Abstract: The present disclosure provides methods of treating psoriasis. The present disclosure provides methods of using a microneedle device in aid of treating a subject. The present disclosure also provides methods of treating a subject with psoriasis with a microneedle device. The present disclosure provides microneedle devices and kits for extracting biomarkers from a tissue. The present disclosure provides methods selecting a treatment for an autoimmune disease. The present disclosure also provides methods of nucleic acid analysis to prospectively predict a patient's response to a therapeutic.

Abstract: The present disclosure provides methods of treating psoriasis. The present disclosure provides methods of using a microneedle device in aid of treating a subject. The present disclosure also provides methods of treating a subject with psoriasis with a microneedle device. The present disclosure provides microneedle devices and kits for extracting biomarkers from a tissue. The present disclosure provides methods selecting a treatment for an autoimmune disease. The present disclosure also provides methods of nucleic acid analysis to prospectively predict a patient's response to a therapeutic.

Abstract: The present disclosure provides microneedle devices and kits for extracting biomarkers from a tissue. The present disclosure provides microneedle-device-mediated extraction methods for extracting biomarkers from a lesional skin of a subject. The present disclosure also provides computer-implemented methods for predicting a patient's response to an autoimmune therapeutic drug. The present disclosure provides methods selecting a treatment for an autoimmune disease. The present disclosure also provides methods of nucleic acid analysis to prospectively predict a patient's response to a therapeutic.

Abstract: The invention is directed to reliable and efficient detection of mRNAs as well as other RNAs in living cells and its use to identify and, if desired, separate cells based on their desired characteristics Such methods greatly simplify and reduce the time necessary to carry out previously-known procedures, and offers new approaches as well, such as selecting cells that generate a particular protein or antisense oligonucleotide, generating cell lines that express multiple proteins, generating cell lines with knock-out of one or more protein, and others.

Abstract: The invention provides methods and devices for the delivery of therapeutic or biologically active agents to tissue, for example by facilitating the transport of said agents through the skin of a human or animal. Therapeutic agents include various botulinum neurotoxin (BoNT) and other biologically active agents, which can be delivered across the skin and into the dermis using multiple strategies including microneedle drug delivery, transport moieties, or penetration enhancers such as extracellular matrix-digestive enzymes.