Patents by Inventor Toby Ost

Toby Ost has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR THE MULTIPLEXED ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID SEQUENCES
    Publication number: 20230183792
    Abstract: A method for the isothermal amplification of nucleic acid molecules, optionally on a solid support. The method uses single stranded nucleic acids having hairpin regions at both the 3? and 5? ends, or the extension products thereof.
    Type: Application
    Filed: August 7, 2020
    Publication date: June 15, 2023
    Applicant: DNAE DIAGNOSTICS LIMITED
    Inventor: Toby OST
  • Oxidizing agent for modified nucleotides
    Patent number: 10563248
    Abstract: This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. A first portion of the population is oxidised with a metal (VI) oxo complex and then the first portion and a second portion of said population are both treated with bisulfite. The residues in the first and second portions that correspond to a cytosine residue in the sample nucleotide sequence are identified following treatment and the identities of these residues are used to determine the modification of the cytosine residue in the sample nucleotide sequence. Methods, reagents and kits are provided.
    Type: Grant
    Filed: November 28, 2013
    Date of Patent: February 18, 2020
    Assignee: Cambridge Epigenetix Limited
    Inventor: Toby Ost
  • Oxidising Agent for Modified Nucleotides
    Publication number: 20190048406
    Abstract: This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. A first portion of the population is oxidised with a metal (VI) oxo complex and then the first portion and a second portion of said population are both treated with bisulfite. The residues in the first and second portions that correspond to a cytosine residue in the sample nucleotide sequence are identified following treatment and the identities of these residues are used to determine the modification of the cytosine residue in the sample nucleotide sequence. Methods, reagents and kits are provided.
    Type: Application
    Filed: October 26, 2018
    Publication date: February 14, 2019
    Inventor: Toby OST
  • OXIDIZING AGENT FOR MODIFIED NUCLEOTIDES
    Publication number: 20150299781
    Abstract: This invention relates to the use of metal (VI) oxo complexes to catalyse the selective oxidation of 5hmC residues in polynucleotides to 5fC residues. This may be useful in the identification of modified cytosine residues in a population of polynucleotides comprising a sample nucleotide sequence. A first portion of the population is oxidised with a metal (VI) oxo complex and then the first portion and a second portion of said population are both treated with bisulfite. The residues in the first and second portions that correspond to a cytosine residue in the sample nucleotide sequence are identified following treatment and the identities of these residues are used to determine the modification of the cytosine residue in the sample nucleotide sequence. Methods, reagents and kits are provided.
    Type: Application
    Filed: November 28, 2013
    Publication date: October 22, 2015
    Applicant: Cambridge Epigenetix Limited
    Inventor: Toby Ost