Abstract: A supply arrangement includes a supply reservoir element and a microfluidic device, wherein the supply reservoir element has a first reservoir unit having a prefilled liquid reagent therein, and the microfluidic device has a reception device adapted for receiving the liquid reagent from the first reservoir unit. The supply reservoir element is coupled to the microfluidic device. One of the supply reservoir element and the microfluidic device includes a puncturing element in order to cause fluid flow of the liquid reagent from the first reservoir unit to the reception device when the puncturing element is operated. The supply reservoir element has a basal plane, and the first reservoir unit has a first blister element arranged on the basal plane.

Abstract: Methods and devices for delivering fluids into microfluidic device body structures (112, 202, 316, 508) are described. The methods and devices include the use of fluid manifolds (200, 300, 400, 500) which are integrated or interchangeable with device body structures. Methods of fabricating manifolds are also provided.

Abstract: A microfluidic arrangement (1) for the optical detection of fluids is provided, comprising a microfluidic device (2) having at least one first channel (3) with an opening (4) which is in fluid communication with an optical detection unit (6) of an optical device (5); the microfluidic device (2) being operatively detachably coupled with the optical device (5) whereby an extension of the part (7,7?) of relevance of the optical detection path (17) is provided. A method for detecting fluids using the arrangement of the present invention is provided.

Abstract: A system and method for providing an improved microchip measurement apparatus comprising an external reservoir connected via an input line and output line to a well disposed on the microchip. The reservoir and well may be sealed and a pump connected to the input line so as to enable a continuous flow of liquid from the reservoir to the well. In addition, the volume of liquid in the well may be kept constant, thereby improving the overall measurement accuracy.

Abstract: The present invention provides an improved microchip measurement apparatus 15 comprising an external reservoir 24 connected via an input line 28 and output line 29 to a well 21 disposed on the microchip 20. The reservoir and well are preferable sealed and a pump 26 is connected to the input line so as to enable a continuous flow of liquid from the reservoir to the well. Advantageously, the present invention allows for the volume of liquid in the well to be kept constant, thereby improving the overall measurement accuracy.

Abstract: A transgenic mouse with alterations in a PA28&bgr; gene is prepared by introduction of an altered PA28&bgr; gene into a host mouse. The resulting transgenic animals do not produce functional PA28 molecules. Cells and cell lines derived from these animals also contain the altered PA28&bgr; gene.