Abstract: Primers and probes derived from the 5′ untranslated region of the HCV genome which facilitate detection and/or quantification of all presently known genotypes of HCV. Disclosed sequences may be used in a variety of primer and probe constructs for amplification and/or detection of HCV nucleic acids.

Abstract: Primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). Disclosed sequences may be used in a variety of amplification and non-amplification formats for detection of HIV nucleic acids.

Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Abstract: The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

Abstract: Amplification primers and methods for specific amplification and detection of a P1 target are disclosed. The primer-target binding sequences are useful for amplification and detection of Mycoplasma pneumoniae target in a variety of amplification and detection reactions.

Abstract: Amplification primers and methods for specific amplification and detection of a pertussis toxin promoter target are disclosed. The primer-target binding sequences are useful for amplification and detection of Bordetella pertussis target in a variety of amplification and detection reactions.

Abstract: For use in nucleic acid amplification reactions, the detector oligonucleotides of the invention comprise a target binding sequence which is at least partially the same as the target binding sequence of an amplification primer present in the target amplification reaction, so that the detector oligonucleotide and the amplification primer compete for hybridization to the same sequence in the target. Hybridization of the amplification primer to the target upstream from the detector oligonucleotide generates a nickable restriction endonuclease recognition site. When this site is nicked and strand displacement occurs from the nick, both the 3′ end of the amplification primer and the detector oligonucleotide are displaced. The displaced detector oligonucleotide may then be detected as an indication of the presence of the target sequence, for example by unfolding of a fluorescently labeled secondary structure present in the detector oligonucleotide to reduce fluorescence quenching.

Abstract: Amplification primers and methods for specific amplification and detection of a mip gene sequence are disclosed. The primer-target binding sequences are useful for amplification and detection of Legionella pneumophila target in a variety of amplification and detection reactions.

Abstract: Amplification primers and methods for specific amplification and detection of a Shigella spp. and enteroinvasive stains of Escherichia coli (EIEC) target are disclosed. The primer-target binding sequences are useful for amplification and detection of Shigella and EIEC target in a variety of amplification and detection reactions.

Abstract: Amplification primers and methods for specific amplification and detection of a Salmonella spp. target are disclosed. The primer-target binding sequences are useful for amplification and detection of Salmonella target in a variety of amplification and detection reactions.

Abstract: Amplification primers and methods for specific amplification and detection of a Shiga-like toxin I (SLT-I) target are disclosed. The primer-target binding sequences are useful for amplification and detection of SLT-I target in a variety of amplification and detection reactions.

Abstract: Amplification primers and methods for specific amplification and detection of a Shigella spp. and enteroinvasive strains of Escherichia coli (EIEC) target are disclosed. The primer-target binding sequences are useful for amplification and detection of Shigella and EIEC target in a variety of amplification and detection reactions.