Abstract: The present invention provides a method for predicting a remaining lifetime of an electrical component of an electrical circuit, the electrical circuit being part of a building management device. The method comprises: estimating (S1) two or more estimated temperatures of the electrical component by using a trained machine learning model of the electrical component that is trained based on training data. Further, the method comprises: generating (S2) a temporal course of temperature of the electrical component based on the two or more estimated temperatures; and computing (S3), based on the temporal course of temperature of the electrical component, an indicator for the remaining lifetime of the electrical component. The present invention also provides a method for predicting a remaining lifetime of an electrical circuit being part of a building management device.

Abstract: The invention relates to a rod section of a ground drilling rod, wherein the rod section is configured at the end to form a plug connection and has (a) a male connector or (b) a female connector at at least one end, wherein the male connector has at least one circular portion and at least one more straight-line-like portion in cross section on the outside, or the female connector has at least one circular portion and at least one more straight-line-like portion in cross section on the inside.

Abstract: The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: The present invention relates to the field of industrial fermentation.

Abstract: The present invention relates to the field of industrial fermentation.

Abstract: The present invention relates to the field of industrial fermentation. In particular, it relates to method for producing a protein of interest from a Bacillus host cell comprising the steps of inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, cultivating the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest for a cultivation phase, wherein the cultivation of the Bacillus host cell during said cultivation phase comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source, and separating portions from said Bacillus host cell culture during the cultivation phase at different time points and obtaining the protein of interest from said portions.

Abstract: The present invention relates to a Bacillus host cell belonging to the species Bacillus lichemformis or Bacillus pumilus in which the chromosomal alr gene has been inactivated. Said bacterial host cell comprises a plasmid comprising at least one autonomous replication sequence, a first polynucleotide encoding at least one polypeptide of interest, wherein said first polynucleotide is operably linked to a promoter, and a second polynucleotide encoding an alanine racemase which is not native to the host cell, wherein said second polynucleotide is operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: The invention relates to a process for culturing microbial cells producing a product of interest comprising fed-batch pre-culture.

Abstract: A rod section of a ground drilling rod configured at its end to form at least one plug connection and having at one end (a) a connecting plug with an outer contour; or (b) a connecting socket with an inner contour, the outer contour or the inner contour being essentially sinusoidal in cross section.

Abstract: A method for certificate chain validation is disclosed for memory-efficient execution inside a secure execution environment of a hardware security module (HSM), despite very limited memory being inside the HSM. This is achieved by allocating enough HSM memory for at least the root certificate, a signer certificate, and a certificate to be validated, and replacing the signer certificate as the certificate chain is traversed. Revalidation of data is implemented by computing secure tags that enable the HSM to quickly recognize previously validated data. Chain validation is only considered provisional until checked against a CRL, which can be provided to the HSM in a streaming fashion, and each received serial number in the CRL is checked against the list of serial numbers representing the provisionally verified chain.

Abstract: The invention relates to a rod section of a ground drilling rod, wherein the rod section is configured at the end to form a plug connection and has (a) a male connector or (b) a female connector at at least one end, wherein the male connector has at least one circular portion and at least one more straight-line-like portion in cross section on the outside, or the female connector has at least one circular portion and at least one more straight-line-like portion in cross section on the inside.

Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of interest comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and one or more trace element ions to the fermentation medium.

Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of inter-est comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and magnesium ions to the fermentation medium.

Abstract: The invention relates to a rod section of a ground drilling rod, wherein the rod section is configured at the end side to form at least one plug connection and has at one end (a) a male connector with an outer contour or (b) a female connector with an inner contour, wherein, in cross section, the outer contour or the inner contour has a shape deviating from a circle.

Abstract: A rod section of a ground drilling rod configured at its end to form at least one plug connection and having at one end (a) a connecting plug with an outer contour; or (b) a connecting socket with an inner contour, the outer contour or the inner contour being essentially sinusoidal in cross section.

Abstract: A device for the positioning of an electronic unit on a front-end section of a drill string of a ground drilling device includes a seat and a bearing surface. The electronic unit is designed to determine at least one of location, height, position and direction. The seat is configured for mounting or placement of the electronic unit onto the device. The bearing surface is adapted to be mounted to, or placed on, the outer contour of the front-end section to provide a predetermined positioning or reference between the seat and the front-end section of the drill string.

Abstract: A long-shaft tool includes a shaft extending along a longitudinal axis and having a handle at a first end of the shaft and a second end opposite the first end with a holder for a manipulator wherein the holder has a protruding latch pin provided for engagement with a recess of a manipulator, which can be moved from the protruding position into a retracted position. The holder has a coupling half for coupling with a coupling half of a manipulator which is rotatable relative to a base body of the shaft.

Abstract: A device for dampening the action of a force on an electronic component, particularly one in the form of a probe, wherein the device comprises a receptacle space for the component, which is surrounded by an outer wall along a longitudinal axis, and transversely to the longitudinal axis there is situated an end closure of the receptacle space, wherein the outer wall and the closure comprise the same material and the material has a structural conformation which produces a dampening effect of the device in the longitudinal direction.

Abstract: A rod section of a ground drilling rod, wherein the rod section is configured at its end as a plug connection and comprises at one end (a) a connection plug or (b) a connection socket, wherein the connection plug on the outside or the connection socket on the inside comprises nonround segments along a cross section, wherein the cross section is constant in configuration.

Abstract: A device for the positioning of an electronic unit on a front-end section of a drill string of a ground drilling device, wherein the electronic unit is designed to determine at least one of the following pieces of information: location, height, position and direction, and wherein the device has a seat for the electronic unit and a bearing surface adapted to the outer contour of the front-end section.