with a planar extension being situated between the receiving optical system and the detector, and the at least one diffractive optical element including a surface with a surface structure with at least one optical function. Furthermore, a LIDAR device includes an optical assembly of this type.

Abstract: A diffractive optical element includes at least two diffractive structures situated next to one another, having differing functionalities, and being configured to, responsive to being irradiated independently of one another with incoherent laser light from beams of their respectively coupled laser sources, generate respective diffraction patterns that do not interfere with one another and that combine as an overall diffraction pattern in a far field. A method is provided for manufacturing such a diffractive optical element.